ID   RPO5_SACS2              Reviewed;          84 AA.
AC   Q980Q9;
DT   03-APR-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=DNA-directed RNA polymerase subunit Rpo5 {ECO:0000255|HAMAP-Rule:MF_00025};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00025};
DE   AltName: Full=DNA-directed RNA polymerase subunit H {ECO:0000255|HAMAP-Rule:MF_00025};
GN   Name=rpo5 {ECO:0000255|HAMAP-Rule:MF_00025};
GN   Synonyms=rpoH {ECO:0000255|HAMAP-Rule:MF_00025,
GN   ECO:0000303|PubMed:11427726}; OrderedLocusNames=SSO5468;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN   [2] {ECO:0007744|PDB:2PMZ, ECO:0007744|PDB:3HKZ}
RP   X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF THE RNA POLYMERASE COMPLEX, AND
RP   SUBUNIT.
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=18235446; DOI=10.1038/nature06530;
RA   Hirata A., Klein B.J., Murakami K.S.;
RT   "The X-ray crystal structure of RNA polymerase from Archaea.";
RL   Nature 451:851-854(2008).
CC   -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC       transcription of DNA into RNA using the four ribonucleoside
CC       triphosphates as substrates. {ECO:0000255|HAMAP-Rule:MF_00025}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00025};
CC   -!- SUBUNIT: Part of the 13-subunit RNA polymerase complex.
CC       {ECO:0000269|PubMed:18235446}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00025}.
CC   -!- SIMILARITY: Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase
CC       subunit family. {ECO:0000255|HAMAP-Rule:MF_00025}.
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DR   EMBL; AE006641; AAK40569.1; -; Genomic_DNA.
DR   PIR; B90164; B90164.
DR   RefSeq; WP_009990480.1; NC_002754.1.
DR   PDB; 2PMZ; X-ray; 3.40 A; H/V=1-84.
DR   PDB; 3HKZ; X-ray; 3.40 A; H/T=1-84.
DR   PDBsum; 2PMZ; -.
DR   PDBsum; 3HKZ; -.
DR   AlphaFoldDB; Q980Q9; -.
DR   SMR; Q980Q9; -.
DR   DIP; DIP-60641N; -.
DR   IntAct; Q980Q9; 1.
DR   STRING; 273057.SSO5468; -.
DR   EnsemblBacteria; AAK40569; AAK40569; SSO5468.
DR   GeneID; 44129197; -.
DR   KEGG; sso:SSO5468; -.
DR   PATRIC; fig|273057.12.peg.224; -.
DR   eggNOG; arCOG04258; Archaea.
DR   HOGENOM; CLU_058320_4_0_2; -.
DR   InParanoid; Q980Q9; -.
DR   OMA; PKIYHDD; -.
DR   PhylomeDB; Q980Q9; -.
DR   BRENDA; 2.7.7.6; 6163.
DR   EvolutionaryTrace; Q980Q9; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.90.940.20; -; 1.
DR   HAMAP; MF_00025; RNApol_Rpo5_RPB5; 1.
DR   InterPro; IPR014381; Arch_Rpo5/euc_Rpb5.
DR   InterPro; IPR000783; RNA_pol_subH/Rpb5_C.
DR   InterPro; IPR020608; RNA_pol_subH/Rpb5_CS.
DR   InterPro; IPR035913; RPB5-like_sf.
DR   PANTHER; PTHR10535; PTHR10535; 1.
DR   Pfam; PF01191; RNA_pol_Rpb5_C; 1.
DR   SUPFAM; SSF55287; SSF55287; 1.
DR   PROSITE; PS01110; RNA_POL_H_23KD; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; DNA-directed RNA polymerase;
KW   Nucleotidyltransferase; Reference proteome; Transcription; Transferase.
FT   CHAIN           1..84
FT                   /note="DNA-directed RNA polymerase subunit Rpo5"
FT                   /id="PRO_0000146104"
FT   STRAND          14..16
FT                   /evidence="ECO:0007829|PDB:2PMZ"
FT   TURN            25..27
FT                   /evidence="ECO:0007829|PDB:2PMZ"
FT   HELIX           28..34
FT                   /evidence="ECO:0007829|PDB:2PMZ"
FT   STRAND          41..43
FT                   /evidence="ECO:0007829|PDB:2PMZ"
FT   TURN            50..56
FT                   /evidence="ECO:0007829|PDB:2PMZ"
FT   STRAND          59..61
FT                   /evidence="ECO:0007829|PDB:3HKZ"
FT   STRAND          63..67
FT                   /evidence="ECO:0007829|PDB:2PMZ"
FT   STRAND          70..73
FT                   /evidence="ECO:0007829|PDB:2PMZ"
FT   STRAND          75..78
FT                   /evidence="ECO:0007829|PDB:2PMZ"
SQ   SEQUENCE   84 AA;  9670 MW;  2873A2053F864712 CRC64;
     MRGSSNKKID PRIHYLVPKH EVLNIDEAYK ILKELGIRPE QLPWIRASDP VARSINAKPG
     DIIRIIRKSQ LYGEVVSYRY VISG