ID   RPN1_ENCCU              Reviewed;         795 AA.
AC   Q8SS65;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=26S proteasome regulatory subunit RPN1;
GN   Name=RPN1; OrderedLocusNames=ECU04_0310;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16691553; DOI=10.1002/pmic.200500796;
RA   Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT   "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT   (microsporidia): a reference map for proteins expressed in late sporogonial
RT   stages.";
RL   Proteomics 6:3625-3635(2006).
CC   -!- FUNCTION: Acts as a regulatory subunit of the 26S proteasome which
CC       degrades poly-ubiquitinated proteins in the cytoplasm and in the
CC       nucleus. It is essential for the regulated turnover of proteins and for
CC       the removal of misfolded proteins. The proteasome is a multicatalytic
CC       proteinase complex that is characterized by its ability to cleave
CC       peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group
CC       at neutral or slightly basic pH (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: The 26S proteasome consists of a 20S proteasome core and two
CC       19S regulatory subunits. The 20S proteasome core is composed of 28
CC       subunits that are arranged in four stacked rings, resulting in a
CC       barrel-shaped structure. The two end rings are each formed by seven
CC       alpha subunits, and the two central rings are each formed by seven beta
CC       subunits. The catalytic chamber with the active sites is on the inside
CC       of the barrel (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in late sporogonial stages.
CC       {ECO:0000269|PubMed:16691553}.
CC   -!- SIMILARITY: Belongs to the proteasome subunit S2 family. {ECO:0000305}.
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DR   EMBL; AL590444; CAD25218.1; -; Genomic_DNA.
DR   RefSeq; NP_584714.1; NM_001041064.1.
DR   AlphaFoldDB; Q8SS65; -.
DR   STRING; 284813.Q8SS65; -.
DR   GeneID; 858862; -.
DR   KEGG; ecu:ECU04_0310; -.
DR   VEuPathDB; MicrosporidiaDB:ECU04_0310; -.
DR   HOGENOM; CLU_019273_0_0_1; -.
DR   InParanoid; Q8SS65; -.
DR   OMA; MAPPENP; -.
DR   OrthoDB; 229956at2759; -.
DR   Proteomes; UP000000819; Chromosome IV.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
DR   GO; GO:0030234; F:enzyme regulator activity; IEA:InterPro.
DR   GO; GO:0042176; P:regulation of protein catabolic process; IEA:InterPro.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR016643; 26S_Psome_Rpn1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR041433; RPN1_C.
DR   InterPro; IPR040892; RPN1_N.
DR   PANTHER; PTHR10943:SF1; PTHR10943:SF1; 1.
DR   Pfam; PF18051; RPN1_C; 1.
DR   Pfam; PF17781; RPN1_RPN2_N; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Proteasome; Reference proteome; Repeat.
FT   CHAIN           1..795
FT                   /note="26S proteasome regulatory subunit RPN1"
FT                   /id="PRO_0000382763"
FT   REPEAT          374..410
FT                   /note="PC 1"
FT   REPEAT          411..445
FT                   /note="PC 2"
FT   REPEAT          447..480
FT                   /note="PC 3"
FT   REPEAT          624..658
FT                   /note="PC 4"
SQ   SEQUENCE   795 AA;  89127 MW;  6654367B915F8516 CRC64;
     MEEGELKIIV ERIQDPDIDI QNNALNMLFD VTKSSHSKSI DTINFQYLAD NLDVLEDVCK
     RLEGNKKRWL CDIISAICVV DDERKLLAYR VEGNIIDLKE WGHLYVKKLI GCIADVKNNK
     MDFPFAKTRD VGRECIDFLF KHNAEFEAID FLVEVGGIET VLDYVDTHNY NRIVLYLEDM
     NSFVDLEEVI LKIYLKMGDH SRYVVGLIRR QKSKEAIEYV RSIEDRDYKK QCLYILARCN
     LYYEASDPEE KYILSNGYIK DVYREIGAEL EIDKPSKMDA ILKGFKYDKD TRQLASIGIA
     NGFVHMGYGR DPIFLPQEGD SRVPLDYEAI LGCDVPDLIS VFGSIGVIES WNSEKVMETL
     QEHIFADFSY RKTGSLLGLA LSGLRNFEER PAILALLSNN LQSSSSIHVI ATLLGIEAMF
     SGTQAEEVRE LLQPLMFSDS SEVVFFTSFT LGSVFCGSAD EDLTSLMLQT FVEKGKESET
     QFFRFLMLGL ASLFYRRKDV ECGIMEIGGA LSKHESILIK GFQYVGTGDS NVIESILTDS
     FTGDTDALLE SLGLLSCALV SMGDETSSQM VGRIVSSSLL LDSSHLRSVL PLCYSILYPS
     NPQVNVLDML EKSLNIGETN CIISTIVSLG LIGAGTLNSR ITKILDQQYS YYYKDSKVLP
     VLKIAQGLVS LGKGLLSISP LYFDKTTFMP KNTIGLFSTV FMLLDSSISP LVSSHAYMFF
     LLCQACTQKY VTCSEKINIR VGHPINTVGM VGEPKKLSSV QTHTSPVVLS EKIRAETDEN
     VCSSYIEDVL ILKKN