RPO1N_SULAC
ID RPO1N_SULAC Reviewed; 880 AA.
AC P11512; Q4JAV7;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=DNA-directed RNA polymerase subunit Rpo1N {ECO:0000255|HAMAP-Rule:MF_00863};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00863, ECO:0000269|Ref.4};
DE AltName: Full=DNA-directed RNA polymerase subunit A {ECO:0000303|PubMed:2501756};
DE AltName: Full=DNA-directed RNA polymerase subunit A' {ECO:0000255|HAMAP-Rule:MF_00863, ECO:0000303|Ref.4};
GN Name=rpo1N {ECO:0000255|HAMAP-Rule:MF_00863};
GN Synonyms=rpoA {ECO:0000303|PubMed:2501756},
GN rpoA1 {ECO:0000255|HAMAP-Rule:MF_00863, ECO:0000303|PubMed:1729711};
GN OrderedLocusNames=Saci_0692;
OS Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS 15157 / NCIMB 11770).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfolobus.
OX NCBI_TaxID=330779;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=2501756; DOI=10.1093/nar/17.12.4517;
RA Puehler G., Lottspeich F., Zillig W.;
RT "Organization and nucleotide sequence of the genes encoding the large
RT subunits A, B and C of the DNA-dependent RNA polymerase of the
RT archaebacterium Sulfolobus acidocaldarius.";
RL Nucleic Acids Res. 17:4517-4534(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT "The genome of Sulfolobus acidocaldarius, a model organism of the
RT Crenarchaeota.";
RL J. Bacteriol. 187:4992-4999(2005).
RN [3]
RP SUBUNIT.
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=1729711; DOI=10.1073/pnas.89.1.407;
RA Klenk H.-P., Palm P., Lottspeich F., Zillig W.;
RT "Component H of the DNA-dependent RNA polymerases of Archaea is homologous
RT to a subunit shared by the three eucaryal nuclear RNA polymerases.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:407-410(1992).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, COFACTOR, AND SUBUNIT.
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX DOI=10.1016/S0723-2020(11)80337-1;
RA Lanzendorfer M., Langer D., Hain J., Klenk H.-P., Holz I., Arnold-Ammer I.,
RA Zillig W.;
RT "Structure and Function of the DNA-Dependent RNA Polymerase of
RT Sulfolobus.";
RL Syst. Appl. Microbiol. 16:656-664(1994).
CC -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC transcription of DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. Forms the clamp head domain.
CC {ECO:0000255|HAMAP-Rule:MF_00863, ECO:0000269|Ref.4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00863,
CC ECO:0000269|Ref.4};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00863};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00863, ECO:0000305|Ref.4};
CC Note=Binds at least 2 Zn(2+) per subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00863};
CC -!- SUBUNIT: Part of the 13-subunit RNA polymerase complex.
CC {ECO:0000269|PubMed:1729711, ECO:0000269|Ref.4}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00863}.
CC -!- MISCELLANEOUS: Corresponds to about the first two-thirds of the
CC bacterial beta' subunit. {ECO:0000269|PubMed:2501756}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000255|HAMAP-Rule:MF_00863, ECO:0000305|PubMed:2501756}.
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DR EMBL; X14818; CAA32925.1; -; Genomic_DNA.
DR EMBL; CP000077; AAY80072.1; -; Genomic_DNA.
DR PIR; B33926; B33926.
DR RefSeq; WP_011277574.1; NC_007181.1.
DR PDB; 7OK0; EM; 2.90 A; A=1-880.
DR PDB; 7OQ4; EM; 3.27 A; A=1-880.
DR PDB; 7OQY; EM; 2.61 A; A=1-880.
DR PDBsum; 7OK0; -.
DR PDBsum; 7OQ4; -.
DR PDBsum; 7OQY; -.
DR AlphaFoldDB; P11512; -.
DR SMR; P11512; -.
DR STRING; 330779.Saci_0692; -.
DR PRIDE; P11512; -.
DR EnsemblBacteria; AAY80072; AAY80072; Saci_0692.
DR GeneID; 3472538; -.
DR KEGG; sai:Saci_0692; -.
DR PATRIC; fig|330779.12.peg.660; -.
DR eggNOG; arCOG04257; Archaea.
DR HOGENOM; CLU_000487_3_1_2; -.
DR OMA; AVCPPYN; -.
DR Proteomes; UP000001018; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IDA:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd02582; RNAP_archeal_A; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 4.10.860.120; -; 2.
DR HAMAP; MF_00863; RNApol_arch_Rpo1N; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR InterPro; IPR012758; RPO1N.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR TIGRFAMs; TIGR02390; RNA_pol_rpoA1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-binding; DNA-directed RNA polymerase;
KW Magnesium; Metal-binding; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase; Zinc.
FT CHAIN 1..880
FT /note="DNA-directed RNA polymerase subunit Rpo1N"
FT /id="PRO_0000074007"
FT BINDING 58
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 61
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 68
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 71
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 98
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 101
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 146
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 149
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 456
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 458
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT BINDING 460
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00863"
FT CONFLICT 56
FT /note="Q -> E (in Ref. 1; CAA32925)"
FT /evidence="ECO:0000305"
FT STRAND 4..10
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 16..22
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 35..37
FT /evidence="ECO:0007829|PDB:7OQ4"
FT TURN 47..49
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 53..55
FT /evidence="ECO:0007829|PDB:7OK0"
FT STRAND 59..62
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 65..67
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 73..82
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 87..95
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 99..101
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:7OQ4"
FT HELIX 108..121
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 122..124
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 126..141
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 147..149
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 156..159
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 160..162
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 163..166
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 168..171
FT /evidence="ECO:0007829|PDB:7OK0"
FT HELIX 177..184
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 189..194
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 199..201
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 204..207
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 208..214
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 217..219
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 225..227
FT /evidence="ECO:0007829|PDB:7OK0"
FT HELIX 234..254
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 261..277
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 288..292
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 298..300
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 303..307
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 308..312
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 316..328
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 336..339
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 341..346
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 347..352
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 355..357
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 358..366
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 371..373
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 375..379
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 385..387
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 388..390
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 394..399
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 406..410
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 416..420
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 427..429
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 430..437
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 439..445
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 447..449
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 450..453
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 457..459
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 461..465
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 470..479
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 482..485
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 489..492
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 493..495
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 501..510
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 518..525
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 527..529
FT /evidence="ECO:0007829|PDB:7OQ4"
FT STRAND 538..543
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 545..547
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 548..552
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 553..555
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 562..565
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 567..570
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 576..578
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 581..583
FT /evidence="ECO:0007829|PDB:7OK0"
FT STRAND 585..591
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 593..595
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 600..603
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 611..619
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 621..642
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 648..651
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 655..680
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 692..694
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 695..717
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 721..723
FT /evidence="ECO:0007829|PDB:7OK0"
FT HELIX 724..730
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 737..744
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 764..766
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 776..779
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 786..788
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 792..801
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 803..805
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 814..827
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 830..832
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 838..840
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 845..850
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 851..853
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 859..863
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 868..876
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 877..879
FT /evidence="ECO:0007829|PDB:7OQY"
SQ SEQUENCE 880 AA; 99790 MW; 4CECAA0D5D16EA62 CRC64;
MSEKIIRGVK FGVLSPNEIR QMSVTAIITS EVYDEDGTPI EGGVMDPKLG VIEPGQKCPV
CGNTLAGCPG HFGHIELIKP VIHIGYVKHI YDFLRSTCWR CGRIKIKEQD LERYKRIYNA
IKLRWPSAAR RLVEYIKKIS IKNLECPHCG EKQFKIKLEK PYNFNEERNG SIVKLSPSEI
RDRLERIPDS DVELLGYDPK SSRPEWMILT VLPVPPITIR PSITIESGIR AEDDLTHKLV
DIIRLNERLK ESIEAGAPQL IIEDLWDLLQ YHVATYFDNE IPGLPPAKHR SGRPLRTLAQ
RLKGKEGRFR GNLSGKRVDF SARTVISPDP NLSIDEVGIP YTIARMLTVP ERVTNINIER
IRQYIINGPD KWPGANYVIK PDGRRIDLRY VKDRKELASS ITAGYVVERH LVDGDVVLFN
RQPSLHRISM MAHKVRVLPG RTFRLNLLDC PPYNADFDGD EMNLHVPQSE EAIAEARELM
LVHKNIITPR YGGPIIGGGQ DYISGAYLLS VKTTLLTVEE VATILGVTDF VGELGEPAIL
APKPYYTGKQ VISLFLPKDF NFHGPANISK GPRACKDEIC PHDSFIVIKN GLLLEGVFDK
KAIGNQQPES MLHWSIREYG TEYGKWLMDN VFKMFIRFLE MRGFTMTLED ITIPDEAQNE
ITTKIKEGYS QVDEYIRKFN EGQLEPIPGR TIEESLESYI LDTLDKLRKV AGEIATKYLD
PFNNVYIMAI TGARGSELNI TQMTALLGQQ SVRGERIRRG YRERTLSLFK YGDIAPEARG
FVKNSFMRGL SPYEMFFHAA GGREGLVDTA VKTSQSGYMQ RRLINALSDL RIEYDGTVRS
LYGDIVQVVY GDDAVHPMYS AHSKSVNVNR VIERVIGWKR
