ID   RPO1B_TOBAC             Reviewed;        1002 AA.
AC   Q8L6J5; Q8L6K2;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=DNA-directed RNA polymerase 1B, mitochondrial;
DE            EC=2.7.7.6;
DE   AltName: Full=NictaRpoT1-tom;
DE   AltName: Full=T7 bacteriophage-type single subunit RNA polymerase 1B;
DE   Flags: Precursor;
GN   Name=RPOT1-TOM;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND SUBCELLULAR LOCATION.
RX   PubMed=12061895; DOI=10.1046/j.1365-313x.2002.01318.x;
RA   Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T.;
RT   "Six active phage-type RNA polymerase genes in Nicotiana tabacum.";
RL   Plant J. 30:625-637(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:12061895}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; AJ302018; CAC82574.2; -; mRNA.
DR   EMBL; AJ416571; CAC95022.1; -; Genomic_DNA.
DR   RefSeq; NP_001312318.1; NM_001325389.1.
DR   AlphaFoldDB; Q8L6J5; -.
DR   SMR; Q8L6J5; -.
DR   GeneID; 107784816; -.
DR   KEGG; nta:107784816; -.
DR   OMA; AFTENHV; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0034245; C:mitochondrial DNA-directed RNA polymerase complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0006390; P:mitochondrial transcription; IBA:GO_Central.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   Gene3D; 1.10.287.260; -; 1.
DR   InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   InterPro; IPR029262; RPOL_N.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   Pfam; PF14700; RPOL_N; 1.
DR   SMART; SM01311; RPOL_N; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   2: Evidence at transcript level;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Reference proteome; Transcription; Transferase; Transit peptide.
FT   TRANSIT         1..21
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..1002
FT                   /note="DNA-directed RNA polymerase 1B, mitochondrial"
FT                   /id="PRO_0000046033"
FT   ACT_SITE        703
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        778
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        935
FT                   /evidence="ECO:0000250"
FT   CONFLICT        502
FT                   /note="D -> N (in Ref. 1; CAC82574)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1002 AA;  113532 MW;  06451D1652707D72 CRC64;
     MWRYISKHAY SRKFRNSHDS ALLGFSQYSS SFGKTRPLQC LCEESTTHPN LGLSQNSIFS
     RISRKVRHLE GICEESSKNP HLGLSQNSTF SSVKGDFRIC GKRGSGSLGR LRSYGSAAEA
     IVSTSEEDID EIQELIEEMD KENEALKANL QPKQPKTIGG MGVGKYNFLR RRQIKVETEA
     WEEAAKEYQE LLMDMCEQKL APNLPYMKSL FLGWFEPLRD AIAAEQKLCD EGKNRGAYAP
     FFDQLPAEMM AVITMHKLMG LLMTGGGTGS ARVVQAASYI GEAIEHEARI HRFLEKTKKS
     NALSGDLEET PGDMMKERER LRKKVKILMK KQKLRQVRKI VKQQDDEKPW GQDNLVKVGC
     RLIQILMETA YIQPPNDQLD DGPPDIRPAF VHTLKTVETM KGSRRYGVIQ CDPLVRKGLD
     KTARHMVIPY MPMLVPPQSW LGYDKGGYLF LPSYIMRTHG AKQQREAVKR VPKKQLEPVF
     QALDTLGNTK WRVNRKVLGI VDRIWASGGR LADLVDREDV PLPEAPDTED EAEIRKWKWK
     VKGVKKENCE RHSQRCDIEL KLAVARKMKD EDGFYYPHNL DFRGRAYPMH PYLNHLGSDL
     CRGILEFAEG RPLGTSGLRW LKIHLANVYG GGVDKLSYEG RVAFSENHLE DIFDSAERPL
     EGKRWWLGAE DPFQCLATCI NIAEALRSPS PETAISYMPI HQDGSCNGLQ HYAALGRDKL
     GAAAVNLVAG DKPADVYSGI AARVLDIMKR DAAKDPANDP NVMRARLLIN QVDRKLVKQT
     VMTSVYGVTY IGARDQIKKR LKERGVIEDD NELFAAACYA AKTTLTALGE MFEAARSIMS
     WLGDCAKIIA MENHPVRWTT PLGLPVVQPY RKLGRHLIKT SLQILTLQRE TDKVMVKRQR
     TAFPPNFVHS LDGSHMMMTA IACKESGLSF AGVHDSYWTH ACDVDQMNKI LREKFVELYD
     APILENLLES FQQSFPDLQF PPLPERGDFD LREVLESPYF FN