ID   RPFD_MYCTU              Reviewed;         154 AA.
AC   P9WG27; F2GI59; L0TC67; P71755; Q7D783;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Resuscitation-promoting factor RpfD;
DE            EC=3.-.-.-;
GN   Name=rpfD; OrderedLocusNames=Rv2389c; ORFNames=MTCY253.32;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12410821; DOI=10.1046/j.1365-2958.2002.03184.x;
RA   Mukamolova G.V., Turapov O.A., Young D.I., Kaprelyants A.S., Kell D.B.,
RA   Young M.;
RT   "A family of autocrine growth factors in Mycobacterium tuberculosis.";
RL   Mol. Microbiol. 46:623-635(2002).
RN   [3]
RP   BIOTECHNOLOGY.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12874362; DOI=10.1128/iai.71.8.4789-4794.2003;
RA   Yeremeev V.V., Kondratieva T.K., Rubakova E.I., Petrovskaya S.N.,
RA   Kazarian K.A., Telkov M.V., Biketov S.F., Kaprelyants A.S., Apt A.S.;
RT   "Proteins of the Rpf family: immune cell reactivity and vaccination
RT   efficacy against tuberculosis in mice.";
RL   Infect. Immun. 71:4789-4794(2003).
RN   [4]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15207486; DOI=10.1016/j.tube.2003.12.004;
RA   Downing K.J., Betts J.C., Young D.I., McAdam R.A., Kelly F., Young M.,
RA   Mizrahi V.;
RT   "Global expression profiling of strains harbouring null mutations reveals
RT   that the five rpf-like genes of Mycobacterium tuberculosis show functional
RT   redundancy.";
RL   Tuberculosis 84:167-179(2004).
RN   [5]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=18186793; DOI=10.1111/j.1365-2958.2007.06078.x;
RA   Kana B.D., Gordhan B.G., Downing K.J., Sung N., Vostroktunova G.,
RA   Machowski E.E., Tsenova L., Young M., Kaprelyants A., Kaplan G.,
RA   Mizrahi V.;
RT   "The resuscitation-promoting factors of Mycobacterium tuberculosis are
RT   required for virulence and resuscitation from dormancy but are collectively
RT   dispensable for growth in vitro.";
RL   Mol. Microbiol. 67:672-684(2008).
CC   -!- FUNCTION: Factor that stimulates resuscitation of dormant cells. Has
CC       peptidoglycan (PG) hydrolytic activity. Active in the pM concentration
CC       range. Has little to no effect on actively-growing cells. PG fragments
CC       could either directly activate the resuscitation pathway of dormant
CC       bacteria or serve as a substrate for endogenous Rpf, resulting in low
CC       molecular weight products with resuscitation activity.
CC       {ECO:0000269|PubMed:12410821}.
CC   -!- FUNCTION: Stimulates growth of stationary phase M.bovis (a slow-growing
CC       Mycobacterium), reduces the lag phase of diluted fast-growers
CC       M.smegmatis and Micrococcus luteus. Sequential gene disruption
CC       indicates RpfB and RpfE are higher than RpfD and RpfC in functional
CC       hierarchy. {ECO:0000269|PubMed:12410821}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Expressed in actively growing cells.
CC       {ECO:0000269|PubMed:12410821}.
CC   -!- DISRUPTION PHENOTYPE: Not essential, disruption of rpfC alone has no
CC       effect on growth or survival in liquid culture, nor in mouse infection
CC       models,although colony size is reduced. Alterations in gene expression
CC       are seen. All 5 genes in this family can be deleted without affecting
CC       growth in culture, however triple deletion mutants (rpfA-rpfC-rpfB or
CC       rpfA-rpfC-rpfD) are not able to resuscitate spontaneously in the
CC       presence or absence of O(2), and are attenuated in a mouse infection
CC       model. {ECO:0000269|PubMed:15207486, ECO:0000269|PubMed:18186793}.
CC   -!- BIOTECHNOLOGY: Might be a good vaccine candidate.
CC       {ECO:0000269|PubMed:12874362}.
CC   -!- SIMILARITY: Belongs to the transglycosylase family. Rpf subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP45177.1; -; Genomic_DNA.
DR   PIR; H70681; H70681.
DR   RefSeq; NP_216905.1; NC_000962.3.
DR   RefSeq; WP_003412293.1; NZ_NVQJ01000029.1.
DR   AlphaFoldDB; P9WG27; -.
DR   SMR; P9WG27; -.
DR   STRING; 83332.Rv2389c; -.
DR   PaxDb; P9WG27; -.
DR   GeneID; 885246; -.
DR   KEGG; mtu:Rv2389c; -.
DR   TubercuList; Rv2389c; -.
DR   eggNOG; COG1388; Bacteria.
DR   OMA; PSTWAEH; -.
DR   PhylomeDB; P9WG27; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IDA:MTBBASE.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0085016; P:dormancy exit of symbiont in host; IDA:MTBBASE.
DR   GO; GO:0010629; P:negative regulation of gene expression; IDA:MTBBASE.
DR   GO; GO:0010628; P:positive regulation of gene expression; IDA:MTBBASE.
DR   GO; GO:0040010; P:positive regulation of growth rate; IDA:MTBBASE.
DR   CDD; cd13925; RPF; 1.
DR   InterPro; IPR023346; Lysozyme-like_dom_sf.
DR   InterPro; IPR010618; RPF.
DR   Pfam; PF06737; Transglycosylas; 1.
DR   SUPFAM; SSF53955; SSF53955; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Hydrolase; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..154
FT                   /note="Resuscitation-promoting factor RpfD"
FT                   /id="PRO_0000421030"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   154 AA;  15679 MW;  27AFD997D193DFE7 CRC64;
     MTPGLLTTAG AGRPRDRCAR IVCTVFIETA VVATMFVALL GLSTISSKAD DIDWDAIAQC
     ESGGNWAANT GNGLYGGLQI SQATWDSNGG VGSPAAASPQ QQIEVADNIM KTQGPGAWPK
     CSSCSQGDAP LGSLTHILTF LAAETGGCSG SRDD