RPKL1_HUMAN
ID RPKL1_HUMAN Reviewed; 549 AA.
AC Q9Y6S9; A6NGM9; Q69YT9; Q6ZMQ6; Q96NR9; Q9BSU9;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Ribosomal protein S6 kinase-like 1;
DE EC=2.7.11.1;
GN Name=RPS6KL1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12508121; DOI=10.1038/nature01348;
RA Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C.,
RA Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A.,
RA Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H.,
RA Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T.,
RA Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B.,
RA Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D.,
RA Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R.,
RA Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S.,
RA Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C.,
RA Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S.,
RA Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C.,
RA Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P.,
RA Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M.,
RA Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V.,
RA Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J.,
RA Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F.,
RA Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F.,
RA Waterston R., Hood L., Weissenbach J.;
RT "The DNA sequence and analysis of human chromosome 14.";
RL Nature 421:601-607(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 507-549.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [5]
RP VARIANTS [LARGE SCALE ANALYSIS] GLN-21; LEU-121 AND TRP-332.
RX PubMed=17344846; DOI=10.1038/nature05610;
RA Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G.,
RA Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S.,
RA Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.,
RA Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K.,
RA Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D.,
RA Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R.,
RA Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A.,
RA Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F.,
RA Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F.,
RA Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G.,
RA Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R.,
RA Futreal P.A., Stratton M.R.;
RT "Patterns of somatic mutation in human cancer genomes.";
RL Nature 446:153-158(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9Y6S9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9Y6S9-2; Sequence=VSP_036440, VSP_036441;
CC Name=4;
CC IsoId=Q9Y6S9-4; Sequence=VSP_017931, VSP_017932;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. S6 kinase subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU00159}.
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DR EMBL; AK054800; BAB70808.1; -; mRNA.
DR EMBL; AC006530; AAD30182.1; -; Genomic_DNA.
DR EMBL; BC004540; AAH04540.1; -; mRNA.
DR EMBL; AL390152; CAH10661.1; -; mRNA.
DR CCDS; CCDS9834.2; -. [Q9Y6S9-1]
DR RefSeq; NP_113652.2; NM_031464.4. [Q9Y6S9-1]
DR AlphaFoldDB; Q9Y6S9; -.
DR SMR; Q9Y6S9; -.
DR BioGRID; 123728; 11.
DR IntAct; Q9Y6S9; 44.
DR STRING; 9606.ENSP00000346644; -.
DR GlyGen; Q9Y6S9; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9Y6S9; -.
DR PhosphoSitePlus; Q9Y6S9; -.
DR BioMuta; RPS6KL1; -.
DR DMDM; 74762793; -.
DR EPD; Q9Y6S9; -.
DR MassIVE; Q9Y6S9; -.
DR MaxQB; Q9Y6S9; -.
DR PaxDb; Q9Y6S9; -.
DR PeptideAtlas; Q9Y6S9; -.
DR PRIDE; Q9Y6S9; -.
DR Antibodypedia; 25730; 192 antibodies from 29 providers.
DR DNASU; 83694; -.
DR Ensembl; ENST00000354625.6; ENSP00000346644.3; ENSG00000198208.12. [Q9Y6S9-1]
DR Ensembl; ENST00000555009.5; ENSP00000450660.1; ENSG00000198208.12. [Q9Y6S9-2]
DR Ensembl; ENST00000555647.5; ENSP00000452027.1; ENSG00000198208.12. [Q9Y6S9-1]
DR Ensembl; ENST00000557413.6; ENSP00000450567.1; ENSG00000198208.12. [Q9Y6S9-1]
DR GeneID; 83694; -.
DR KEGG; hsa:83694; -.
DR MANE-Select; ENST00000557413.6; ENSP00000450567.1; NM_031464.5; NP_113652.2.
DR UCSC; uc010tux.3; human. [Q9Y6S9-1]
DR CTD; 83694; -.
DR DisGeNET; 83694; -.
DR GeneCards; RPS6KL1; -.
DR HGNC; HGNC:20222; RPS6KL1.
DR HPA; ENSG00000198208; Tissue enhanced (brain).
DR neXtProt; NX_Q9Y6S9; -.
DR OpenTargets; ENSG00000198208; -.
DR PharmGKB; PA134868364; -.
DR VEuPathDB; HostDB:ENSG00000198208; -.
DR eggNOG; KOG0603; Eukaryota.
DR GeneTree; ENSGT00940000159815; -.
DR HOGENOM; CLU_014272_2_0_1; -.
DR InParanoid; Q9Y6S9; -.
DR OMA; SLPRCHV; -.
DR OrthoDB; 255297at2759; -.
DR PhylomeDB; Q9Y6S9; -.
DR TreeFam; TF323964; -.
DR PathwayCommons; Q9Y6S9; -.
DR SignaLink; Q9Y6S9; -.
DR BioGRID-ORCS; 83694; 12 hits in 1109 CRISPR screens.
DR GenomeRNAi; 83694; -.
DR Pharos; Q9Y6S9; Tdark.
DR PRO; PR:Q9Y6S9; -.
DR Proteomes; UP000005640; Chromosome 14.
DR RNAct; Q9Y6S9; protein.
DR Bgee; ENSG00000198208; Expressed in right hemisphere of cerebellum and 113 other tissues.
DR ExpressionAtlas; Q9Y6S9; baseline and differential.
DR Genevisible; Q9Y6S9; HS.
DR GO; GO:0005840; C:ribosome; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR CDD; cd05576; STKc_RPK118_like; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR007330; MIT_dom.
DR InterPro; IPR036181; MIT_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR035050; RPS6KL1.
DR InterPro; IPR035053; STK_RPK118-like.
DR PANTHER; PTHR15508:SF4; PTHR15508:SF4; 1.
DR Pfam; PF04212; MIT; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00745; MIT; 1.
DR SUPFAM; SSF116846; SSF116846; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Kinase; Nucleotide-binding;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..549
FT /note="Ribosomal protein S6 kinase-like 1"
FT /id="PRO_0000232641"
FT DOMAIN 87..115
FT /note="MIT"
FT DOMAIN 145..539
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 260..325
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..349
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 412
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 151..159
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 177
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT VAR_SEQ 131..161
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036440"
FT VAR_SEQ 223..255
FT /note="GTLWSHLLSQAHSRHSGLSSGSTQERMKAQLNP -> EQAWGPLSAPQAFGV
FT CRQPLKCGALAGRGGSRL (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_017931"
FT VAR_SEQ 256..549
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_017932"
FT VAR_SEQ 549
FT /note="G -> GTSLETHLRTLIHWLSGPKPDCPG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_036441"
FT VARIANT 21
FT /note="R -> Q (in dbSNP:rs7156590)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_041065"
FT VARIANT 121
FT /note="P -> L (in dbSNP:rs2286913)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_041066"
FT VARIANT 332
FT /note="R -> W (in dbSNP:rs55871101)"
FT /evidence="ECO:0000269|PubMed:17344846"
FT /id="VAR_041067"
FT CONFLICT 64
FT /note="D -> G (in Ref. 1; BAB70808)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 549 AA; 60036 MW; C5955B004EAC959A CRC64;
MSLVACECLP SPGLEPEPCS RARSQAHVYL EQIRNRVALG VPDMTKRDYL VDAATQIRLA
LERDVSEDYE AAFNHYQNGV DVLLRGIHVD PNKERREAVK LKITKYLRRA EEIFNCHLQR
PLSSGASPSA GFSSLRLRPI RTLSSAVEQL RGCRVVGVIE KVQLVQDPAT GGTFVVKSLP
RCHMVSRERL TIIPHGVPYM TKLLRYFVSE DSIFLHLEHV QGGTLWSHLL SQAHSRHSGL
SSGSTQERMK AQLNPHLNLL TPARLPSGHA PGQDRIALEP PRTSPNLLLA GEAPSTRPQR
EAEGEPTART STSGSSDLPK APGGHLHLQA RRAGQNSDAG PPRGLTWVPE GAGPVLGGCG
RGMDQSCLSA DGAGRGCGRA TWSVREEQVK QWAAEMLVAL EALHEQGVLC RDLHPGNLLL
DQAGHIRLTY FGQWSEVEPQ CCGEAVDNLY SAPEVGGISE LTEACDWWSF GSLLYELLTG
MALSQSHPSG IQAHTQLQLP EWLSRPAASL LTELLQFEPT RRLGMGEGGV SKLKSHPFFS
TIQWSKLVG
