RPC6_YEAST
ID RPC6_YEAST Reviewed; 317 AA.
AC P32910; D6W1H9;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=DNA-directed RNA polymerase III subunit RPC6;
DE Short=RNA polymerase III subunit C6;
DE AltName: Full=C34;
DE AltName: Full=DNA-directed RNA polymerase III 36 kDa polypeptide;
GN Name=RPC34; OrderedLocusNames=YNR003C; ORFNames=N2031;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 26-38 AND
RP 214-219.
RC STRAIN=S288c / GRF88;
RX PubMed=1400451; DOI=10.1016/s0021-9258(19)36622-0;
RA Stettler S., Mariotte S., Riva M., Sentenac A., Thuriaux P.;
RT "An essential and specific subunit of RNA polymerase III (C) is encoded by
RT gene RPC34 in Saccharomyces cerevisiae.";
RL J. Biol. Chem. 267:21390-21395(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=S288c / GRF88;
RX PubMed=7941739; DOI=10.1002/yea.320100412;
RA Lalo D., Stettler S., Mariotte S., Gendreau E., Thuriaux P.;
RT "Organization of the centromeric region of chromosome XIV in Saccharomyces
RT cerevisiae.";
RL Yeast 10:523-533(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=7900425; DOI=10.1002/yea.320101013;
RA Verhasselt P., Aert R., Voet M., Volckaert G.;
RT "Twelve open reading frames revealed in the 23.6 kb segment flanking the
RT centromere on the Saccharomyces cerevisiae chromosome XIV right arm.";
RL Yeast 10:1355-1361(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP INTERACTION WITH BRF1.
RX PubMed=8407894; DOI=10.1016/s0021-9258(19)36839-5;
RA Werner M., Chaussivert N., Willis I.M., Sentenac A.;
RT "Interaction between a complex of RNA polymerase III subunits and the 70-
RT kDa component of transcription factor IIIB.";
RL J. Biol. Chem. 268:20721-20724(1993).
RN [7]
RP FUNCTION, INTERACTION WITH BRF1, AND MUTAGENESIS OF GLU-89;
RP 102-ARG-GLU-103; LYS-135 AND 171-ASP-GLU-173.
RX PubMed=9312031; DOI=10.1093/emboj/16.18.5730;
RA Brun I., Sentenac A., Werner M.;
RT "Dual role of the C34 subunit of RNA polymerase III in transcription
RT initiation.";
RL EMBO J. 16:5730-5741(1997).
RN [8]
RP REVIEW ON THE RNA POL III COMPLEX.
RX PubMed=10384303; DOI=10.1101/sqb.1998.63.381;
RA Chedin S., Ferri M.L., Peyroche G., Andrau J.-C., Jourdain S., Lefebvre O.,
RA Werner M., Carles C., Sentenac A.;
RT "The yeast RNA polymerase III transcription machinery: a paradigm for
RT eukaryotic gene activation.";
RL Cold Spring Harb. Symp. Quant. Biol. 63:381-389(1998).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Specific peripheric component of RNA polymerase III which synthesizes
CC small RNAs, such as 5S rRNA and tRNAs. Involved in recruitment of Pol
CC III to the preinitiation complex. Involved in the configuration of an
CC initiation-competent form of RNA polymerase.
CC {ECO:0000269|PubMed:9312031}.
CC -!- SUBUNIT: Component of the RNA polymerase III (Pol III) complex
CC consisting of 17 subunits. Interacts with BRF1/TDS4.
CC {ECO:0000269|PubMed:8407894, ECO:0000269|PubMed:9312031}.
CC -!- INTERACTION:
CC P32910; P41910: MAF1; NbExp=3; IntAct=EBI-15835, EBI-10375;
CC P32910; P20436: RPB8; NbExp=3; IntAct=EBI-15835, EBI-15794;
CC P32910; P47076: RPC17; NbExp=2; IntAct=EBI-15835, EBI-25782;
CC P32910; P32349: RPC82; NbExp=3; IntAct=EBI-15835, EBI-15821;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the eukaryotic RPC34/RPC39 RNA polymerase
CC subunit family. {ECO:0000305}.
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DR EMBL; X63746; CAA45278.1; -; Genomic_DNA.
DR EMBL; X77395; CAA54572.1; -; Genomic_DNA.
DR EMBL; Z71618; CAA96279.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10545.1; -; Genomic_DNA.
DR PIR; A45107; A45107.
DR RefSeq; NP_014400.1; NM_001183180.1.
DR PDB; 5FJ8; EM; 3.90 A; P=1-317.
DR PDB; 5FJ9; EM; 4.60 A; P=1-317.
DR PDB; 5FJA; EM; 4.65 A; P=1-317.
DR PDB; 6CNB; EM; 4.10 A; P=1-317.
DR PDB; 6CNC; EM; 4.10 A; P=1-317.
DR PDB; 6CND; EM; 4.80 A; P=1-317.
DR PDB; 6CNF; EM; 4.50 A; P=1-317.
DR PDB; 6EU0; EM; 4.00 A; P=1-317.
DR PDB; 6EU1; EM; 3.40 A; P=1-317.
DR PDB; 6EU2; EM; 3.40 A; P=1-317.
DR PDB; 6EU3; EM; 3.30 A; P=1-317.
DR PDB; 6F40; EM; 3.70 A; P=1-317.
DR PDB; 6F41; EM; 4.30 A; P=1-317.
DR PDB; 6F42; EM; 5.50 A; P=1-317.
DR PDB; 6F44; EM; 4.20 A; P=1-317.
DR PDB; 6TUT; EM; 3.25 A; P=1-317.
DR PDBsum; 5FJ8; -.
DR PDBsum; 5FJ9; -.
DR PDBsum; 5FJA; -.
DR PDBsum; 6CNB; -.
DR PDBsum; 6CNC; -.
DR PDBsum; 6CND; -.
DR PDBsum; 6CNF; -.
DR PDBsum; 6EU0; -.
DR PDBsum; 6EU1; -.
DR PDBsum; 6EU2; -.
DR PDBsum; 6EU3; -.
DR PDBsum; 6F40; -.
DR PDBsum; 6F41; -.
DR PDBsum; 6F42; -.
DR PDBsum; 6F44; -.
DR PDBsum; 6TUT; -.
DR AlphaFoldDB; P32910; -.
DR SMR; P32910; -.
DR BioGRID; 35829; 477.
DR ComplexPortal; CPX-2660; DNA-directed RNA polymerase III complex.
DR DIP; DIP-202N; -.
DR IntAct; P32910; 23.
DR MINT; P32910; -.
DR STRING; 4932.YNR003C; -.
DR MaxQB; P32910; -.
DR PaxDb; P32910; -.
DR PRIDE; P32910; -.
DR EnsemblFungi; YNR003C_mRNA; YNR003C; YNR003C.
DR GeneID; 855737; -.
DR KEGG; sce:YNR003C; -.
DR SGD; S000005286; RPC34.
DR VEuPathDB; FungiDB:YNR003C; -.
DR eggNOG; KOG3233; Eukaryota.
DR GeneTree; ENSGT00390000009679; -.
DR HOGENOM; CLU_033661_0_1_1; -.
DR InParanoid; P32910; -.
DR OMA; AVYFDEW; -.
DR BioCyc; YEAST:G3O-33322-MON; -.
DR Reactome; R-SCE-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-SCE-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR PRO; PR:P32910; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P32910; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005739; C:mitochondrion; IPI:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005666; C:RNA polymerase III complex; IDA:SGD.
DR GO; GO:0006386; P:termination of RNA polymerase III transcription; IDA:ComplexPortal.
DR GO; GO:0006383; P:transcription by RNA polymerase III; IDA:ComplexPortal.
DR GO; GO:0006384; P:transcription initiation from RNA polymerase III promoter; IDA:ComplexPortal.
DR GO; GO:0042797; P:tRNA transcription by RNA polymerase III; IDA:SGD.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR007832; RNA_pol_Rpc34.
DR InterPro; IPR016049; RNA_pol_Rpc34-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12780; PTHR12780; 1.
DR Pfam; PF05158; RNA_pol_Rpc34; 1.
DR PIRSF; PIRSF028763; RNA_pol_Rpc34; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA-directed RNA polymerase;
KW Nucleus; Reference proteome; Transcription.
FT CHAIN 1..317
FT /note="DNA-directed RNA polymerase III subunit RPC6"
FT /id="PRO_0000073977"
FT MUTAGEN 89
FT /note="E->A: Cold-sensitive. Abolishes interaction with
FT BRF1/TDS4."
FT /evidence="ECO:0000269|PubMed:9312031"
FT MUTAGEN 102..103
FT /note="RE->VA: Cold-sensitive. No effect on interaction
FT with BRF1/TDS4."
FT /evidence="ECO:0000269|PubMed:9312031"
FT MUTAGEN 135..138
FT /note="KSVK->ASVA: Temperature-sensitive; cold-sensitive.
FT Abolishes interaction with BRF1/TDS4. Stabilizes Pol III
FT open complex formation."
FT MUTAGEN 135
FT /note="K->A: Cold-sensitive. Abolishes interaction with
FT BRF1/TDS4."
FT /evidence="ECO:0000269|PubMed:9312031"
FT MUTAGEN 171..173
FT /note="DIE->AIA: Cold-sensitive. Abolishes interaction with
FT BRF1/TDS4."
FT /evidence="ECO:0000269|PubMed:9312031"
FT MUTAGEN 171
FT /note="D->H: Cold-sensitive. Abolishes interaction with
FT BRF1/TDS4."
FT HELIX 92..100
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 120..130
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 140..145
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 147..151
FT /evidence="ECO:0007829|PDB:6TUT"
FT TURN 171..174
FT /evidence="ECO:0007829|PDB:6EU1"
FT HELIX 175..188
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 193..195
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 196..198
FT /evidence="ECO:0007829|PDB:6EU3"
FT HELIX 202..208
FT /evidence="ECO:0007829|PDB:6EU3"
FT HELIX 218..227
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 231..233
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 237..239
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 240..243
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 244..246
FT /evidence="ECO:0007829|PDB:6TUT"
FT TURN 247..251
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 252..256
FT /evidence="ECO:0007829|PDB:6TUT"
FT TURN 257..259
FT /evidence="ECO:0007829|PDB:6TUT"
FT STRAND 260..262
FT /evidence="ECO:0007829|PDB:6TUT"
FT HELIX 266..269
FT /evidence="ECO:0007829|PDB:6TUT"
FT TURN 270..275
FT /evidence="ECO:0007829|PDB:6EU1"
FT STRAND 300..302
FT /evidence="ECO:0007829|PDB:6EU1"
FT STRAND 303..309
FT /evidence="ECO:0007829|PDB:6EU3"
FT STRAND 311..313
FT /evidence="ECO:0007829|PDB:6EU3"
SQ SEQUENCE 317 AA; 36135 MW; BDE5CD6FD08BDA7F CRC64;
MSGMIENGLQ LSDNAKTLHS QMMSKGIGAL FTQQELQKQM GIGSLTDLMS IVQELLDKNL
IKLVKQNDEL KFQGVLESEA QKKATMSAEE ALVYSYIEAS GREGIWSKTI KARTNLHQHV
VLKCLKSLES QRYVKSVKSV KFPTRKIYML YSLQPSVDIT GGPWFTDGEL DIEFINSLLT
IVWRFISENT FPNGFKNFEN GPKKNVFYAP NVKNYSTTQE ILEFITAAQV ANVELTPSNI
RSLCEVLVYD DKLEKVTHDC YRVTLESILQ MNQGEGEPEA GNKALEDEEE FSIFNYFKMF
PASKHDKEVV YFDEWTI
