RPH1_ARATH
ID RPH1_ARATH Reviewed; 197 AA.
AC Q9ZU82; Q94AU8;
DT 07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Protein RESISTANCE TO PHYTOPHTHORA 1, chloroplastic {ECO:0000303|PubMed:19170932};
DE Short=AtRPH1 {ECO:0000303|PubMed:19170932};
DE Flags: Precursor;
GN Name=RPH1 {ECO:0000303|PubMed:19170932};
GN OrderedLocusNames=At2g48070 {ECO:0000312|Araport:AT2G48070};
GN ORFNames=T9J23.22 {ECO:0000312|EMBL:AAD13710.2};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=19170932; DOI=10.1111/j.1365-313x.2008.03779.x;
RA Belhaj K., Lin B., Mauch F.;
RT "The chloroplast protein RPH1 plays a role in the immune response of
RT Arabidopsis to Phytophthora brassicae.";
RL Plant J. 58:287-298(2009).
CC -!- FUNCTION: Plays a positive role in the immune response to the oomycetes
CC P.brassicae, including induced oxidative burst (e.g. H(2)O(2)) and
CC enhanced expression of defense-related genes.
CC {ECO:0000269|PubMed:19170932}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:19170932}. Membrane {ECO:0000255}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Small plants with reduced chlorophyll content.
CC Susceptiblility to the oomycete pathogen P.brassicae despite
CC recognition and rapid induction of hypersensitive response (HR) but
CC associated with a reduced oxidative burst (e.g. H(2)O(2)), a runaway
CC cell-death response, and specific deficiencies in the expression of
CC established markers of immune and stress hormone signaling (e.g. PR1,
CC PDF1.2, AIG1, EDS1 and PAD4). Normal susceptibility to virulent and
CC avirulent isolates of the oomycete H.arabidopsis, to virulent and
CC avirulent isolates of the bacterial pathogen P.syringae, and to the
CC fungal pathogen B.cinerea. {ECO:0000269|PubMed:19170932}.
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DR EMBL; AC006072; AAD13710.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10928.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10929.1; -; Genomic_DNA.
DR EMBL; AY045783; AAK76457.1; -; mRNA.
DR EMBL; AY079358; AAL85089.1; -; mRNA.
DR PIR; A84923; A84923.
DR RefSeq; NP_566122.1; NM_130374.4.
DR RefSeq; NP_850484.1; NM_180153.2.
DR AlphaFoldDB; Q9ZU82; -.
DR IntAct; Q9ZU82; 1.
DR STRING; 3702.AT2G48070.3; -.
DR PRIDE; Q9ZU82; -.
DR ProteomicsDB; 227963; -.
DR EnsemblPlants; AT2G48070.1; AT2G48070.1; AT2G48070.
DR EnsemblPlants; AT2G48070.2; AT2G48070.2; AT2G48070.
DR GeneID; 819419; -.
DR Gramene; AT2G48070.1; AT2G48070.1; AT2G48070.
DR Gramene; AT2G48070.2; AT2G48070.2; AT2G48070.
DR KEGG; ath:AT2G48070; -.
DR Araport; AT2G48070; -.
DR HOGENOM; CLU_106462_0_0_1; -.
DR PhylomeDB; Q9ZU82; -.
DR PRO; PR:Q9ZU82; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZU82; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0050665; P:hydrogen peroxide biosynthetic process; IMP:UniProtKB.
DR GO; GO:1902290; P:positive regulation of defense response to oomycetes; IMP:UniProtKB.
DR InterPro; IPR044966; RPH1.
DR PANTHER; PTHR36359; PTHR36359; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plant defense; Plastid; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 53..197
FT /note="Protein RESISTANCE TO PHYTOPHTHORA 1, chloroplastic"
FT /id="PRO_0000445599"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 35..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 197 AA; 22094 MW; 5363C1356CE31D76 CRC64;
MSWSLCSTHG VSSSIALTYG FRHRRRSTFR IFATSDGLEP KDDPPESPLP SSSSALGKDL
KKVVNKTAAT FAPRASTASK NPALPGTTLY KVFEVQGYAS MFLGGVLSFN LLFPSSEPDL
WRLMGMWSIW MFTIPSLRAR DCPSKEKEAL NYLFLIVPLL NVAIPFFWKS FALVWSADTV
AFFAMYAWKL GWLERTE
