RPI1_YEAST
ID RPI1_YEAST Reviewed; 407 AA.
AC P23250; D6VVG8; E9P8U9;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Negative RAS protein regulator protein;
GN Name=RPI1; OrderedLocusNames=YIL119C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1649384; DOI=10.1128/mcb.11.8.3894-3904.1991;
RA Kim J.-H., Powers S.;
RT "Overexpression of RPI1, a novel inhibitor of the yeast Ras-cyclic AMP
RT pathway, down-regulates normal but not mutationally activated ras
RT function.";
RL Mol. Cell. Biol. 11:3894-3904(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Negative regulator of the Ras-cyclic AMP pathway. Negatively
CC regulate the activity of normal but not mutationally activated Ras
CC proteins. The down-regulatory effect of RPI1 requires the presence of
CC one of the two Ras GTPase activators, IRA1 and IRA2.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1340 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M63178; AAA35001.1; -; Genomic_DNA.
DR EMBL; S42682; AAB39843.1; -; Genomic_DNA.
DR EMBL; Z46833; CAA86873.1; -; Genomic_DNA.
DR EMBL; AY558088; AAS56414.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08434.1; -; Genomic_DNA.
DR PIR; S49890; S49890.
DR RefSeq; NP_012147.3; NM_001179467.3.
DR AlphaFoldDB; P23250; -.
DR BioGRID; 34872; 75.
DR DIP; DIP-3802N; -.
DR IntAct; P23250; 3.
DR STRING; 4932.YIL119C; -.
DR iPTMnet; P23250; -.
DR MaxQB; P23250; -.
DR PaxDb; P23250; -.
DR PRIDE; P23250; -.
DR EnsemblFungi; YIL119C_mRNA; YIL119C; YIL119C.
DR GeneID; 854687; -.
DR KEGG; sce:YIL119C; -.
DR SGD; S000001381; RPI1.
DR VEuPathDB; FungiDB:YIL119C; -.
DR eggNOG; ENOG502S5B4; Eukaryota.
DR HOGENOM; CLU_702393_0_0_1; -.
DR InParanoid; P23250; -.
DR OMA; HENEQNR; -.
DR BioCyc; YEAST:G3O-31372-MON; -.
DR PRO; PR:P23250; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P23250; protein.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030695; F:GTPase regulator activity; IMP:SGD.
DR GO; GO:0009272; P:fungal-type cell wall biogenesis; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0007265; P:Ras protein signal transduction; IMP:SGD.
DR InterPro; IPR001005; SANT/Myb.
DR PROSITE; PS50090; MYB_LIKE; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..407
FT /note="Negative RAS protein regulator protein"
FT /id="PRO_0000197113"
FT DOMAIN 90..158
FT /note="Myb-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT REGION 51..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..273
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..92
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 37
FT /note="N -> K (in Ref. 1; AAA35001/AAB39843)"
FT /evidence="ECO:0000305"
FT CONFLICT 71..73
FT /note="SNS -> P (in Ref. 1; AAA35001/AAB39843)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="A -> V (in Ref. 1; AAA35001/AAB39843)"
FT /evidence="ECO:0000305"
FT CONFLICT 170..174
FT /note="Missing (in Ref. 1; AAA35001/AAB39843)"
FT /evidence="ECO:0000305"
FT CONFLICT 190
FT /note="S -> P (in Ref. 4; AAS56414)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="C -> S (in Ref. 1; AAA35001/AAB39843)"
FT /evidence="ECO:0000305"
FT CONFLICT 242
FT /note="N -> S (in Ref. 1; AAA35001/AAB39843)"
FT /evidence="ECO:0000305"
FT CONFLICT 252
FT /note="N -> NNNNSNN (in Ref. 1; AAA35001/AAB39843)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 407 AA; 46623 MW; C6A18DCD04F58701 CRC64;
MYLEYLQPKL NLMDESSTIS KNFPDYSPNL NTPITSNFNE ETGSDCSLVT PRIISSSNSN
SNSNSNSNSN SNSGSIDENE LNNSNSSSSS ARQIRKKWKE PEDIAFITTI MNNSQLLTFV
EYFKPMKNFW KKISKILFQQ YGYERNSRQC HDRFKVLYTK SLKVHPSKKS KQKKKKSKQE
AGSNLNFDPS KLSRMQYLLV QLQNTFSFVN GNIILKSQKT LKPNKNGTND NINNHYYNNC
NNNNNNINNS NNSNNNNSNN INRNSNHSTN VFSTPEHIQS SINLDKLESL PALDTKGEPS
FISPAQFSLL SSAPADNLIL QTPPSPFFQQ TMPIQLPRDA QQEQISPVFS TDVIYMWQTM
FNTIENLKEQ VNCLKNEVKQ LNHKFYQQNK PLHNMSTSDS ENFMQQH
