RPC8_MOUSE
ID RPC8_MOUSE Reviewed; 204 AA.
AC Q9D2C6; Q91XE5;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=DNA-directed RNA polymerase III subunit RPC8;
DE AltName: Full=DNA-directed RNA polymerase III subunit H;
DE AltName: Full=RNA polymerase III subunit C7;
GN Name=Polr3h;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Ovary, and Uterus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC Specific peripheric component of RNA polymerase III which synthesizes
CC small RNAs, such as 5S rRNA and tRNA. Plays a key role in sensing and
CC limiting infection by intracellular bacteria and DNA viruses. Acts as
CC nuclear and cytosolic DNA sensor involved in innate immune response.
CC Can sense non-self dsDNA that serves as template for transcription into
CC dsRNA. The non-self RNA polymerase III transcripts induce type I
CC interferon and NF- Kappa-B through the RIG-I pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the RNA polymerase III (Pol III) complex
CC consisting of 17 subunits. Interacts with RPC9 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit
CC family. {ECO:0000305}.
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DR EMBL; AK019868; BAB31893.2; -; mRNA.
DR EMBL; BC010793; AAH10793.1; -; mRNA.
DR CCDS; CCDS27676.1; -.
DR RefSeq; NP_084505.2; NM_030229.4.
DR AlphaFoldDB; Q9D2C6; -.
DR SMR; Q9D2C6; -.
DR BioGRID; 219714; 7.
DR STRING; 10090.ENSMUSP00000023113; -.
DR PhosphoSitePlus; Q9D2C6; -.
DR EPD; Q9D2C6; -.
DR MaxQB; Q9D2C6; -.
DR PaxDb; Q9D2C6; -.
DR PRIDE; Q9D2C6; -.
DR ProteomicsDB; 260837; -.
DR Antibodypedia; 26963; 166 antibodies from 25 providers.
DR DNASU; 78929; -.
DR Ensembl; ENSMUST00000023113; ENSMUSP00000023113; ENSMUSG00000022476.
DR GeneID; 78929; -.
DR KEGG; mmu:78929; -.
DR UCSC; uc007wxr.1; mouse.
DR CTD; 171568; -.
DR MGI; MGI:1926179; Polr3h.
DR VEuPathDB; HostDB:ENSMUSG00000022476; -.
DR eggNOG; KOG3297; Eukaryota.
DR GeneTree; ENSGT00390000004383; -.
DR HOGENOM; CLU_073901_1_0_1; -.
DR InParanoid; Q9D2C6; -.
DR OMA; LGPTLWW; -.
DR OrthoDB; 1430056at2759; -.
DR PhylomeDB; Q9D2C6; -.
DR TreeFam; TF103053; -.
DR Reactome; R-MMU-76061; RNA Polymerase III Transcription Initiation From Type 1 Promoter.
DR Reactome; R-MMU-76066; RNA Polymerase III Transcription Initiation From Type 2 Promoter.
DR Reactome; R-MMU-76071; RNA Polymerase III Transcription Initiation From Type 3 Promoter.
DR BioGRID-ORCS; 78929; 25 hits in 70 CRISPR screens.
DR ChiTaRS; Polr3h; mouse.
DR PRO; PR:Q9D2C6; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9D2C6; protein.
DR Bgee; ENSMUSG00000022476; Expressed in hindlimb stylopod muscle and 255 other tissues.
DR ExpressionAtlas; Q9D2C6; baseline and differential.
DR Genevisible; Q9D2C6; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0005666; C:RNA polymerase III complex; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; ISS:UniProtKB.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0006139; P:nucleobase-containing compound metabolic process; ISS:UniProtKB.
DR GO; GO:0006383; P:transcription by RNA polymerase III; ISS:UniProtKB.
DR GO; GO:0006384; P:transcription initiation from RNA polymerase III promoter; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.1490.120; -; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR013238; RNA_pol_III_Rbc25.
DR InterPro; IPR036898; RNA_pol_Rpb7-like_N_sf.
DR InterPro; IPR004519; RNAP_E/RPC8.
DR InterPro; IPR045113; Rpb7-like.
DR InterPro; IPR005576; Rpb7-like_N.
DR PANTHER; PTHR12709; PTHR12709; 1.
DR Pfam; PF08292; RNA_pol_Rbc25; 1.
DR Pfam; PF03876; SHS2_Rpb7-N; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF88798; SSF88798; 1.
DR TIGRFAMs; TIGR00448; rpoE; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; DNA-directed RNA polymerase; Immunity; Innate immunity;
KW Nucleus; Reference proteome; Transcription.
FT CHAIN 1..204
FT /note="DNA-directed RNA polymerase III subunit RPC8"
FT /id="PRO_0000073995"
SQ SEQUENCE 204 AA; 22948 MW; C63B2E92998A75D9 CRC64;
MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD ITKLEDAYVF
PGDGASHTKV HFRYVVFHPF LDEILIGKIK GCSPEGVHVS LGFFDDILIP PESLQQPAKF
DEAEQVWVWE YETEEGAHDL YMDTGEEIRF RVVDESFVDT SPTGPSSAEA ASSSEELPKK
EAPYTLVGSI SEPGLGLLSW WTSN
