ID   RPFB_MYCTO              Reviewed;         362 AA.
AC   P9WG28; F2GHD1; L0T719; O05594; Q7D900;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Resuscitation-promoting factor RpfB;
DE            EC=3.-.-.-;
DE   Flags: Precursor;
GN   Name=rpfB; OrderedLocusNames=MT1038;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Factor that stimulates resuscitation of dormant cells. Has
CC       peptidoglycan (PG) hydrolytic activity. PG fragments could either
CC       directly activate the resuscitation pathway of dormant bacteria or
CC       serve as a substrate for endogenous Rpf, resulting in low molecular
CC       weight products with resuscitation activity (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303}; Lipid-anchor {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the transglycosylase family. Rpf subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK45288.1; -; Genomic_DNA.
DR   PIR; D70603; D70603.
DR   RefSeq; WP_003405187.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WG28; -.
DR   SMR; P9WG28; -.
DR   CAZy; GH23; Glycoside Hydrolase Family 23.
DR   EnsemblBacteria; AAK45288; AAK45288; MT1038.
DR   KEGG; mtc:MT1038; -.
DR   PATRIC; fig|83331.31.peg.1113; -.
DR   HOGENOM; CLU_036884_1_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProt.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0010629; P:negative regulation of gene expression; IEA:UniProt.
DR   GO; GO:0040010; P:positive regulation of growth rate; IEA:UniProt.
DR   CDD; cd13925; RPF; 1.
DR   InterPro; IPR007137; DUF348.
DR   InterPro; IPR011098; G5_dom.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR010618; RPF.
DR   Pfam; PF03990; DUF348; 3.
DR   Pfam; PF07501; G5; 1.
DR   Pfam; PF06737; Transglycosylas; 1.
DR   SMART; SM01208; G5; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
DR   PROSITE; PS51109; G5; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Disulfide bond; Hydrolase; Lipoprotein; Membrane; Palmitate;
KW   Signal; Virulence.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           24..362
FT                   /note="Resuscitation-promoting factor RpfB"
FT                   /id="PRO_0000428434"
FT   DOMAIN          192..272
FT                   /note="G5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00437"
FT   LIPID           24
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           24
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   DISULFID        291..355
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   362 AA;  38078 MW;  02B55D8C70373D10 CRC64;
     MLRLVVGALL LVLAFAGGYA VAACKTVTLT VDGTAMRVTT MKSRVIDIVE ENGFSVDDRD
     DLYPAAGVQV HDADTIVLRR SRPLQISLDG HDAKQVWTTA STVDEALAQL AMTDTAPAAA
     SRASRVPLSG MALPVVSAKT VQLNDGGLVR TVHLPAPNVA GLLSAAGVPL LQSDHVVPAA
     TAPIVEGMQI QVTRNRIKKV TERLPLPPNA RRVEDPEMNM SREVVEDPGV PGTQDVTFAV
     AEVNGVETGR LPVANVVVTP AHEAVVRVGT KPGTEVPPVI DGSIWDAIAG CEAGGNWAIN
     TGNGYYGGVQ FDQGTWEANG GLRYAPRADL ATREEQIAVA EVTRLRQGWG AWPVCAARAG
     AR