ID   RPC2B_CHLRE             Reviewed;         489 AA.
AC   Q8HUH0; B7U1K9;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta' C-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase beta' C-terminal section;
DE            Short=RNA polymerase beta' C-terminal section;
GN   Name=rpoC1B;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION, AND COMPLETE
RP   PLASTID GENOME.
RX   PubMed=12417694; DOI=10.1105/tpc.006155;
RA   Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA   Stern D.B.;
RT   "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT   sea of repeats.";
RL   Plant Cell 14:2659-2679(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA   Smith D.R., Lee R.W.;
RT   "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT   addressing the mutational-hazard hypothesis.";
RL   BMC Evol. Biol. 9:120-120(2009).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000250|UniProtKB:P0A8T7};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000250|UniProtKB:P0A8T7};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000250|UniProtKB:P0A8T7};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In C.reinhardtii the gene for this protein is split in
CC       two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AF541870; AAN17821.1; -; Genomic_DNA.
DR   EMBL; FJ423446; ACJ50156.1; -; Genomic_DNA.
DR   EMBL; BK000554; DAA00969.1; -; Genomic_DNA.
DR   RefSeq; NP_958425.1; NC_005353.1.
DR   AlphaFoldDB; Q8HUH0; -.
DR   SMR; Q8HUH0; -.
DR   STRING; 3055.DAA00969; -.
DR   PaxDb; Q8HUH0; -.
DR   PRIDE; Q8HUH0; -.
DR   GeneID; 2717049; -.
DR   KEGG; cre:ChreCp069; -.
DR   eggNOG; ENOG502QPYA; Eukaryota.
DR   HOGENOM; CLU_558214_0_0_1; -.
DR   InParanoid; Q8HUH0; -.
DR   OrthoDB; 774084at2759; -.
DR   Proteomes; UP000006906; Chloroplast.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.274.100; -; 1.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR000722; RNA_pol_asu.
DR   InterPro; IPR006592; RNA_pol_N.
DR   InterPro; IPR007080; RNA_pol_Rpb1_1.
DR   InterPro; IPR007066; RNA_pol_Rpb1_3.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR   Pfam; PF00623; RNA_pol_Rpb1_2; 2.
DR   Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR   SMART; SM00663; RPOLA_N; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; DNA-directed RNA polymerase; Magnesium; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Reference proteome; Transcription;
KW   Transferase.
FT   CHAIN           1..489
FT                   /note="DNA-directed RNA polymerase subunit beta' C-terminal
FT                   section"
FT                   /id="PRO_0000067867"
FT   BINDING         208
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:P0A8T7"
FT   BINDING         210
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:P0A8T7"
FT   BINDING         212
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:P0A8T7"
SQ   SEQUENCE   489 AA;  55630 MW;  1912E0FB97448C47 CRC64;
     MSGQFTISDL NRLYQRIIYR NERLKKFLKD PALSSSFEMK YAQRLLQEAV DNLIQNGKSG
     VVPEKDARGR LLKSLSDILK GKQGRFRQYL LGKRVDYSGR SVIVVGPRLR LHECGIPKEM
     ALVLYSPFLI KRILNEKLAD TYLSAKKLIR TNPLLVSQLL REIMKSCPVL LNRAPTLHRL
     GFQAFQPKLV DGKAILLHPL VCPAFNADFD GDQMAVHVPI TFEARAEAWK LMLARNNLLS
     PATGEPLILP SQDMVLGCYY LTTNCAEKWS KLKKGSGMYF HNIHEVLKAY NQHLIHLHAV
     IWVNIQGHLE TANNIEQPLE IRIPLNQKYF YSQKGSYTAL AGRPWQLLLR EDAYSSNGLI
     AEANAGNLPQ INVAEGEVNK NILDLRYLNL NYMEIYSKTH NLLNVNHSTL VAQRGQIKDN
     IHLPQLTTQI IRTTPGKILF NIIVKNAIEK RPKLLSKSNF KGGFIKNKLV NAIDQEIDNY
     FIRKNTALS