ID   RPO2N_METJA             Reviewed;         498 AA.
AC   Q58444;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=DNA-directed RNA polymerase subunit Rpo2N {ECO:0000305};
DE            EC=2.7.7.6 {ECO:0000250|UniProtKB:P11513};
DE   AltName: Full=DNA-directed RNA polymerase subunit B'' {ECO:0000303|PubMed:8688087};
GN   Name=rpo2N {ECO:0000305}; Synonyms=rpoB2 {ECO:0000303|PubMed:8688087};
GN   OrderedLocusNames=MJ1040;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC       transcription of DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this
CC       organism) is implicated in DNA promoter recognition and in nucleotide
CC       binding. {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000250|UniProtKB:P11513};
CC   -!- SUBUNIT: Part of the RNA polymerase complex.
CC       {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; L77117; AAB99043.1; -; Genomic_DNA.
DR   PIR; G64429; G64429.
DR   AlphaFoldDB; Q58444; -.
DR   SMR; Q58444; -.
DR   STRING; 243232.MJ_1040; -.
DR   EnsemblBacteria; AAB99043; AAB99043; MJ_1040.
DR   KEGG; mja:MJ_1040; -.
DR   eggNOG; arCOG01762; Archaea.
DR   HOGENOM; CLU_000524_5_3_2; -.
DR   InParanoid; Q58444; -.
DR   OMA; GKICPSE; -.
DR   PhylomeDB; Q58444; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transcription; Transferase.
FT   CHAIN           1..498
FT                   /note="DNA-directed RNA polymerase subunit Rpo2N"
FT                   /id="PRO_0000074034"
SQ   SEQUENCE   498 AA;  56487 MW;  F57FD38EEEB79298 CRC64;
     MAIMRELVDA YFKEHGLIDH QIESYNDFVE NRLQKIIDEV GYIETEITGG YKVKLGKIKV
     GKPVIKEADG SIRPITPMEA RIRDLTYSVP LYLEMTPIIG EGEDAREGET VEVYIGELPV
     MLGSKICHLY GKSREELIDL GEDPEDPFGY FIINGTEKVL ITQEDLIPNR ILCEKAERSG
     KIVDVAKVFS TRHGFRALCT VERHPDGLLY ATFPGMPGQI PLVILMKALG AETDKDIIES
     IDDERFFMEI VLNIQEIREE HNINSPEDAL EFIGKRVAPG QAKDYRLKRA ETVLCNYLLP
     HLGVTKEDFP KKIRFLGIMA RNALELYFGY RGEDDKDHYA YKRAKLAGDL MEDLFRYAFS
     QLVKDIKYQL ERQTLRNKTP SIQAAVRSDI LTERIKHAMA TGTWVGGKTG VSQLLDRTSY
     LATNSQLRRI VSPLSRSQPH FEARELHGTH WGKICPSETP EGPNCGLVKN FAIMCKVTRE
     EDDSKVIELL KSFGINVS