ID   RPO1A_TOBAC             Reviewed;         155 AA.
AC   Q8L6J4;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 2.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=DNA-directed RNA polymerase 1A;
DE            EC=2.7.7.6;
DE   AltName: Full=NictaRpoT1-syl;
DE   AltName: Full=T7 bacteriophage-type single subunit RNA polymerase 1A;
DE   Flags: Fragment;
GN   Name=RPOT1-SYL;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12061895; DOI=10.1046/j.1365-313x.2002.01318.x;
RA   Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T.;
RT   "Six active phage-type RNA polymerase genes in Nicotiana tabacum.";
RL   Plant J. 30:625-637(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC95023.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AJ416572; CAC95023.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; Q8L6J4; -.
DR   SMR; Q8L6J4; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0034245; C:mitochondrial DNA-directed RNA polymerase complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0006390; P:mitochondrial transcription; IBA:GO_Central.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           <1..155
FT                   /note="DNA-directed RNA polymerase 1A"
FT                   /id="PRO_0000087751"
FT   ACT_SITE        88
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   155 AA;  17959 MW;  854FC073023BE8BA CRC64;
     IIAMENHPVR WTTPLGLPVV QPYRKLGRHL IKTSLQILTL QRETDKVMVK RQRTAFPPNF
     VHSLDGSHMM MTAIACKESG LSFAGVHDSY WTHASDVDQM NKILREKFVE LYDAPILENL
     LESFQQSFPD LQFPPLPERG DFDLREVLES PYFFN