RPIA_METJA
ID RPIA_METJA Reviewed; 226 AA.
AC Q58998;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Ribose-5-phosphate isomerase A {ECO:0000255|HAMAP-Rule:MF_00170};
DE EC=5.3.1.6 {ECO:0000255|HAMAP-Rule:MF_00170};
DE AltName: Full=Phosphoriboisomerase A {ECO:0000255|HAMAP-Rule:MF_00170};
DE Short=PRI {ECO:0000255|HAMAP-Rule:MF_00170};
GN Name=rpiA {ECO:0000255|HAMAP-Rule:MF_00170}; OrderedLocusNames=MJ1603;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP PATHWAY.
RX PubMed=16237021; DOI=10.1128/jb.187.21.7382-7389.2005;
RA Grochowski L.L., Xu H., White R.H.;
RT "Ribose-5-phosphate biosynthesis in Methanocaldococcus jannaschii occurs in
RT the absence of a pentose-phosphate pathway.";
RL J. Bacteriol. 187:7382-7389(2005).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS,
RP ACTIVE SITE, AND SUBUNIT.
RX PubMed=20054114; DOI=10.1107/s1744309109044923;
RA Strange R.W., Antonyuk S.V., Ellis M.J., Bessho Y., Kuramitsu S.,
RA Yokoyama S., Hasnain S.S.;
RT "The structure of an archaeal ribose-5-phosphate isomerase from
RT Methanocaldococcus jannaschii (MJ1603).";
RL Acta Crystallogr. F 65:1214-1217(2009).
CC -!- FUNCTION: Catalyzes the reversible conversion of ribose-5-phosphate to
CC ribulose 5-phosphate. {ECO:0000255|HAMAP-Rule:MF_00170}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate;
CC Xref=Rhea:RHEA:14657, ChEBI:CHEBI:58121, ChEBI:CHEBI:58273;
CC EC=5.3.1.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00170};
CC -!- PATHWAY: Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.
CC {ECO:0000269|PubMed:16237021}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:20054114}.
CC -!- SIMILARITY: Belongs to the ribose 5-phosphate isomerase family.
CC {ECO:0000255|HAMAP-Rule:MF_00170}.
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DR EMBL; L77117; AAB99623.1; -; Genomic_DNA.
DR PIR; B64500; B64500.
DR RefSeq; WP_010871128.1; NC_000909.1.
DR PDB; 3IXQ; X-ray; 1.78 A; A/B/C/D=1-226.
DR PDBsum; 3IXQ; -.
DR AlphaFoldDB; Q58998; -.
DR SMR; Q58998; -.
DR STRING; 243232.MJ_1603; -.
DR EnsemblBacteria; AAB99623; AAB99623; MJ_1603.
DR GeneID; 1452512; -.
DR KEGG; mja:MJ_1603; -.
DR eggNOG; arCOG01122; Archaea.
DR HOGENOM; CLU_056590_1_1_2; -.
DR InParanoid; Q58998; -.
DR OMA; YDWDEVN; -.
DR OrthoDB; 98657at2157; -.
DR PhylomeDB; Q58998; -.
DR BRENDA; 5.3.1.6; 3260.
DR UniPathway; UPA00293; -.
DR EvolutionaryTrace; Q58998; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004751; F:ribose-5-phosphate isomerase activity; IBA:GO_Central.
DR GO; GO:0006014; P:D-ribose metabolic process; IBA:GO_Central.
DR GO; GO:0009052; P:pentose-phosphate shunt, non-oxidative branch; IDA:UniProtKB.
DR CDD; cd01398; RPI_A; 1.
DR HAMAP; MF_00170; Rib_5P_isom_A; 1.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR InterPro; IPR020672; Ribose5P_isomerase_typA_subgr.
DR InterPro; IPR004788; Ribose5P_isomerase_type_A.
DR PANTHER; PTHR11934; PTHR11934; 1.
DR Pfam; PF06026; Rib_5-P_isom_A; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR TIGRFAMs; TIGR00021; rpiA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Isomerase; Reference proteome.
FT CHAIN 1..226
FT /note="Ribose-5-phosphate isomerase A"
FT /id="PRO_0000158510"
FT ACT_SITE 106
FT /note="Proton acceptor"
FT /evidence="ECO:0000305|PubMed:20054114"
FT BINDING 28..31
FT /ligand="substrate"
FT BINDING 83..86
FT /ligand="substrate"
FT /evidence="ECO:0000305"
FT BINDING 97..100
FT /ligand="substrate"
FT /evidence="ECO:0000305"
FT BINDING 124
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00170"
FT HELIX 4..14
FT /evidence="ECO:0007829|PDB:3IXQ"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 23..26
FT /evidence="ECO:0007829|PDB:3IXQ"
FT HELIX 30..46
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 51..56
FT /evidence="ECO:0007829|PDB:3IXQ"
FT HELIX 57..65
FT /evidence="ECO:0007829|PDB:3IXQ"
FT TURN 73..75
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 79..83
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 86..89
FT /evidence="ECO:0007829|PDB:3IXQ"
FT TURN 90..92
FT /evidence="ECO:0007829|PDB:3IXQ"
FT HELIX 103..112
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 113..121
FT /evidence="ECO:0007829|PDB:3IXQ"
FT HELIX 122..124
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 125..128
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 131..133
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 135..139
FT /evidence="ECO:0007829|PDB:3IXQ"
FT HELIX 141..143
FT /evidence="ECO:0007829|PDB:3IXQ"
FT HELIX 144..153
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 157..160
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 164..169
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 177..182
FT /evidence="ECO:0007829|PDB:3IXQ"
FT HELIX 188..196
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 201..207
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 211..217
FT /evidence="ECO:0007829|PDB:3IXQ"
FT STRAND 220..224
FT /evidence="ECO:0007829|PDB:3IXQ"
SQ SEQUENCE 226 AA; 24830 MW; C0F1C2C1A5499123 CRC64;
MSNEDLKLKV AKEAVKLVKD GMVIGLGTGS TAALFIRELG NRIREEELTV FGIPTSFEAK
MLAMQYEIPL VTLDEYDVDI AFDGADEVEE TTLFLIKGGG GCHTQEKIVD YNANEFVVLV
DESKLVKKLG EKFPIPVEVI PSAYRVVIRA LSEMGGEAVI RLGDRKRGPV ITDNGNMIID
VFMNIDDAIE LEKEINNIPG VVENGIFTKV DKVLVGTKKG VKTLKK
