RPF2_DROME
ID RPF2_DROME Reviewed; 320 AA.
AC Q9VEB3; Q95U84;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 04-AUG-2003, sequence version 3.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Ribosome production factor 2 homolog;
DE AltName: Full=Brix domain-containing protein 1 homolog;
DE AltName: Full=Novel nucleolar protein 3 {ECO:0000303|PubMed:24255106};
DE AltName: Full=Ribosome biogenesis protein RPF2 homolog;
GN Name=Non3 {ECO:0000312|FlyBase:FBgn0038585};
GN ORFNames=CG7993 {ECO:0000312|FlyBase:FBgn0038585};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24255106; DOI=10.1073/pnas.1320013110;
RA Moutinho-Pereira S., Stuurman N., Afonso O., Hornsveld M., Aguiar P.,
RA Goshima G., Vale R.D., Maiato H.;
RT "Genes involved in centrosome-independent mitotic spindle assembly in
RT Drosophila S2 cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:19808-19813(2013).
CC -!- FUNCTION: Required for normal assembly of the mitotic spindle. May be
CC involved in both centrosome-dependent and centrosome-independent
CC spindle assembly programs. {ECO:0000269|PubMed:24255106}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:24255106}.
CC Note=Found in the nucleolus during interphase.
CC {ECO:0000269|PubMed:24255106}.
CC -!- SIMILARITY: Belongs to the RPF2 family. {ECO:0000305}.
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DR EMBL; AE014297; AAF55514.2; -; Genomic_DNA.
DR EMBL; AY058248; AAL13477.1; -; mRNA.
DR RefSeq; NP_650694.2; NM_142437.3.
DR AlphaFoldDB; Q9VEB3; -.
DR SMR; Q9VEB3; -.
DR BioGRID; 67204; 4.
DR IntAct; Q9VEB3; 2.
DR STRING; 7227.FBpp0082984; -.
DR PaxDb; Q9VEB3; -.
DR EnsemblMetazoa; FBtr0083562; FBpp0082984; FBgn0038585.
DR GeneID; 42183; -.
DR KEGG; dme:Dmel_CG7993; -.
DR UCSC; CG7993-RA; d. melanogaster.
DR CTD; 42183; -.
DR FlyBase; FBgn0038585; Non3.
DR VEuPathDB; VectorBase:FBgn0038585; -.
DR eggNOG; KOG3031; Eukaryota.
DR GeneTree; ENSGT00390000007279; -.
DR HOGENOM; CLU_049783_1_1_1; -.
DR InParanoid; Q9VEB3; -.
DR OMA; GCKIPRI; -.
DR OrthoDB; 974865at2759; -.
DR PhylomeDB; Q9VEB3; -.
DR BioGRID-ORCS; 42183; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 42183; -.
DR PRO; PR:Q9VEB3; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038585; Expressed in adult abdomen and 57 other tissues.
DR ExpressionAtlas; Q9VEB3; baseline and differential.
DR Genevisible; Q9VEB3; DM.
DR GO; GO:0005730; C:nucleolus; IDA:FlyBase.
DR GO; GO:0019843; F:rRNA binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR InterPro; IPR007109; Brix.
DR InterPro; IPR039770; Rpf2.
DR PANTHER; PTHR12728; PTHR12728; 1.
DR Pfam; PF04427; Brix; 1.
DR SMART; SM00879; Brix; 1.
DR PROSITE; PS50833; BRIX; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..320
FT /note="Ribosome production factor 2 homolog"
FT /id="PRO_0000120226"
FT DOMAIN 30..234
FT /note="Brix"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00034"
FT REGION 268..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..302
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 188
FT /note="T -> I (in Ref. 3; AAL13477)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 36498 MW; EE988944DD5226F3 CRC64;
MSLLRIRKPK TRKGKKVLLA REPQLIESAR TMLFLDGRKC GGNVKLCMKD LQALKKPLVK
VLNRKNDITP FDDPSSLEFL TMKNDAALFT FGSTSKKRPD NIILGRIFEN EVLDMFELGI
KRYQAISEFK NEKIGACVKP CLVFNGPKWA QTEELRRLRN LFIDTFQREK VDSIRLQGIE
HVLSFTVTDD MNILMRSYRI LLKKSGQRTP RIELEEIGPS ADFSIRRTKI ASEDLYKQAR
KQPKQLKVGK KKNISTDALG NTKGRVHLGK QQTGSIQTRR VKALRKTPEE KKENRQRKKV
ALKAAAAEAL ASQGNNPFSS
