ID   RPO3B_TOBAC             Reviewed;         977 AA.
AC   Q8L6J1; Q8L6J6;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 2.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=DNA-directed RNA polymerase 3B, chloroplastic;
DE            EC=2.7.7.6;
DE   AltName: Full=NictaRpoT3-tom;
DE   AltName: Full=T7 bacteriophage-type single subunit RNA polymerase 3B;
DE   Flags: Precursor;
GN   Name=RPOT3-TOM;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND SUBCELLULAR LOCATION.
RX   PubMed=12061895; DOI=10.1046/j.1365-313x.2002.01318.x;
RA   Hedtke B., Legen J., Weihe A., Herrmann R.G., Boerner T.;
RT   "Six active phage-type RNA polymerase genes in Nicotiana tabacum.";
RL   Plant J. 30:625-637(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|PROSITE-ProRule:PRU10031,
CC         ECO:0000255|PROSITE-ProRule:PRU10032};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000269|PubMed:12061895}.
CC   -!- SIMILARITY: Belongs to the phage and mitochondrial RNA polymerase
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC95026.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AJ416570; CAC95021.2; -; mRNA.
DR   EMBL; AJ416575; CAC95026.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; NP_001312292.1; NM_001325363.1.
DR   AlphaFoldDB; Q8L6J1; -.
DR   SMR; Q8L6J1; -.
DR   GeneID; 107783504; -.
DR   KEGG; nta:107783504; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0034245; C:mitochondrial DNA-directed RNA polymerase complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IBA:GO_Central.
DR   GO; GO:0006390; P:mitochondrial transcription; IBA:GO_Central.
DR   Gene3D; 1.10.1320.10; -; 1.
DR   Gene3D; 1.10.287.260; -; 1.
DR   InterPro; IPR024075; DNA-dir_RNA_pol_helix_hairp_sf.
DR   InterPro; IPR002092; DNA-dir_Rpol_phage-type.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR037159; RNA_POL_N_sf.
DR   InterPro; IPR029262; RPOL_N.
DR   PANTHER; PTHR10102; PTHR10102; 1.
DR   Pfam; PF00940; RNA_pol; 1.
DR   Pfam; PF14700; RPOL_N; 1.
DR   SMART; SM01311; RPOL_N; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS00900; RNA_POL_PHAGE_1; 1.
DR   PROSITE; PS00489; RNA_POL_PHAGE_2; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Reference proteome; Transcription; Transferase; Transit peptide.
FT   TRANSIT         1..71
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           72..977
FT                   /note="DNA-directed RNA polymerase 3B, chloroplastic"
FT                   /id="PRO_0000046035"
FT   ACT_SITE        678
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        753
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        910
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   977 AA;  111548 MW;  C4572496EA4A03C7 CRC64;
     MASTASYSPS PTSQWRTQKL PKRFNFYVIH NQEFGKLSQS SSLSTSSFPK TLKLPVIHMP
     INNIQSQTTV CVSTDENLEE LVNLQKIPNG FLNKESNKRV FIQDPPWVSS LFMNSLFVRA
     KQVQGVRREF REIERRRRYA MLRRRQIKAE TEAWEQMVEE YRELEREMCE KKLAPNLPYV
     KKLLLGWFEP LRQAIEKEQN AETTVKHRAA FAPHIDSLPA DKMAVIVMHK LMGLLMMGGK
     EERCVQVVQA AVQIGMAVEN EVRIHNFLEK TKKLQKHMTG AQSQEDMSKE TMILRKRVKS
     LIKRNRVVEV RKLMKSEEPE SWGRDTQAKL GCRLLELLTE TAYVQRPVDQ SADTPPDIRP
     AFRHVFKIAT RDPGKNIVKK YGVIECDPLV VAGVDRTVKQ MMIPYVPMLV PPKKWRGYDK
     GGYLFLPSYL MRTHGSRRQQ DAVRGVPTKQ MQQVYEALDT LGSTKWRVNK RILNVVESIW
     AGGGNIAGLV DRKDVPIPEL SNSDDIMEVK KWKWKVRKSK KINQELHSQR CDTELKLSVA
     RKLKDEEGFY YPHNLDFRGR AYPMHPHLNH LSSDLCRGIL EFAEGRPLGK SGLRWLKIHL
     ASLYAGGVEK LCYDARLAFV ENHIHDILDS ANNPLNGNRW WLNAEDPFQC LAACINLSEA
     LKSSSPHTVI SHLPIHQDGS CNGLQHYAAL GRDSMEAAAV NLVAGEKPAD VYTEIALRVD
     HIIRGDSIKD PAIDPNALLA KLLIDQVDRK LVKQTVMTSV YGVTYVGARE QIKRRLEEKG
     LIDDDRLLFT ASCYAAKVTL AALGELFQAA RGTMTWLGDC AKVIALENQP VRWTTPLGLP
     VVQPYFKTQR HVIRTSLQIL ALQREGDAVE VRKQRTAFPP NFVHSLDGSH MMMTAVACRD
     AGLQFAGVHD SFWTHACDVD QMNRILREKF VELYSLPILE DLLENFQKSY PALTFPPLPK
     RGDFNLREVL ESPYFFN