RPFA_MYCTU
ID RPFA_MYCTU Reviewed; 407 AA.
AC P9WG31; F2GIY6; L0T504; O53879; Q7D947;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 34.
DE RecName: Full=Resuscitation-promoting factor RpfA;
DE EC=3.-.-.-;
DE Flags: Precursor;
GN Name=rpfA; OrderedLocusNames=Rv0867c; ORFNames=MTV043.60c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION, AND INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=12410821; DOI=10.1046/j.1365-2958.2002.03184.x;
RA Mukamolova G.V., Turapov O.A., Young D.I., Kaprelyants A.S., Kell D.B.,
RA Young M.;
RT "A family of autocrine growth factors in Mycobacterium tuberculosis.";
RL Mol. Microbiol. 46:623-635(2002).
RN [3]
RP BIOTECHNOLOGY.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=12874362; DOI=10.1128/iai.71.8.4789-4794.2003;
RA Yeremeev V.V., Kondratieva T.K., Rubakova E.I., Petrovskaya S.N.,
RA Kazarian K.A., Telkov M.V., Biketov S.F., Kaprelyants A.S., Apt A.S.;
RT "Proteins of the Rpf family: immune cell reactivity and vaccination
RT efficacy against tuberculosis in mice.";
RL Infect. Immun. 71:4789-4794(2003).
RN [4]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=15207486; DOI=10.1016/j.tube.2003.12.004;
RA Downing K.J., Betts J.C., Young D.I., McAdam R.A., Kelly F., Young M.,
RA Mizrahi V.;
RT "Global expression profiling of strains harbouring null mutations reveals
RT that the five rpf-like genes of Mycobacterium tuberculosis show functional
RT redundancy.";
RL Tuberculosis 84:167-179(2004).
RN [5]
RP INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=15882420; DOI=10.1111/j.1365-2958.2005.04609.x;
RA Rickman L., Scott C., Hunt D.M., Hutchinson T., Menendez M.C., Whalan R.,
RA Hinds J., Colston M.J., Green J., Buxton R.S.;
RT "A member of the cAMP receptor protein family of transcription regulators
RT in Mycobacterium tuberculosis is required for virulence in mice and
RT controls transcription of the rpfA gene coding for a resuscitation
RT promoting factor.";
RL Mol. Microbiol. 56:1274-1286(2005).
RN [6]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=18186793; DOI=10.1111/j.1365-2958.2007.06078.x;
RA Kana B.D., Gordhan B.G., Downing K.J., Sung N., Vostroktunova G.,
RA Machowski E.E., Tsenova L., Young M., Kaprelyants A., Kaplan G.,
RA Mizrahi V.;
RT "The resuscitation-promoting factors of Mycobacterium tuberculosis are
RT required for virulence and resuscitation from dormancy but are collectively
RT dispensable for growth in vitro.";
RL Mol. Microbiol. 67:672-684(2008).
CC -!- FUNCTION: Factor that stimulates resuscitation of dormant cells. Has
CC peptidoglycan (PG) hydrolytic activity. Active in the pM concentration
CC range. Has little to no effect on actively-growing cells. PG fragments
CC could either directly activate the resuscitation pathway of dormant
CC bacteria or serve as a substrate for endogenous Rpf, resulting in low
CC molecular weight products with resuscitation activity. Stimulates
CC growth of stationary phase M.bovis (a slow-growing Mycobacterium),
CC reduces the lag phase of diluted fast-growers M.smegmatis and
CC Micrococcus luteus. {ECO:0000269|PubMed:12410821}.
CC -!- INDUCTION: Expressed in actively growing cells, activated by CRP.
CC {ECO:0000269|PubMed:12410821, ECO:0000269|PubMed:15882420}.
CC -!- DISRUPTION PHENOTYPE: Not essential, disruption of rpfA alone has no
CC effect on growth or survival in liquid culture, nor in mouse infection
CC models, although cells are clumpy after 2 weeks in stirred culture.
CC Alterations in gene expression are seen. All 5 genes in this family can
CC be deleted without affecting growth in culture, however triple deletion
CC mutants (rpfA-rpfC-rpfB or rpfA-rpfC-rpfD) are not able to resuscitate
CC spontaneously in the presence or absence of O(2), and are attenuated in
CC a mouse infection model. {ECO:0000269|PubMed:15207486,
CC ECO:0000269|PubMed:18186793}.
CC -!- BIOTECHNOLOGY: Might be a good vaccine candidate.
CC {ECO:0000269|PubMed:12874362}.
CC -!- SIMILARITY: Belongs to the transglycosylase family. Rpf subfamily.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP43615.1; -; Genomic_DNA.
DR PIR; C70816; C70816.
DR RefSeq; NP_215382.1; NC_000962.3.
DR RefSeq; WP_003900223.1; NZ_NVQJ01000040.1.
DR AlphaFoldDB; P9WG31; -.
DR SMR; P9WG31; -.
DR STRING; 83332.Rv0867c; -.
DR PaxDb; P9WG31; -.
DR GeneID; 885749; -.
DR KEGG; mtu:Rv0867c; -.
DR TubercuList; Rv0867c; -.
DR eggNOG; COG1652; Bacteria.
DR OMA; WPVCGGP; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005576; C:extracellular region; IDA:MTBBASE.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0010629; P:negative regulation of gene expression; IDA:MTBBASE.
DR GO; GO:0040010; P:positive regulation of growth rate; IDA:MTBBASE.
DR CDD; cd13925; RPF; 1.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR010618; RPF.
DR Pfam; PF06737; Transglycosylas; 1.
DR SUPFAM; SSF53955; SSF53955; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Reference proteome; Repeat; Signal; Virulence.
FT SIGNAL 1..33
FT /evidence="ECO:0000255"
FT CHAIN 34..407
FT /note="Resuscitation-promoting factor RpfA"
FT /id="PRO_0000421025"
FT REPEAT 178..185
FT /note="1"
FT REPEAT 186..193
FT /note="2"
FT REPEAT 218..225
FT /note="3"
FT REPEAT 226..233
FT /note="4"
FT REPEAT 240..247
FT /note="5"
FT REPEAT 248..255
FT /note="6"
FT REPEAT 274..281
FT /note="7"
FT REPEAT 287..294
FT /note="8"
FT REPEAT 295..302
FT /note="9"
FT REPEAT 303..310
FT /note="10"
FT REPEAT 311..318
FT /note="11"
FT REPEAT 353..359
FT /note="12"
FT REGION 142..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 178..359
FT /note="12 X 8 AA approximate repeats of A-P-A-D-L-A-P-P"
FT REGION 271..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..197
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 212..253
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..316
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 351..365
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 407 AA; 39975 MW; E0F0A22E4EA203CE CRC64;
MSGRHRKPTT SNVSVAKIAF TGAVLGGGGI AMAAQATAAT DGEWDQVARC ESGGNWSINT
GNGYLGGLQF TQSTWAAHGG GEFAPSAQLA SREQQIAVGE RVLATQGRGA WPVCGRGLSN
ATPREVLPAS AAMDAPLDAA AVNGEPAPLA PPPADPAPPV ELAANDLPAP LGEPLPAAPA
DPAPPADLAP PAPADVAPPV ELAVNDLPAP LGEPLPAAPA DPAPPADLAP PAPADLAPPA
PADLAPPAPA DLAPPVELAV NDLPAPLGEP LPAAPAELAP PADLAPASAD LAPPAPADLA
PPAPAELAPP APADLAPPAA VNEQTAPGDQ PATAPGGPVG LATDLELPEP DPQPADAPPP
GDVTEAPAET PQVSNIAYTK KLWQAIRAQD VCGNDALDSL AQPYVIG
