ID   RPO2A_TOBAC             Reviewed;         332 AA.
AC   Q8L6J2;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=DNA-directed RNA polymerase 2A;
DE            EC=2.7.7.6;
DE   AltName: Full=NictaRpoT2-syl;
DE   AltName: Full=T7 bacteriophage-type single subunit RNA polymerase 2A;
DE   Flags: Fragment;
GN   Name=RPOT2-SYL;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=12061895; DOI=10.1046/j.1365-313x.2002.01318.x;
RA   Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T.;
RT   "Six active phage-type RNA polymerase genes in Nicotiana tabacum.";
RL   Plant J. 30:625-637(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; AJ416574; CAC95025.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8L6J2; -.
DR   SMR; Q8L6J2; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0034245; C:mitochondrial DNA-directed RNA polymerase complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0006390; P:mitochondrial transcription; IBA:GO_Central.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW   Transcription; Transferase.
FT   CHAIN           <1..332
FT                   /note="DNA-directed RNA polymerase 2A"
FT                   /id="PRO_0000087752"
FT   ACT_SITE        33
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        108
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        265
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   332 AA;  37084 MW;  70DEA538C9B478B7 CRC64;
     DPFQCLAVCI NLSEAVRSSS PETSVSHIPV HQDGSCNGLQ HYAALGRDKL GAAAVNLVAG
     EKPADVYSGI AARVLDIMKR DAQRDPAEFP DAVRARVLVN QVDRKLVKQT VMTSVYGVTY
     IGARDQIKRR LKERGAIADD SELFGAACYA AKVTLTALGE MFEAARSIMT WLGECAKIIA
     SENEPVRWTT PLGLPVVQPY RKIGRHLIKT SLQILTLQRE TEKVMVKRQR TAFPPNFIHS
     LDGSHMMMTA VACRRAGLNF AGVHDSYWTH ACDVDKLNRI LREKFVELYE APILEKLLES
     FQTSYPTLLF PPLPERGDFD MRDVLESPYF FN