ID   RPO2N_METVS             Reviewed;         499 AA.
AC   P41558; A6UQ05;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=DNA-directed RNA polymerase subunit Rpo2N {ECO:0000305};
DE            EC=2.7.7.6 {ECO:0000250|UniProtKB:P11513};
DE   AltName: Full=DNA-directed RNA polymerase subunit B'' {ECO:0000303|Ref.1};
GN   Name=rpo2N {ECO:0000305}; Synonyms=rpoB2; OrderedLocusNames=Mevan_0671;
OS   Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148
OS   / SB).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=406327;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Palm P., Arnold-Ammer I., Lechner K.A., Zillig W.;
RT   "DNA sequence of the genes of the large subunits of the DNA dependent RNA-
RT   polymerase of Methanococcus vannielii.";
RL   Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Anderson I.,
RA   Sieprawska-Lupa M., Whitman W.B., Richardson P.;
RT   "Complete sequence of Methanococcus vannielii SB.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC       transcription of DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this
CC       organism) is implicated in DNA promoter recognition and in nucleotide
CC       binding. {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000250|UniProtKB:P11513};
CC   -!- SUBUNIT: Part of the RNA polymerase complex.
CC       {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B8YB55}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; X73293; CAA51726.1; -; Genomic_DNA.
DR   EMBL; CP000742; ABR54577.1; -; Genomic_DNA.
DR   PIR; S47160; S47160.
DR   RefSeq; WP_011972479.1; NC_009634.1.
DR   AlphaFoldDB; P41558; -.
DR   SMR; P41558; -.
DR   STRING; 406327.Mevan_0671; -.
DR   EnsemblBacteria; ABR54577; ABR54577; Mevan_0671.
DR   GeneID; 5325115; -.
DR   KEGG; mvn:Mevan_0671; -.
DR   eggNOG; arCOG01762; Archaea.
DR   HOGENOM; CLU_000524_5_3_2; -.
DR   OMA; GKICPSE; -.
DR   OrthoDB; 14180at2157; -.
DR   Proteomes; UP000001107; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Transcription; Transferase.
FT   CHAIN           1..499
FT                   /note="DNA-directed RNA polymerase subunit Rpo2N"
FT                   /id="PRO_0000074035"
SQ   SEQUENCE   499 AA;  55900 MW;  96B5672C259DC2C2 CRC64;
     MESRVIVDAF FRENSLVKHH IDSYDDFVEN KIQGIIDEVT GVETEIKGGY KVSFGKVRVT
     KPINKEADGS VKEITPMEAR IRNLAYSAPL YLEMIPLIGE GDEEKTLSPI EVYIGELPVM
     LGAKICHLSG KSEEDMINYG EDPKDPLGYF IVNGSEKAVV AQEDLIPNRI LCEKVEKNNK
     IVDIAKVFST RHGFRALCTV ERSPDGLLNV SFPGMPSTIP LVILMRALGA ESDREIMELI
     SDEPTVVMQL VANLQEAREE HGINTTEDAL EHIGKRVAPG QPKEYKLKRA ETILCNYLLP
     HMGIESEKLG AKCKYLGRMA KNSIELYLGS RVEDDKDHYA NKRLKLAGDL MEDLFRHSFN
     QLIKDIKYQL ERQAIRNKEP SIQAAVRSDV LTERMRHAMA TGNWVGGRTG VSQLLDRTSY
     LATVSQLRRV VSPLSRSQPH FEARDLHATQ WGKICPSETP EGPNCGLVKN LAVMCKVTTD
     EEDEGIIQLI KEIGLSKDI