ID   RPFB_MYCS2              Reviewed;         362 AA.
AC   A0R3E0; I7GE51;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2013, sequence version 2.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Resuscitation-promoting factor RpfB;
DE            EC=3.-.-.-;
DE   Flags: Precursor;
GN   Name=rpfB; OrderedLocusNames=MSMEG_5439, MSMEI_5289;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18463693; DOI=10.1371/journal.ppat.1000001;
RA   Hett E.C., Chao M.C., Deng L.L., Rubin E.J.;
RT   "A mycobacterial enzyme essential for cell division synergizes with
RT   resuscitation-promoting factor.";
RL   PLoS Pathog. 4:E1000001-E1000001(2008).
CC   -!- FUNCTION: Factor that stimulates resuscitation of dormant cells. Has
CC       peptidoglycan (PG) hydrolytic activity. Active in the pM concentration
CC       range. Has little to no effect on actively-growing cells. PG fragments
CC       could either directly activate the resuscitation pathway of dormant
CC       bacteria or serve as a substrate for endogenous Rpf, resulting in low
CC       molecular weight products with resuscitation activity (By similarity).
CC       {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Not essential, grows normally.
CC       {ECO:0000269|PubMed:18463693}.
CC   -!- SIMILARITY: Belongs to the transglycosylase family. Rpf subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK73651.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000480; ABK73651.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP001663; AFP41731.1; -; Genomic_DNA.
DR   RefSeq; YP_889678.1; NC_008596.1.
DR   AlphaFoldDB; A0R3E0; -.
DR   SMR; A0R3E0; -.
DR   STRING; 246196.MSMEI_5289; -.
DR   CAZy; GH23; Glycoside Hydrolase Family 23.
DR   PRIDE; A0R3E0; -.
DR   EnsemblBacteria; ABK73651; ABK73651; MSMEG_5439.
DR   EnsemblBacteria; AFP41731; AFP41731; MSMEI_5289.
DR   KEGG; msg:MSMEI_5289; -.
DR   KEGG; msm:MSMEG_5439; -.
DR   PATRIC; fig|246196.19.peg.5300; -.
DR   eggNOG; COG3583; Bacteria.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016998; P:cell wall macromolecule catabolic process; IGI:UniProtKB.
DR   GO; GO:0009253; P:peptidoglycan catabolic process; IGI:UniProtKB.
DR   CDD; cd13925; RPF; 1.
DR   InterPro; IPR007137; DUF348.
DR   InterPro; IPR011098; G5_dom.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR010618; RPF.
DR   Pfam; PF03990; DUF348; 3.
DR   Pfam; PF07501; G5; 1.
DR   Pfam; PF06737; Transglycosylas; 1.
DR   SMART; SM01208; G5; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   PROSITE; PS51109; G5; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..362
FT                   /note="Resuscitation-promoting factor RpfB"
FT                   /id="PRO_0000421028"
FT   DOMAIN          192..272
FT                   /note="G5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
SQ   SEQUENCE   362 AA;  38271 MW;  B0210AA85FEC9A3A CRC64;
     MLRGVVGAFL VSLTVAGSYA VASHKTVTLS VDGAPMTVTT MKSRVIDIVE ENGFEVSDRD
     DLFPAATETV HQSDTIVLRR SRPLEVSLDG EGTKQVWTTA STVDEALAQL RMTDKAPAAA
     SRGSRVPLAG MALPVVSPKT VQIDDGGKVS TVHLAAPNVA GLLEAAGVPL EQNDKVVPAA
     SSPVVDGMQI QVTRMRIEKV TERVPLAPGN ERIEDPTMNM SRQVVEDPGA PGLQDVTFAI
     AKVNGVETGR LPVANQVIEP ARNGVLRVGA KPGTEVPPVS NGHTWDALAQ CEAGGNWAIN
     TGNGFYGGVQ FDQNTWERNG GLRYAPRADL ATREEQIAIA TVTQSRQGWG AWPVCSGRIG
     AR