RPO2_SULAC
ID RPO2_SULAC Reviewed; 1126 AA.
AC P11513; Q4JAV6;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=DNA-directed RNA polymerase subunit Rpo2 {ECO:0000305};
DE EC=2.7.7.6 {ECO:0000269|Ref.4};
DE AltName: Full=DNA-directed RNA polymerase subunit B {ECO:0000303|PubMed:2501756};
GN Name=rpo2 {ECO:0000305};
GN Synonyms=rpoB {ECO:0000303|PubMed:1729711},
GN rpoB' {ECO:0000303|PubMed:2501756}; OrderedLocusNames=Saci_0693;
OS Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS 15157 / NCIMB 11770).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfolobus.
OX NCBI_TaxID=330779;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=2501756; DOI=10.1093/nar/17.12.4517;
RA Puehler G., Lottspeich F., Zillig W.;
RT "Organization and nucleotide sequence of the genes encoding the large
RT subunits A, B and C of the DNA-dependent RNA polymerase of the
RT archaebacterium Sulfolobus acidocaldarius.";
RL Nucleic Acids Res. 17:4517-4534(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT "The genome of Sulfolobus acidocaldarius, a model organism of the
RT Crenarchaeota.";
RL J. Bacteriol. 187:4992-4999(2005).
RN [3]
RP SUBUNIT.
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=1729711; DOI=10.1073/pnas.89.1.407;
RA Klenk H.-P., Palm P., Lottspeich F., Zillig W.;
RT "Component H of the DNA-dependent RNA polymerases of Archaea is homologous
RT to a subunit shared by the three eucaryal nuclear RNA polymerases.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:407-410(1992).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, COFACTOR, AND SUBUNIT.
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX DOI=10.1016/S0723-2020(11)80337-1;
RA Lanzendorfer M., Langer D., Hain J., Klenk H.-P., Holz I., Arnold-Ammer I.,
RA Zillig W.;
RT "Structure and Function of the DNA-Dependent RNA Polymerase of
RT Sulfolobus.";
RL Syst. Appl. Microbiol. 16:656-664(1994).
CC -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC transcription of DNA into RNA using the four ribonucleoside
CC triphosphates as substrates (Ref.4). This subunit is involved in DNA
CC promoter recognition (By similarity). {ECO:0000250|UniProtKB:B8YB55,
CC ECO:0000269|Ref.4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000269|Ref.4};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305|Ref.4};
CC Note=Binds 1 Zn(2+) per subunit. {ECO:0000305|Ref.4};
CC -!- SUBUNIT: Part of the 13-subunit RNA polymerase complex.
CC {ECO:0000269|PubMed:1729711, ECO:0000269|Ref.4}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:B8YB55}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000305|PubMed:2501756}.
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DR EMBL; X14818; CAA32924.1; -; Genomic_DNA.
DR EMBL; CP000077; AAY80073.1; -; Genomic_DNA.
DR PIR; S04716; S04716.
DR RefSeq; WP_011277575.1; NC_007181.1.
DR PDB; 7OK0; EM; 2.90 A; B=1-1126.
DR PDB; 7OQ4; EM; 3.27 A; B=1-1126.
DR PDB; 7OQY; EM; 2.61 A; B=1-1126.
DR PDBsum; 7OK0; -.
DR PDBsum; 7OQ4; -.
DR PDBsum; 7OQY; -.
DR AlphaFoldDB; P11513; -.
DR SMR; P11513; -.
DR STRING; 330779.Saci_0693; -.
DR EnsemblBacteria; AAY80073; AAY80073; Saci_0693.
DR GeneID; 3472539; -.
DR KEGG; sai:Saci_0693; -.
DR PATRIC; fig|330779.12.peg.661; -.
DR eggNOG; arCOG01762; Archaea.
DR HOGENOM; CLU_000524_5_1_2; -.
DR OMA; RDLHGTH; -.
DR Proteomes; UP000001018; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IDA:UniProtKB.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR Gene3D; 2.40.270.10; -; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1110.10; -; 1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007646; RNA_pol_Rpb2_4.
DR InterPro; IPR007647; RNA_pol_Rpb2_5.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR InterPro; IPR019969; RNAP_Rpo2.
DR PANTHER; PTHR20856; PTHR20856; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF04566; RNA_pol_Rpb2_4; 1.
DR Pfam; PF04567; RNA_pol_Rpb2_5; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR TIGRFAMs; TIGR03670; rpoB_arch; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-binding; DNA-directed RNA polymerase;
KW Metal-binding; Nucleotidyltransferase; Reference proteome; Transcription;
KW Transferase; Zinc.
FT CHAIN 1..1126
FT /note="DNA-directed RNA polymerase subunit Rpo2"
FT /id="PRO_0000048097"
FT BINDING 1060
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:B8YB55"
FT BINDING 1063
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:B8YB55"
FT BINDING 1078
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:B8YB55"
FT BINDING 1081
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:B8YB55"
FT CONFLICT 158
FT /note="V -> M (in Ref. 1; CAA32924)"
FT /evidence="ECO:0000305"
FT CONFLICT 1053
FT /note="D -> Y (in Ref. 1; CAA32924)"
FT /evidence="ECO:0000305"
FT HELIX 4..17
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 22..43
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 49..52
FT /evidence="ECO:0007829|PDB:7OQ4"
FT STRAND 53..62
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 64..67
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 69..71
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 80..86
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 91..104
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 110..118
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 123..126
FT /evidence="ECO:0007829|PDB:7OQ4"
FT HELIX 127..129
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 133..138
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 150..152
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 155..159
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 161..165
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 167..169
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 171..174
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 179..190
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 195..203
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 204..206
FT /evidence="ECO:0007829|PDB:7OQ4"
FT STRAND 208..212
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 221..226
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 227..229
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 233..240
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 244..247
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 248..250
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 251..256
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 263..274
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 282..291
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 297..299
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 303..305
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 306..324
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 333..335
FT /evidence="ECO:0007829|PDB:7OK0"
FT HELIX 336..338
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 339..342
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 344..368
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 370..372
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 379..382
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 388..398
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 402..404
FT /evidence="ECO:0007829|PDB:7OK0"
FT STRAND 409..411
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 417..423
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 426..429
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 438..441
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 445..447
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 448..450
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 460..464
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 465..468
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 480..489
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 495..503
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 504..506
FT /evidence="ECO:0007829|PDB:7OK0"
FT HELIX 509..512
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 515..520
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 523..529
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 531..543
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 552..557
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 562..567
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 573..581
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 584..586
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 589..596
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 602..607
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 610..614
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 618..621
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 624..627
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 628..630
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 637..640
FT /evidence="ECO:0007829|PDB:7OQ4"
FT HELIX 642..645
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 650..652
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 653..655
FT /evidence="ECO:0007829|PDB:7OQ4"
FT HELIX 656..658
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 661..670
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 672..674
FT /evidence="ECO:0007829|PDB:7OQ4"
FT HELIX 682..684
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 688..695
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 700..702
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 704..708
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 709..713
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 718..725
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 728..731
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 733..739
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 740..744
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 745..748
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 750..757
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 765..767
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 771..773
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 784..786
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 794..796
FT /evidence="ECO:0007829|PDB:7OK0"
FT STRAND 807..809
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 811..813
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 843..852
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 854..856
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 858..868
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 875..878
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 883..890
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 892..894
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 905..908
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 910..912
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 914..916
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 919..934
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 949..958
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 965..970
FT /evidence="ECO:0007829|PDB:7OK0"
FT TURN 972..974
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 982..993
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 996..998
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 1001..1005
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 1010..1012
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 1018..1022
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 1025..1027
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 1029..1038
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 1041..1048
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 1049..1052
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 1054..1060
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 1061..1063
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 1068..1070
FT /evidence="ECO:0007829|PDB:7OK0"
FT STRAND 1071..1073
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 1076..1078
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 1079..1081
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 1087..1093
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 1094..1105
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 1111..1118
FT /evidence="ECO:0007829|PDB:7OQY"
SQ SEQUENCE 1126 AA; 126464 MW; F45F6B182532EEC7 CRC64;
MLDTESRWAI AESFFKTRGL VRQHLDSFND FLRNKLQQVI YEQGEIVTEV PGLKIKLGKI
RYEKPSIRET DKGPMREITP MEARLRNLTY SSPIFLSMIP VENNIEGEPI EIYIGDLPIM
LKSVADPTSN LPIDKLIEIG EDPKDPGGYF IVNGSEKVII AQEDLATNRV LVDYGKSGSN
ITHVAKVTSS AAGYRVQVMI ERLKDSTIQI SFATVPGRIP FAIIMRALGF VTDRDIVYAV
SLDPQIQNEL LPSLEQASSI TSAEEALDFI GNRVAIGQKR ENRIQKAEQV IDKYFLPHLG
TSPEDRKKKG YYLASAVNKI LELYLGRREP DDKDHYANKR VRLAGDLFTS LFRVAFKAFV
KDLVYQLEKS KVRGRRLSLT ALVRADIITE RIRHALATGN WVGGRTGVSQ LLDRTNWLSM
LSHLRRVVSS LARGQPNFEA RDLHGTQWGR MCPFETPEGP NSGLVKNLAL LAQVSVGINE
SVVERVAYEL GVVSVEDVIR RISEQNEDVE KYMSWSKVYL NGRLLGYYED GKELAKKIRE
SRRQGKLSDE VNVAYIATDY LNEVHINCDA GRVRRPLIIV NNGTPLVDTE DIKKLKNGEI
TFDDLVKQGK IEFIDAEEEE NAYVALNPQD LTPDHTHLEI WPSAILGIIA SIIPYPEHNQ
SPRNTYQSAM AKQSLGLYAS NYQIRTDTRA HLLHYPQMPL VQTRMLGVIG YNDRPAGANA
ILAIMSYTGY NMEDSIIMNK SSIERGMYRS TFFRLYSTEE VKYPGGQEDK IVTPEAGVKG
YKGKDYYRLL EDNGVVSPEV EVKGGDVLIG KVSPPRFLQE FKELSPEQAK RDTSIVTRHG
ENGIVDLVLI TETLEGNKLV KVRVRDLRIP EIGDKFATRH GQKGVVGILI DQVDMPYTAK
GIVPDIILNP HALPSRMTIG QIMEAIGGKY AALSGKPVDA TPFLETPKLQ EMQKEILKLG
HLPDSTEVVY DGRTGQKLKS RILFGIVYYQ KLHHMVADKM HARARGPVQI LTRQPTEGRA
REGGLRFGEM ERDCLIGFGT AMLIKDRLLD NSDKAVVYIC DQCGYVGWYD RSKNRYVCPV
HGDKSVLHPV TVSYAFKLLI QELMSMVISP RLILGEKVNL GGASNE
