ID   RPNE_ECOLI              Reviewed;         299 AA.
AC   P37014; P77771;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 3.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Inactive recombination-promoting nuclease-like protein RpnE;
GN   Name=yfaD; OrderedLocusNames=b2244, JW2238;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-86.
RC   STRAIN=K12;
RX   PubMed=3286606; DOI=10.1128/jb.170.6.2448-2456.1988;
RA   Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F.,
RA   Weiner J.H.;
RT   "Nucleotide sequence and gene-polypeptide relationships of the glpABC
RT   operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of
RT   Escherichia coli K-12.";
RL   J. Bacteriol. 170:2448-2456(1988).
RN   [5]
RP   IDENTIFICATION.
RX   PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA   Borodovsky M., Rudd K.E., Koonin E.V.;
RT   "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT   genome.";
RL   Nucleic Acids Res. 22:4756-4767(1994).
RN   [6]
RP   LACK OF FUNCTION.
RC   STRAIN=K12;
RX   PubMed=28096446; DOI=10.1128/jb.00787-16;
RA   Kingston A.W., Ponkratz C., Raleigh E.A.;
RT   "The Rpn (YhgA-like) proteins of Escherichia coli K-12 and their
RT   contribution to RecA-independent horizontal transfer.";
RL   J. Bacteriol. 0:0-0(2017).
CC   -!- FUNCTION: Upon expression has no effect on RecA-independent DNA
CC       recombination, cell viability or DNA damage (PubMed:28096446).
CC       {ECO:0000269|PubMed:28096446}.
CC   -!- SIMILARITY: Belongs to the Rpn/YhgA-like nuclease family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00096; AAC75304.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16063.1; -; Genomic_DNA.
DR   EMBL; M20938; AAA83867.1; -; Genomic_DNA.
DR   PIR; B64995; B64995.
DR   RefSeq; NP_416747.1; NC_000913.3.
DR   RefSeq; WP_000140592.1; NZ_LN832404.1.
DR   AlphaFoldDB; P37014; -.
DR   SMR; P37014; -.
DR   BioGRID; 4262943; 9.
DR   IntAct; P37014; 3.
DR   STRING; 511145.b2244; -.
DR   PaxDb; P37014; -.
DR   PRIDE; P37014; -.
DR   EnsemblBacteria; AAC75304; AAC75304; b2244.
DR   EnsemblBacteria; BAA16063; BAA16063; BAA16063.
DR   GeneID; 946737; -.
DR   KEGG; ecj:JW2238; -.
DR   KEGG; eco:b2244; -.
DR   PATRIC; fig|511145.12.peg.2334; -.
DR   EchoBASE; EB2228; -.
DR   eggNOG; COG5464; Bacteria.
DR   HOGENOM; CLU_059548_1_0_6; -.
DR   InParanoid; P37014; -.
DR   OMA; NGIGERW; -.
DR   PhylomeDB; P37014; -.
DR   BioCyc; EcoCyc:EG12323-MON; -.
DR   PRO; PR:P37014; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:1990238; F:double-stranded DNA endodeoxyribonuclease activity; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IBA:GO_Central.
DR   InterPro; IPR010106; RpnA.
DR   InterPro; IPR006842; Transposase_31.
DR   Pfam; PF04754; Transposase_31; 1.
DR   TIGRFAMs; TIGR01784; T_den_put_tspse; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..299
FT                   /note="Inactive recombination-promoting nuclease-like
FT                   protein RpnE"
FT                   /id="PRO_0000169172"
FT   CONFLICT        86
FT                   /note="S -> C (in Ref. 4; AAA83867)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   299 AA;  34923 MW;  593E99411667BF1B CRC64;
     MTESTTSSPH DAVFKTFMFT PETARDFLEI HLPEPLRKLC NLQTLRLEPT SFIEKSLRAY
     YSDVLWSVET SDGDGYIYCV IEHQSSAEKN MAFRLMRYAT AAMQRHQDKG YDRVPLVVPL
     LFYHGETSPY PYSLNWLDEF DDPQLARQLY TEAFLLVDIT IVPDDEIMQH RRIALLELIQ
     KHIRDRDLIG MVDRITTLLV RGFTNDSQLQ TLFNYLLQCG DTSRFTRFIE EIAERSPLQK
     ERLMTIAERL RQEGHQIGWQ EGMHEQAIKI ALRMLEQGFE REIVLATTQL TDADIPNCH