ID   RPC2_BPP22              Reviewed;         216 AA.
AC   P69202; P03035; Q7PCF2;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   23-FEB-2022, entry version 86.
DE   RecName: Full=Repressor protein C2;
GN   Name=C2;
OS   Salmonella phage P22 (Bacteriophage P22).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Lederbergvirus.
OX   NCBI_TaxID=10754;
OH   NCBI_TaxID=90371; Salmonella typhimurium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE.
RX   PubMed=7260059; DOI=10.1021/bi00515a044;
RA   Sauer R.T., Pan J., Hopper P., Hehir K., Brown J., Poteete A.R.;
RT   "Primary structure of the phage P22 repressor and its gene c2.";
RL   Biochemistry 20:3591-3598(1981).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11053393; DOI=10.1128/jb.182.22.6472-6481.2000;
RA   Vander Byl C.S., Kropinski A.M.B.;
RT   "Sequence of the genome of Salmonella bacteriophage P22.";
RL   J. Bacteriol. 182:6472-6481(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12562822; DOI=10.1128/jb.185.4.1475-1477.2003;
RA   Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W.,
RA   Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A.,
RA   Casjens S.R.;
RT   "Corrected sequence of the bacteriophage P22 genome.";
RL   J. Bacteriol. 185:1475-1477(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
RX   PubMed=6445008; DOI=10.1016/0022-2836(80)90158-8;
RA   Poteete A.R., Ptashne M., Ballivet M., Eisen H.;
RT   "Operator sequences of bacteriophages P22 and 21.";
RL   J. Mol. Biol. 137:81-91(1980).
RN   [5]
RP   STRUCTURE BY NMR OF 1-76.
RX   PubMed=8289306; DOI=10.1006/jmbi.1994.1053;
RA   Sevilla-Sierra P., Otting G., Wuethrich K.;
RT   "Determination of the nuclear magnetic resonance structure of the DNA-
RT   binding domain of the P22 c2 repressor (1 to 76) in solution and comparison
RT   with the DNA-binding domain of the 434 repressor.";
RL   J. Mol. Biol. 235:1003-1020(1994).
CC   -!- FUNCTION: This protein allows the phage to reside inactively in the
CC       chromosome of its host bacterium. This lysogenic state is maintained by
CC       binding of regulatory protein C2 to the OR and OL operators, preventing
CC       transcription of proteins necessary for lytic development.
CC   -!- PTM: In the mature form of the repressor protein the initiator
CC       methionine is deformylated.
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DR   EMBL; V01153; CAA24470.1; -; Genomic_DNA.
DR   EMBL; AF217253; AAF75024.1; -; Genomic_DNA.
DR   EMBL; BK000583; DAA01021.1; -; Genomic_DNA.
DR   PIR; A03571; RPBP22.
DR   RefSeq; NP_059606.1; NC_002371.2.
DR   PDB; 1ADR; NMR; -; A=1-76.
DR   PDB; 2R1J; X-ray; 1.53 A; L/R=1-68.
DR   PDB; 3JXB; X-ray; 1.67 A; C/D=2-68.
DR   PDB; 3JXC; X-ray; 1.90 A; L/R=2-68.
DR   PDB; 3JXD; X-ray; 2.10 A; L/R=2-68.
DR   PDBsum; 1ADR; -.
DR   PDBsum; 2R1J; -.
DR   PDBsum; 3JXB; -.
DR   PDBsum; 3JXC; -.
DR   PDBsum; 3JXD; -.
DR   SMR; P69202; -.
DR   MEROPS; S24.002; -.
DR   GeneID; 1262793; -.
DR   KEGG; vg:1262793; -.
DR   EvolutionaryTrace; P69202; -.
DR   Proteomes; UP000001795; Genome.
DR   Proteomes; UP000007960; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   CDD; cd00093; HTH_XRE; 1.
DR   CDD; cd06529; S24_LexA-like; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR039418; LexA-like.
DR   InterPro; IPR036286; LexA/Signal_pep-like_sf.
DR   InterPro; IPR015927; Peptidase_S24_S26A/B/C.
DR   Pfam; PF01381; HTH_3; 1.
DR   Pfam; PF00717; Peptidase_S24; 1.
DR   SMART; SM00530; HTH_XRE; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   SUPFAM; SSF51306; SSF51306; 1.
DR   PROSITE; PS50943; HTH_CROC1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; DNA-binding; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..216
FT                   /note="Repressor protein C2"
FT                   /id="PRO_0000149717"
FT   DOMAIN          10..64
FT                   /note="HTH cro/C1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   DNA_BIND        21..40
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   SITE            1
FT                   /note="Not N-formylated"
FT   HELIX           6..17
FT                   /evidence="ECO:0007829|PDB:2R1J"
FT   HELIX           21..28
FT                   /evidence="ECO:0007829|PDB:2R1J"
FT   HELIX           32..39
FT                   /evidence="ECO:0007829|PDB:2R1J"
FT   HELIX           47..56
FT                   /evidence="ECO:0007829|PDB:2R1J"
FT   HELIX           61..66
FT                   /evidence="ECO:0007829|PDB:2R1J"
FT   TURN            73..75
FT                   /evidence="ECO:0007829|PDB:1ADR"
SQ   SEQUENCE   216 AA;  24069 MW;  85A32EE9DB785E09 CRC64;
     MNTQLMGERI RARRKKLKIR QAALGKMVGV SNVAISQWER SETEPNGENL LALSKALQCS
     PDYLLKGDLS QTNVAYHSRH EPRGSYPLIS WVSAGQWMEA VEPYHKRAIE NWHDTTVDCS
     EDSFWLDVQG DSMTAPAGLS IPEGMIILVD PEVEPRNGKL VVAKLEGENE ATFKKLVMDA
     GRKFLKPLNP QYPMIEINGN CKIIGVVVDA KLANLP