RPC5_YEAST
ID RPC5_YEAST Reviewed; 282 AA.
AC P36121; D6VX90;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=DNA-directed RNA polymerase III subunit RPC5;
DE Short=RNA polymerase III subunit C5;
DE AltName: Full=DNA-directed RNA polymerase III 37 kDa polypeptide;
DE AltName: Full=RNA polymerase III subunit C37;
GN Name=RPC37; OrderedLocusNames=YKR025W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP REVIEW ON THE RNA POL III COMPLEX.
RX PubMed=10384303; DOI=10.1101/sqb.1998.63.381;
RA Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O.,
RA Werner M., Carles C., Sentenac A.;
RT "The yeast RNA polymerase III transcription machinery: a paradigm for
RT eukaryotic gene activation.";
RL Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998).
RN [5]
RP IDENTIFICATION IN THE RNA POL III COMPLEX, AND INTERACTION WITH RPC4.
RX PubMed=10393904; DOI=10.1073/pnas.96.14.7815;
RA Flores A., Briand J.-F., Gadal O., Andrau J.-C., Rubbi L., Van Mullem V.,
RA Boschiero C., Goussot M., Marck C., Carles C., Thuriaux P., Sentenac A.,
RA Werner M.;
RT "A protein-protein interaction map of yeast RNA polymerase III.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:7815-7820(1999).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP FUNCTION OF THE RPC53-RPC37 SUBCOMPLEX.
RX PubMed=16362040; DOI=10.1038/sj.emboj.7600915;
RA Landrieux E., Alic N., Ducrot C., Acker J., Riva M., Carles C.;
RT "A subcomplex of RNA polymerase III subunits involved in transcription
RT termination and reinitiation.";
RL EMBO J. 25:118-128(2006).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-61, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-61, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Specific peripheric component of RNA polymerase III which synthesizes
CC small RNAs, such as 5S rRNA and tRNAs. The RPC53/RPC4-RPC37/RPC5
CC subcomplex is required for terminator recognition and reinitiation.
CC {ECO:0000269|PubMed:16362040}.
CC -!- SUBUNIT: Component of the RNA polymerase III (Pol III) complex
CC consisting of 17 subunits. Interacts with RPC53/RPC4. RPC53/RPC4,
CC RPC37/RPC5 and RPC11/RPC10 probably form a Pol III subcomplex.
CC {ECO:0000269|PubMed:10393904}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1590 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; Z28250; CAA82097.1; -; Genomic_DNA.
DR EMBL; AY692886; AAT92905.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09180.1; -; Genomic_DNA.
DR PIR; S38094; S38094.
DR RefSeq; NP_012950.3; NM_001179815.3.
DR PDB; 5FJ8; EM; 3.90 A; M=1-282.
DR PDB; 5FJ9; EM; 4.60 A; M=1-282.
DR PDB; 5FJA; EM; 4.65 A; M=1-282.
DR PDB; 6CNB; EM; 4.10 A; M=1-282.
DR PDB; 6CNC; EM; 4.10 A; M=1-282.
DR PDB; 6CND; EM; 4.80 A; M=1-282.
DR PDB; 6CNF; EM; 4.50 A; M=1-282.
DR PDB; 6EU0; EM; 4.00 A; M=1-282.
DR PDB; 6EU1; EM; 3.40 A; M=1-282.
DR PDB; 6EU2; EM; 3.40 A; M=1-282.
DR PDB; 6EU3; EM; 3.30 A; M=1-282.
DR PDB; 6F40; EM; 3.70 A; M=1-282.
DR PDB; 6F41; EM; 4.30 A; M=1-282.
DR PDB; 6F42; EM; 5.50 A; M=1-282.
DR PDB; 6F44; EM; 4.20 A; M=1-282.
DR PDB; 6TUT; EM; 3.25 A; M=1-282.
DR PDBsum; 5FJ8; -.
DR PDBsum; 5FJ9; -.
DR PDBsum; 5FJA; -.
DR PDBsum; 6CNB; -.
DR PDBsum; 6CNC; -.
DR PDBsum; 6CND; -.
DR PDBsum; 6CNF; -.
DR PDBsum; 6EU0; -.
DR PDBsum; 6EU1; -.
DR PDBsum; 6EU2; -.
DR PDBsum; 6EU3; -.
DR PDBsum; 6F40; -.
DR PDBsum; 6F41; -.
DR PDBsum; 6F42; -.
DR PDBsum; 6F44; -.
DR PDBsum; 6TUT; -.
DR AlphaFoldDB; P36121; -.
DR SMR; P36121; -.
DR BioGRID; 34157; 67.
DR ComplexPortal; CPX-2660; DNA-directed RNA polymerase III complex.
DR DIP; DIP-761N; -.
DR IntAct; P36121; 11.
DR MINT; P36121; -.
DR STRING; 4932.YKR025W; -.
DR iPTMnet; P36121; -.
DR MaxQB; P36121; -.
DR PaxDb; P36121; -.
DR PRIDE; P36121; -.
DR EnsemblFungi; YKR025W_mRNA; YKR025W; YKR025W.
DR GeneID; 853895; -.
DR KEGG; sce:YKR025W; -.
DR SGD; S000001733; RPC37.
DR VEuPathDB; FungiDB:YKR025W; -.
DR eggNOG; KOG2354; Eukaryota.
DR GeneTree; ENSGT00390000016123; -.
DR HOGENOM; CLU_072845_1_0_1; -.
DR InParanoid; P36121; -.
DR OMA; PKTGWME; -.
DR BioCyc; YEAST:G3O-32001-MON; -.
DR PRO; PR:P36121; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36121; protein.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0005666; C:RNA polymerase III complex; IDA:SGD.
DR GO; GO:0006386; P:termination of RNA polymerase III transcription; IDA:ComplexPortal.
DR GO; GO:0006383; P:transcription by RNA polymerase III; IDA:ComplexPortal.
DR GO; GO:0006384; P:transcription initiation from RNA polymerase III promoter; IDA:ComplexPortal.
DR GO; GO:0042797; P:tRNA transcription by RNA polymerase III; IDA:SGD.
DR InterPro; IPR006886; RNA_pol_III_Rpc5.
DR PANTHER; PTHR12069; PTHR12069; 1.
DR Pfam; PF04801; RPC5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription.
FT CHAIN 1..282
FT /note="DNA-directed RNA polymerase III subunit RPC5"
FT /id="PRO_0000203203"
FT REGION 1..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..33
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 53..70
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 61
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT STRAND 70..74
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 86..101
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:6TUT"
FT TURN 114..117
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 118..124
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 127..131
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 134..139
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 145..154
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 158..164
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 167..172
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 175..182
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 184..191
FT /evidence="ECO:0007829|PDB:6TUT"
FT TURN 192..197
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 230..238
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 246..249
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 253..257
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 259..261
FT /evidence="ECO:0007829|PDB:6TUT"
FT TURN 262..264
FT /evidence="ECO:0007829|PDB:6EU1"
SQ SEQUENCE 282 AA; 32142 MW; 9E948BDDECE9D15E CRC64;
MSIDNKLFVT EEDEEDRTQD RADVEDESND IDMIADENGT NSAIANEQEE KSEEVKAEDD
TGEEEEDDPV IEEFPLKISG EEESLHVFQY ANRPRLVGRK PAEHPFISAA RYKPKSHLWE
IDIPLDEQAF YNKDKAESEW NGVNVQTLKG VGVENNGQYA AFVKDMQVYL VPIERVAQLK
PFFKYIDDAN VTRKQEDARR NPNPSSQRAQ VVTMSVKSVN DPSQNRLTGS LLAHKVADEE
ANIELTWAEG TFEQFKDTIV KEAEDKTLVA LEKQEDYIDN LV
