ID   RPO2N_METTH             Reviewed;         519 AA.
AC   O27123;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=DNA-directed RNA polymerase subunit Rpo2N {ECO:0000305};
DE            EC=2.7.7.6 {ECO:0000250|UniProtKB:P11513};
DE   AltName: Full=DNA-directed RNA polymerase subunit B'' {ECO:0000303|PubMed:9371463};
GN   Name=rpo2N {ECO:0000305}; Synonyms=rpoB2; OrderedLocusNames=MTH_1049;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC       transcription of DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this
CC       organism) is implicated in DNA promoter recognition and in nucleotide
CC       binding. {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000250|UniProtKB:P11513};
CC   -!- SUBUNIT: Part of the RNA polymerase complex.
CC       {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; AE000666; AAB85540.1; -; Genomic_DNA.
DR   PIR; D69006; D69006.
DR   AlphaFoldDB; O27123; -.
DR   SMR; O27123; -.
DR   STRING; 187420.MTH_1049; -.
DR   EnsemblBacteria; AAB85540; AAB85540; MTH_1049.
DR   KEGG; mth:MTH_1049; -.
DR   PATRIC; fig|187420.15.peg.1028; -.
DR   HOGENOM; CLU_000524_5_3_2; -.
DR   OMA; GKICPSE; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transcription; Transferase.
FT   CHAIN           1..519
FT                   /note="DNA-directed RNA polymerase subunit Rpo2N"
FT                   /id="PRO_0000074032"
SQ   SEQUENCE   519 AA;  59191 MW;  F82AA2C85F6FC6D0 CRC64;
     MFWRKSMKKS AWGLVDAFFD KYDLVDHHIH SYNDFVSNRI QEIIDTSEPI ELEQGKYRVE
     TGKVSIEKPF IKEADGSKSK IYPTEARLRN LTYSAHMSLE MRLLKEGGPE TEFEKVYIGE
     LPVMLKSEIC HLHGLSRKEL MEKGEDPADP GGYFIVNGSE RSIVTMEEIA PNKIILERIG
     EEDENRARAI VTSIRSGFRA RISLEYRKPR KTGVFLRISF PYVPGEIPLV ILLRALGLAT
     DQEIITSISD DFNYQMIAAD DIQVSLDRLN LNKKEMKELD EEDRREYLIR SAIKYIGNRV
     AKGMTEDYRI KRAEDVIDRY LLPHIGTEPE KRLEKATYLA EMTEMLLQVI SGERRPHDKD
     HYTNKRLRVS GDLMEDLLRV AFTSLSRDMS YQLERSLARG KEPSVKQAVR SDVLTENLKH
     AIATGNWVGG RAGVSQLLDR TSYMGTLSHM RRVVSPLSRS QPHFEARDLH PTQFGKICPN
     ETPEGPNCGL VKNLALLAKI SEGSDASEVE EVIKMGVVN