ID   RPN8_NEUCR              Reviewed;         352 AA.
AC   Q8WZY4; Q1K4Y6;
DT   31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=26S proteasome regulatory subunit rpn-8;
GN   Name=rpn-8; ORFNames=B24G3.180, NCU01547;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Acts as a regulatory subunit of the 26S proteasome which is
CC       involved in the ATP-dependent degradation of ubiquitinated proteins.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase M67A family. {ECO:0000305}.
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DR   EMBL; AL670002; CAD21233.1; -; Genomic_DNA.
DR   EMBL; CM002237; EAA27014.1; -; Genomic_DNA.
DR   RefSeq; XP_956250.1; XM_951157.3.
DR   AlphaFoldDB; Q8WZY4; -.
DR   SMR; Q8WZY4; -.
DR   STRING; 5141.EFNCRP00000001798; -.
DR   MEROPS; M67.973; -.
DR   PRIDE; Q8WZY4; -.
DR   EnsemblFungi; EAA27014; EAA27014; NCU01547.
DR   GeneID; 3872410; -.
DR   KEGG; ncr:NCU01547; -.
DR   VEuPathDB; FungiDB:NCU01547; -.
DR   HOGENOM; CLU_027018_3_0_1; -.
DR   InParanoid; Q8WZY4; -.
DR   Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR   GO; GO:1990023; C:mitotic spindle midzone; IEA:EnsemblFungi.
DR   GO; GO:0000502; C:proteasome complex; IBA:GO_Central.
DR   GO; GO:0008541; C:proteasome regulatory particle, lid subcomplex; IEA:EnsemblFungi.
DR   GO; GO:0034515; C:proteasome storage granule; IEA:EnsemblFungi.
DR   GO; GO:0070122; F:isopeptidase activity; IEA:InterPro.
DR   GO; GO:0140492; F:metal-dependent deubiquitinase activity; IEA:InterPro.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   CDD; cd08062; MPN_RPN7_8; 1.
DR   InterPro; IPR000555; JAMM/MPN+_dom.
DR   InterPro; IPR037518; MPN.
DR   InterPro; IPR033858; MPN_RPN7_8.
DR   InterPro; IPR024969; Rpn11/EIF3F_C.
DR   Pfam; PF01398; JAB; 1.
DR   Pfam; PF13012; MitMem_reg; 1.
DR   SMART; SM00232; JAB_MPN; 1.
DR   PROSITE; PS50249; MPN; 1.
PE   3: Inferred from homology;
KW   Proteasome; Reference proteome.
FT   CHAIN           1..352
FT                   /note="26S proteasome regulatory subunit rpn-8"
FT                   /id="PRO_0000213949"
FT   DOMAIN          16..152
FT                   /note="MPN"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01182"
FT   REGION          303..352
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   352 AA;  39104 MW;  DA977213816266AE CRC64;
     MPATTAETLS LVNRSVSVAP LVLLSVVDHY NRTQANKSKS KRVVGVLLGQ NDGKNVRVSN
     SFAVPFEEDD KDPSVWFLDH NYVESMNDMF KKVNAREKLI GWYHSGPKLR ASDLDINELF
     KRYTPNPLLV IVDVQPKETG VPTDAYFAVD EIKDDGTTTS KTFVHTPSII EAEEAEEIGV
     EHLLRDIRDV AVGTLSTRIT NQMRSLQGLH HRLRDIQAYL QKVLDGQLPV NHAILGNLQD
     VFNLLPNLST PKSGPGATGT NADSELNHAM SIKTNDQLMA IYLSSLIRAI TAFHDLIENK
     IQNRQQQEEN DAKKKEGENG EKKEGADKKE GSPAAANGES KEKENSPKEK KK