RPO1C_METJA
ID RPO1C_METJA Reviewed; 859 AA.
AC Q58446;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=DNA-directed RNA polymerase subunit Rpo1C {ECO:0000255|HAMAP-Rule:MF_00411};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00411};
DE AltName: Full=DNA-directed RNA polymerase subunit A'' {ECO:0000255|HAMAP-Rule:MF_00411};
DE Contains:
DE RecName: Full=Mja rpo1C intein;
DE AltName: Full=Mja rpoA'' intein;
DE AltName: Full=Mja rpoA2 intein;
GN Name=rpo1C {ECO:0000255|HAMAP-Rule:MF_00411};
GN Synonyms=rpoA2 {ECO:0000255|HAMAP-Rule:MF_00411}; OrderedLocusNames=MJ1043;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC transcription of DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. Forms part of the jaw domain.
CC {ECO:0000255|HAMAP-Rule:MF_00411}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00411};
CC -!- SUBUNIT: Part of the RNA polymerase complex. {ECO:0000255|HAMAP-
CC Rule:MF_00411}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00411}.
CC -!- PTM: This protein undergoes a protein self splicing that involves a
CC post-translational excision of the intervening region (intein) followed
CC by peptide ligation. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000255|HAMAP-Rule:MF_00411}.
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DR EMBL; L77117; AAB99047.1; -; Genomic_DNA.
DR PIR; B64430; B64430.
DR RefSeq; WP_010870556.1; NC_000909.1.
DR AlphaFoldDB; Q58446; -.
DR SMR; Q58446; -.
DR STRING; 243232.MJ_1043; -.
DR EnsemblBacteria; AAB99047; AAB99047; MJ_1043.
DR GeneID; 1451940; -.
DR KEGG; mja:MJ_1043; -.
DR eggNOG; arCOG03145; Archaea.
DR eggNOG; arCOG04256; Archaea.
DR HOGENOM; CLU_351851_0_0_2; -.
DR InParanoid; Q58446; -.
DR OMA; KVHWKRI; -.
DR OrthoDB; 66811at2157; -.
DR PhylomeDB; Q58446; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR CDD; cd06528; RNAP_A; 1.
DR Gene3D; 3.10.28.10; -; 1.
DR HAMAP; MF_00411; RNApol_arch_Rpo1C; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR012757; RPO1C.
DR PANTHER; PTHR19376; PTHR19376; 2.
DR Pfam; PF14528; LAGLIDADG_3; 2.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; SSF51294; 1.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR01443; intein_Cterm; 1.
DR TIGRFAMs; TIGR01445; intein_Nterm; 1.
DR TIGRFAMs; TIGR02389; RNA_pol_rpoA2; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW Autocatalytic cleavage; Cytoplasm; DNA-binding;
KW DNA-directed RNA polymerase; Endonuclease; Hydrolase; Intron homing;
KW Nuclease; Nucleotidyltransferase; Protein splicing; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..859
FT /note="DNA-directed RNA polymerase subunit Rpo1C"
FT /id="PRO_0000453741"
FT CHAIN 1..75
FT /note="DNA-directed RNA polymerase subunit Rpo1C, 1st part"
FT /id="PRO_0000031065"
FT CHAIN 76..546
FT /note="Mja rpo1C intein"
FT /id="PRO_0000031066"
FT CHAIN 547..859
FT /note="DNA-directed RNA polymerase subunit Rpo1C, 2nd part"
FT /id="PRO_0000031067"
SQ SEQUENCE 859 AA; 97087 MW; A05799E007899015 CRC64;
MDMEALKQKI EGLDIPQSLK DELFEKLSKE KDLTEEMVDE IIDEVVNAYR KALVEPYEAV
GIVAAQSIGE PGTQMSLPYE EKIIIKEGEF IKPVEIGKLV DEMIERFGFE KIGNSEVCDL
PIDIYALSLD QDEKVHWKRI ISCIRHKHNG KLIKIKTKSG REITATPYHS FVIRKDNKII
PVKGSELKIG DRIPVVKHIP ANCVEAINIS DYVSGNYVVD NINNKIAPKI NGKSIPNNIK
LDYDFGYFIG IYLAEGSVTK YFVSISNVDE LILNKIRAFA DKLGLNYGEY DNNNGFAESH
DIRIYSSTLA EFLSNFGTSS NTKKIAEFVF GANKEFVRGL IRGYFDGDGN VNADRKVIRV
TSNSKELIDG IAILLARFNI FSIKTKTKNQ FVLIIPHRYA KKFHEEINFS VEKKKSELER
LVSSLNDDKT YDSIDMIPSI GDALTKLGEK VDYPKVILKK FERKQKIGRA TLQRHLRRIE
ELAVKKGVNI LALKEYWLLK KAVESDVIWD EIVKIEEISC DKKYVYDISV EGLETFTTFD
GVLTHNTMRT FHYAGVAEIN VTLGLPRMIE IVDARKEPST PIMTIYLKEE YKDNREKAEE
IAKEIESLTL GSIAESISID LWTQSIKVEL DENRLADRGL TIDDVIEAIK KKLKVKIDVD
GTTLYLKIKT PSIKALRKRI PKIKNIQLKG IPGIERVLVK KEGGEYVLYT QGSNLREVFK
IDGVDTTRTI TNNIIEIQEV LGIEAARNAI INEMRNTLEQ QGLEVDIRHL MLVADIMTAD
GEVKPIGRHG VAGEKGSVLA RAAFEETVKH LYAAAERGDV DKLKGVIENV IVGKPIYLGT
GCVELTIDRE YEEGKNMEE
