ID   RPGF5_RAT               Reviewed;         580 AA.
AC   P83900;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2004, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Rap guanine nucleotide exchange factor 5;
DE   AltName: Full=M-Ras-regulated Rap GEF;
DE            Short=MR-GEF;
DE            Short=rMR-GEF;
GN   Name=Rapgef5; Synonyms=Mrgef;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116 {ECO:0000312|EMBL:AAR83745.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP   INDUCTION.
RC   STRAIN=Sprague-Dawley {ECO:0000312|EMBL:AAR83745.1};
RC   TISSUE=Brain cortex {ECO:0000312|EMBL:AAR83745.1};
RX   PubMed=14643017; DOI=10.1016/j.devbrainres.2003.09.017;
RA   Bithell A., Alberta J., Hornby F., Stiles C.D., Williams B.P.;
RT   "Expression of the guanine nucleotide exchange factor, mr-gef, is regulated
RT   during the differentiation of specific subsets of telencephalic neurons.";
RL   Brain Res. Dev. Brain Res. 146:107-118(2003).
CC   -!- FUNCTION: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and
CC       MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: In the embryo, expressed in young neurons of the
CC       developing telencephalon, diencephalon and hindbrain. Not expressed in
CC       progenitor cells in the ventricular zone.
CC       {ECO:0000269|PubMed:14643017}.
CC   -!- DEVELOPMENTAL STAGE: Expression is first detected at embryonic day 13.
CC       {ECO:0000269|PubMed:14643017}.
CC   -!- INDUCTION: Down-regulated in nerve growth factor-treated PC12 cells.
CC       {ECO:0000269|PubMed:14643017}.
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DR   EMBL; AY390379; AAR83745.1; -; mRNA.
DR   RefSeq; NP_001041380.1; NM_001047915.1.
DR   AlphaFoldDB; P83900; -.
DR   SMR; P83900; -.
DR   BioGRID; 263730; 1.
DR   STRING; 10116.ENSRNOP00000007112; -.
DR   iPTMnet; P83900; -.
DR   PhosphoSitePlus; P83900; -.
DR   PaxDb; P83900; -.
DR   PRIDE; P83900; -.
DR   GeneID; 362799; -.
DR   KEGG; rno:362799; -.
DR   UCSC; RGD:1561668; rat.
DR   CTD; 9771; -.
DR   RGD; 1561668; Rapgef5.
DR   eggNOG; KOG2378; Eukaryota.
DR   InParanoid; P83900; -.
DR   OrthoDB; 143470at2759; -.
DR   PhylomeDB; P83900; -.
DR   PRO; PR:P83900; -.
DR   Proteomes; UP000002494; Unplaced.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0030742; F:GTP-dependent protein binding; ISO:RGD.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISO:RGD.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS00720; RASGEF; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   2: Evidence at transcript level;
KW   Guanine-nucleotide releasing factor; Nucleus; Reference proteome.
FT   CHAIN           1..580
FT                   /note="Rap guanine nucleotide exchange factor 5"
FT                   /id="PRO_0000068875"
FT   DOMAIN          67..200
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135,
FT                   ECO:0000305"
FT   DOMAIN          344..579
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168,
FT                   ECO:0000305"
SQ   SEQUENCE   580 AA;  67601 MW;  9B3A0B49944E40FC CRC64;
     MGSSRLRVFD PPLERKDSAA LSERQLPLPT FDVPYFKYID EEDEDDEWGS RSQSSTEDDS
     VDSLLSDRYV VVSGTPEKIL EHLLNDLHLA EVQHKETETL LDDFLLTYTV FMTTDDLCQA
     LLRHYSAKKY QGEEENSDVP CRKRKVLHLV SQWISLYKDW LHEDEHSKMF LKTIYRNVLD
     DVYEYPILEK ELKEFQKILG MHRRHTVDEY SPQKKNKALF HQFSLKENWL QHRGTVAETE
     EIFCHVYVTE HSYISVKAKV SSTAQEILKV VAEKLQRAEE DLALVAIMFS GEKHEFQPND
     LAISKSLEAS GRIYVYRKDL ADTLNPFAEN EESQQRSMRI LGMNTWDLAL ELMSFDWSLF
     NSIHEQELIY FTFSRQGSGE HTVNLSLLLQ RCNEVQLWVA TEILLCSQLG KRVQLVKKFI
     KIAAHCKAQQ NLNSFFAIVM GLNTASVSRL SQTWEKIPGK FKKLFSELES LTDPSLNHKA
     YRDAFKKMKP PKIPFMPLLL KDVTFIHEGN KTFLDNLVNF EKLHMIADTV RTLRHCRTNQ
     FGSDMSPKEQ QELKSYVNHL YVIDSQQALF ELSHRLEPRA