ID   RPI4_ARATH              Reviewed;         296 AA.
AC   Q9FI13;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Probable ribose-5-phosphate isomerase 4, chloroplastic;
DE            EC=5.3.1.6;
DE   AltName: Full=Phosphoriboisomerase 4;
DE   Flags: Precursor;
GN   Name=RPI4; OrderedLocusNames=At5g44520; ORFNames=MFC16.20;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT   features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT   clones.";
RL   DNA Res. 6:183-195(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=19744161; DOI=10.1111/j.1399-3054.2009.01276.x;
RA   Xiong Y., DeFraia C., Williams D., Zhang X., Mou Z.;
RT   "Deficiency in a cytosolic ribose-5-phosphate isomerase causes chloroplast
RT   dysfunction, late flowering and premature cell death in Arabidopsis.";
RL   Physiol. Plantarum 137:249-263(2009).
CC   -!- FUNCTION: Catalyzes the reversible conversion of ribose-5-phosphate to
CC       ribulose 5-phosphate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate;
CC         Xref=Rhea:RHEA:14657, ChEBI:CHEBI:58121, ChEBI:CHEBI:58273;
CC         EC=5.3.1.6;
CC   -!- PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-ribose
CC       5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step
CC       1/1.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC       conditions. {ECO:0000269|PubMed:19744161}.
CC   -!- SIMILARITY: Belongs to the ribose 5-phosphate isomerase family.
CC       {ECO:0000305}.
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DR   EMBL; AB017065; BAB09159.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED95119.1; -; Genomic_DNA.
DR   EMBL; AY065286; AAL38762.1; -; mRNA.
DR   EMBL; AY133736; AAM91670.1; -; mRNA.
DR   EMBL; AK221659; BAD95333.1; -; mRNA.
DR   EMBL; AY087134; AAM64692.1; -; mRNA.
DR   RefSeq; NP_199265.1; NM_123819.4.
DR   AlphaFoldDB; Q9FI13; -.
DR   SMR; Q9FI13; -.
DR   BioGRID; 19729; 2.
DR   IntAct; Q9FI13; 2.
DR   STRING; 3702.AT5G44520.1; -.
DR   PaxDb; Q9FI13; -.
DR   PRIDE; Q9FI13; -.
DR   ProteomicsDB; 228205; -.
DR   EnsemblPlants; AT5G44520.1; AT5G44520.1; AT5G44520.
DR   GeneID; 834479; -.
DR   Gramene; AT5G44520.1; AT5G44520.1; AT5G44520.
DR   KEGG; ath:AT5G44520; -.
DR   Araport; AT5G44520; -.
DR   TAIR; locus:2163436; AT5G44520.
DR   eggNOG; KOG3075; Eukaryota.
DR   HOGENOM; CLU_056590_2_0_1; -.
DR   InParanoid; Q9FI13; -.
DR   OMA; FPCKAIV; -.
DR   PhylomeDB; Q9FI13; -.
DR   UniPathway; UPA00115; UER00412.
DR   PRO; PR:Q9FI13; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FI13; baseline and differential.
DR   Genevisible; Q9FI13; AT.
DR   GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR   GO; GO:0004751; F:ribose-5-phosphate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009052; P:pentose-phosphate shunt, non-oxidative branch; IEA:InterPro.
DR   CDD; cd01398; RPI_A; 1.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   InterPro; IPR004788; Ribose5P_isomerase_type_A.
DR   Pfam; PF06026; Rib_5-P_isom_A; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Isomerase; Plastid; Reference proteome; Transit peptide.
FT   TRANSIT         1..30
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           31..296
FT                   /note="Probable ribose-5-phosphate isomerase 4,
FT                   chloroplastic"
FT                   /id="PRO_0000425981"
SQ   SEQUENCE   296 AA;  32163 MW;  9559E8B7EA1C83AC CRC64;
     MVTATASTPF SLSSSVVFTR RRNFKRFPVC ASLSPEVSPL LRAAHHTVDN YVKSGMIIGL
     GSGEASDFAI RYLGQQLGSG SLHNVVGVPM SARSASEAAK YGIPLEYYRD GVQIDFAFHD
     ADAVEENTLI AVIGRRRSSQ EDDYILKQKS IVKVADEAVF MIKEEQYKAG LEGSIPVLVQ
     SLNWLAIAEE IDDLYLGDAE VWRRASVENE GPLGGDFPIV TSDGHNILDV IFTTPIRSLA
     DLATSLDKID GVVDHGLIIK TRCTVVIAEE TVVRSVTLQT SALEGGVRTY GQTLQK