RPGF1_CAEEL
ID RPGF1_CAEEL Reviewed; 1038 AA.
AC P34578;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 3.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Rap guanine nucleotide exchange factor 1;
DE AltName: Full=Exchange protein activated by cyclic AMP 1;
GN Name=epac-1; ORFNames=T20G5.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, INTERACTION WITH DRN-1, AND TISSUE SPECIFICITY.
RX PubMed=22897658; DOI=10.1111/j.1365-2443.2012.01627.x;
RA Tada M., Gengyo-Ando K., Kobayashi T., Fukuyama M., Mitani S., Kontani K.,
RA Katada T.;
RT "Neuronally expressed Ras-family GTPase Di-Ras modulates synaptic activity
RT in Caenorhabditis elegans.";
RL Genes Cells 17:778-789(2012).
CC -!- FUNCTION: Guanine nucleotide-releasing protein (By similarity).
CC Together with GTPase drn-1, may regulate acetylcholine release at the
CC neuromuscular junctions probably downstream of G-protein gsa-1 and
CC adenylate cyclase acy-1 (PubMed:22897658).
CC {ECO:0000250|UniProtKB:Q13905, ECO:0000269|PubMed:22897658}.
CC -!- SUBUNIT: Interacts (via C-terminus) with drn-1.
CC {ECO:0000269|PubMed:22897658}.
CC -!- TISSUE SPECIFICITY: Expressed specifically in neurons including the
CC nerve ring, ventral and dorsal nerve cord motor neurons and tail
CC ganglia. {ECO:0000269|PubMed:22897658}.
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DR EMBL; Z30423; CAA83013.3; -; Genomic_DNA.
DR PIR; S42368; S42368.
DR RefSeq; NP_499256.2; NM_066855.3.
DR AlphaFoldDB; P34578; -.
DR SMR; P34578; -.
DR BioGRID; 41626; 1.
DR STRING; 6239.T20G5.5; -.
DR PaxDb; P34578; -.
DR EnsemblMetazoa; T20G5.5.1; T20G5.5.1; WBGene00004255.
DR GeneID; 176432; -.
DR KEGG; cel:CELE_T20G5.5; -.
DR CTD; 176432; -.
DR WormBase; T20G5.5; CE43630; WBGene00004255; epac-1.
DR eggNOG; KOG2378; Eukaryota.
DR GeneTree; ENSGT00940000172554; -.
DR HOGENOM; CLU_006829_1_0_1; -.
DR InParanoid; P34578; -.
DR OMA; CMHEHEL; -.
DR OrthoDB; 143470at2759; -.
DR PhylomeDB; P34578; -.
DR Reactome; R-CEL-354192; Integrin signaling.
DR Reactome; R-CEL-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-CEL-392517; Rap1 signalling.
DR PRO; PR:P34578; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00004255; Expressed in pharyngeal muscle cell (C elegans) and 2 other tissues.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00038; CAP_ED; 2.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00027; cNMP_binding; 2.
DR Pfam; PF00610; DEP; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00100; cNMP; 2.
DR SMART; SM00049; DEP; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR SUPFAM; SSF51206; SSF51206; 2.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 2.
DR PROSITE; PS50186; DEP; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 1: Evidence at protein level;
KW Guanine-nucleotide releasing factor; Reference proteome; Repeat.
FT CHAIN 1..1038
FT /note="Rap guanine nucleotide exchange factor 1"
FT /id="PRO_0000068900"
FT DOMAIN 234..316
FT /note="DEP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00066"
FT DOMAIN 516..654
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 795..1028
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT BINDING 10..140
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /ligand_label="1"
FT BINDING 375..492
FT /ligand="a nucleoside 3',5'-cyclic phosphate"
FT /ligand_id="ChEBI:CHEBI:58464"
FT /ligand_label="2"
SQ SEQUENCE 1038 AA; 118871 MW; E8356D9D44E05819 CRC64;
MERIVSRVRR LSPLHTFSDA LLISLLSESD FQPDSIQQGV VLFEKDEPTE YWYLLLSGEV
QLYSKTYTGD FNHLKTLRCG ALFGDLSTLT HSCSCLVTRP AQLIRIAQNH FLSVYNKHGD
HLQPFIIIMH DILTDETPSD PIHPHSSGLF NGQRSMDLIS TEINPSEIVS VSTNGMLSKM
ILPSIPNQRE KPMNRVVVNQ QKNEERNFIE FHNPTGIEKQ IRDSGGILHR KMLTDNHQVI
RDITTEHTRV QNCMIGAEMI DWLLTLFVST STTCSSLSRI QMSAIWQVLL NNGLISHIDG
EHQFLDKTNS YYRWVQQFRS RNKVAPSIEE VSKSITLLSS VAPETLFLMI VSKPGFERSP
EELEVVYEEL TFIKALSHLS TMVKRQLSNF VKVEQYVHAG SVVFRQGEIG VYWYIVLKGA
VEVNVNGKIV CLLREGDDFG KLALVNDLPR AATIVTYEDD SMFLVVDKHH FNQILHQVEA
NTVRLKDYGE DVLVLEKVDI PRGAALENSN SCNFNCGYSV MAGKAEKILE YVLETRIDAL
GDDISELDVF VEDFILTHDA FMPDNTVCNF LKSYYFRTPY RATRDSITDS CTEEVRCKRR
VVQFVYVWCS LLRVNFFLNP VTNSFVEELF CHVIDDRKRL GGMEDILTRI GSIRSTRENM
QLVLARHPAI VLDCGVLSAH TPCPVLPSDV CNQIIYLADT TCFVLPIRVD KTAEEICELS
RRRMSFSAEP LNLVEVKSNG EKLIFSPNDR AIPTVLSLNS KLYVVNREEI PLLVPMEDQN
GPTPSSHSSI LHLIDSQELA HQLFLFHLQL LRSTDSNELL YQVIGRESFP LSMPFNLDLL
VRRFNEVQHW STTEILLATE ENRMEILKKF ISIATIAREY RDLLTVFAIT LGLSHTSISR
LTLTWSKLPP ASLKTFSELE NLLDPTRNHR MYRLLVSKMS SPYIPFVPLI LKDLMFIHQG
NKSFYNGLVN FEKMHMFAKI FRSFRQCKSQ MDNGAEHEFI EPQSLIRNLR VIDNQKKLMQ
LSYEIEPKSA PKRNVIFH
