ID   RPO2B_TOBAC             Reviewed;        1021 AA.
AC   Q8L6J3; Q8L6J7;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=DNA-directed RNA polymerase 2B, chloroplastic/mitochondrial;
DE            EC=2.7.7.6;
DE   AltName: Full=NictaRpoT2-tom;
DE   AltName: Full=T7 bacteriophage-type single subunit RNA polymerase 2B;
DE   Flags: Precursor;
GN   Name=RPOT2-TOM;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND SUBCELLULAR LOCATION.
RX   PubMed=12061895; DOI=10.1046/j.1365-313x.2002.01318.x;
RA   Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T.;
RT   "Six active phage-type RNA polymerase genes in Nicotiana tabacum.";
RL   Plant J. 30:625-637(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000269|PubMed:12061895}. Mitochondrion
CC       {ECO:0000269|PubMed:12061895}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
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DR   EMBL; AJ416569; CAC95020.1; -; mRNA.
DR   EMBL; AJ416573; CAC95024.1; -; Genomic_DNA.
DR   RefSeq; NP_001311818.1; NM_001324889.1.
DR   AlphaFoldDB; Q8L6J3; -.
DR   SMR; Q8L6J3; -.
DR   GeneID; 107765810; -.
DR   KEGG; nta:107765810; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0034245; C:mitochondrial DNA-directed RNA polymerase complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0006390; P:mitochondrial transcription; IBA:GO_Central.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   Gene3D; 1.10.287.260; -; 1.
DR   InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   InterPro; IPR029262; RPOL_N.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   Pfam; PF14700; RPOL_N; 1.
DR   SMART; SM01311; RPOL_N; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; DNA-directed RNA polymerase; Mitochondrion;
KW   Nucleotidyltransferase; Plastid; Reference proteome; Transcription;
KW   Transferase; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast and mitochondrion"
FT   CHAIN           ?..1021
FT                   /note="DNA-directed RNA polymerase 2B,
FT                   chloroplastic/mitochondrial"
FT                   /id="PRO_0000046034"
FT   REGION          315..337
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        722
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        797
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        954
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1021 AA;  116163 MW;  D4F0CC22CD0AE6C1 CRC64;
     MSSTKTPISL TIKLNQFTDK PTGLDINRYH NSPIMWRNII KQLSSRTPQK LLFSSKNRTY
     SFLGFGQDSV FKDNTKFRSL IPISCSNIVM GFQNLGEYLP GDEFLSRPLL KNQVNSNDFC
     CRKSYASVAE AVAVSSTDAE EDVSVVDEVQ ELLTELKKEE KKQFAFRRRK QRMLTSGMGH
     RKYQTLKRRQ VKVETEAWEQ AAKEYKELLF DMCEQKLAPN LPYVKSLFLG WFEPLRDKIA
     EEQELCSQGK SKAAYAKYLY QLPADMMAVI TMHKLMGLLM TGGDHGTARV VQAALVIGDA
     IEQEVRIHNF LEKTKKQKAE KDKQKEDGEH VTQEQEKLRK KVTNLMKKQK LRAVGQIVRR
     QDDSKPWGQD AKAKVGSRLI ELLLQTAYIQ PPANQLAVDP PDIRPAFLHS VRTVAKETKS
     ASRRYGIIQC DELVFKGLER TARHMVIPYM PMLVPPVKWT GYDKGGHLYL PSYVMRTHGA
     RQQREAVKRA SRNQLQPVFE ALDTLGSTKW RINKRVLSVI DRIWAGGGRL ADLVDRDDAP
     LPEEPDTEDE ALRTKWRWKV KSVKKENRER HSQRCDIELK LAVARKMKDE EGFFYPHNVD
     FRGRAYPMHP HLNHLGSDIC RGVLVFAEGR PLGESGLRWL KIHLANLFAG GVEKLSLEGR
     IAFTENHMDD IFDSADKPLE GRRWWLNAED PFQCLAVCIN LSEAVRSSSP ETSISHIPVH
     QDGSCNGLQH YAALGRDELG AAAVNLVAGE KPADVYSGIA ARVLDIMKRD AQRDPAEFPD
     AVRARALVNQ VDRKLVKQTV MTSVYGVTYI GARDQIKRRL KERGAIADDS ELFGAACYAA
     KVTLTALGEM FEAARSIMTW LGECAKIIAS ENEPVRWTTP LGLPVVQPYR KIGRHLIKTS
     LQILTLQQET EKVMVKRQRT AFPPNFIHSL DGSHMMMTAV ACRRAGLNFA GVHDSYWTHA
     CDVDKLNRIL REKFVELYET PILEKLLESF QTSYPTLLFP PLPERGDFDL RDVLESPYFF
     N