ID   RPNB_ECOLI              Reviewed;         296 AA.
AC   P77768;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Recombination-promoting nuclease RpnB {ECO:0000303|PubMed:28096446};
DE            EC=3.1.21.-;
GN   Name=rpnB {ECO:0000303|PubMed:28096446}; Synonyms=yfcI;
GN   OrderedLocusNames=b2305, JW2302;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12;
RX   PubMed=28096446; DOI=10.1128/jb.00787-16;
RA   Kingston A.W., Ponkratz C., Raleigh E.A.;
RT   "The Rpn (YhgA-like) proteins of Escherichia coli K-12 and their
RT   contribution to RecA-independent horizontal transfer.";
RL   J. Bacteriol. 0:0-0(2017).
CC   -!- FUNCTION: A low activity DNA endonuclease yielding 3'-hydroxyl ends
CC       (Probable). Upon expression enhances RecA-independent DNA recombination
CC       19-fold, concomitantly reducing viability by 98% and inducing DNA
CC       damage as measured by induction of the SOS repair response.
CC       {ECO:0000269|PubMed:28096446, ECO:0000305|PubMed:28096446}.
CC   -!- DISRUPTION PHENOTYPE: A quadruple rpnA-rpnB-rpnC-rpnD deletion shows no
CC       change in basal RecA-independent recombination.
CC       {ECO:0000269|PubMed:28096446}.
CC   -!- SIMILARITY: Belongs to the Rpn/YhgA-like nuclease family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00096; AAC75365.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16142.1; -; Genomic_DNA.
DR   PIR; G65002; G65002.
DR   RefSeq; NP_416808.1; NC_000913.3.
DR   RefSeq; WP_000156149.1; NZ_LN832404.1.
DR   AlphaFoldDB; P77768; -.
DR   SMR; P77768; -.
DR   IntAct; P77768; 3.
DR   STRING; 511145.b2305; -.
DR   PaxDb; P77768; -.
DR   PRIDE; P77768; -.
DR   EnsemblBacteria; AAC75365; AAC75365; b2305.
DR   EnsemblBacteria; BAA16142; BAA16142; BAA16142.
DR   GeneID; 946787; -.
DR   KEGG; ecj:JW2302; -.
DR   KEGG; eco:b2305; -.
DR   PATRIC; fig|511145.12.peg.2400; -.
DR   EchoBASE; EB3865; -.
DR   eggNOG; COG5464; Bacteria.
DR   HOGENOM; CLU_059548_1_0_6; -.
DR   InParanoid; P77768; -.
DR   OMA; ERIDKSY; -.
DR   PhylomeDB; P77768; -.
DR   BioCyc; EcoCyc:G7197-MON; -.
DR   PRO; PR:P77768; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:1990238; F:double-stranded DNA endodeoxyribonuclease activity; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IDA:UniProtKB.
DR   InterPro; IPR010106; RpnA.
DR   InterPro; IPR006842; Transposase_31.
DR   Pfam; PF04754; Transposase_31; 1.
DR   TIGRFAMs; TIGR01784; T_den_put_tspse; 1.
PE   3: Inferred from homology;
KW   DNA recombination; Endonuclease; Hydrolase; Magnesium; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..296
FT                   /note="Recombination-promoting nuclease RpnB"
FT                   /id="PRO_0000169193"
SQ   SEQUENCE   296 AA;  34179 MW;  4FC384A2958F0D5D CRC64;
     MTISTTSTPH DAVFKSFLRH PDTARDFIDI HLPAPLRKLC DLTTLKLEPN SFIDEDLRQY
     YSDLLWSVKT QEGVGYIYVV IEHQSKPEEL MAFRMMRYSI AAMQNHLDAG YKELPLVLPM
     LFYHGCRSPY PYSLCWLDEF AEPAIARKIY SSAFPLVDIT VVPDDEIMQH RKMALLELIQ
     KHIRQRDLLG LVDQIVSLLV TGNTNDRQLK ALFNYVLQTG DAQRFRAFIG EIAERAPQEK
     EKLMTIADRL REEGAMQGKH EEALRIAQEM LDRGLDRELV MMVTRLSPDD LIAQSH