RPO3_HALSA
ID RPO3_HALSA Reviewed; 248 AA.
AC Q9HQJ4; Q9V2W1;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2001, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=DNA-directed RNA polymerase subunit Rpo3 {ECO:0000255|HAMAP-Rule:MF_00320};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00320};
DE AltName: Full=DNA-directed RNA polymerase subunit D {ECO:0000255|HAMAP-Rule:MF_00320};
GN Name=rpo3 {ECO:0000255|HAMAP-Rule:MF_00320};
GN Synonyms=rpoD {ECO:0000255|HAMAP-Rule:MF_00320};
GN OrderedLocusNames=VNG_1136G;
OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS (Halobacterium halobium).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halobacterium.
OX NCBI_TaxID=64091;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=R1 / S9;
RX PubMed=10527834; DOI=10.1006/bbrc.1999.1480;
RA Sano K., Taguchi A., Furumoto H., Uda T., Itoh T.;
RT "Cloning, sequencing, and characterization of ribosomal protein and RNA
RT polymerase genes from the region analogous to the alpha-operon of
RT Escherichia coli in halophilic archaea, Halobacterium halobium.";
RL Biochem. Biophys. Res. Commun. 264:24-28(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX PubMed=11016950; DOI=10.1073/pnas.190337797;
RA Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA DasSarma S.;
RT "Genome sequence of Halobacterium species NRC-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC transcription of DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. {ECO:0000255|HAMAP-Rule:MF_00320}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00320};
CC -!- SUBUNIT: Part of the RNA polymerase complex. {ECO:0000255|HAMAP-
CC Rule:MF_00320}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00320}.
CC -!- SIMILARITY: Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase
CC subunit family. {ECO:0000255|HAMAP-Rule:MF_00320}.
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DR EMBL; AB030282; BAA85898.1; -; Genomic_DNA.
DR EMBL; AE004437; AAG19520.1; -; Genomic_DNA.
DR PIR; D84269; D84269.
DR PIR; T43940; T43940.
DR RefSeq; WP_010902815.1; NC_002607.1.
DR AlphaFoldDB; Q9HQJ4; -.
DR SMR; Q9HQJ4; -.
DR STRING; 64091.VNG_1136G; -.
DR PaxDb; Q9HQJ4; -.
DR EnsemblBacteria; AAG19520; AAG19520; VNG_1136G.
DR GeneID; 5954259; -.
DR GeneID; 62886654; -.
DR KEGG; hal:VNG_1136G; -.
DR PATRIC; fig|64091.14.peg.866; -.
DR HOGENOM; CLU_038421_3_1_2; -.
DR InParanoid; Q9HQJ4; -.
DR OMA; ECLRHPE; -.
DR OrthoDB; 72726at2157; -.
DR PhylomeDB; Q9HQJ4; -.
DR Proteomes; UP000000554; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 2.170.120.12; -; 1.
DR Gene3D; 3.30.1360.10; -; 1.
DR HAMAP; MF_00320; RNApol_arch_Rpo3; 1.
DR InterPro; IPR001514; DNA-dir_RNA_pol_30-40kDasu_CS.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR022842; RNAP_Rpo3/Rpb3/RPAC1.
DR InterPro; IPR036643; RNApol_insert_sf.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF55257; SSF55257; 1.
DR SUPFAM; SSF56553; SSF56553; 1.
DR PROSITE; PS00446; RNA_POL_D_30KD; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..248
FT /note="DNA-directed RNA polymerase subunit Rpo3"
FT /id="PRO_0000132753"
FT CONFLICT 79
FT /note="A -> P (in Ref. 1; BAA85898)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="Missing (in Ref. 1; BAA85898)"
FT /evidence="ECO:0000305"
FT CONFLICT 98
FT /note="S -> P (in Ref. 1; BAA85898)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="M -> D (in Ref. 1; BAA85898)"
FT /evidence="ECO:0000305"
FT CONFLICT 155
FT /note="Q -> QLQ (in Ref. 1; BAA85898)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 248 AA; 27377 MW; C2067E1FF3C6A25C CRC64;
MSSQDFDVEF VDRDDREARF VVRNITPAFA NGIRRAILVD VPTLSIDTVR FVENSSVMFD
EQLGLRLGLV PLTTPEDYAA GEAVTLALDV EGPGTAYSGD LVSNDPEVEA ADENIPIIEL
KDDQRLELEA DAVMGHGRDH AKHQGGVAVG YRHLQRVHVV GDSPEYADDD PQMLRGVIEE
DDELVPTDDF DNDLTTRYPG KEVEIEDVDG AFVFHVESDG SMPVEELVLR AVDTLVDRAD
ELEQAVQL
