RPO5_SULAC
ID RPO5_SULAC Reviewed; 84 AA.
AC P11521; Q4JAV5;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=DNA-directed RNA polymerase subunit Rpo5 {ECO:0000255|HAMAP-Rule:MF_00025};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00025, ECO:0000269|Ref.4};
DE AltName: Full=DNA-directed RNA polymerase subunit H {ECO:0000255|HAMAP-Rule:MF_00025};
GN Name=rpo5 {ECO:0000255|HAMAP-Rule:MF_00025};
GN Synonyms=rpoB'' {ECO:0000303|PubMed:2501756},
GN rpoH {ECO:0000255|HAMAP-Rule:MF_00025, ECO:0000303|PubMed:1729711};
GN OrderedLocusNames=Saci_0694;
OS Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS 15157 / NCIMB 11770).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Sulfolobus.
OX NCBI_TaxID=330779;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=2501756; DOI=10.1093/nar/17.12.4517;
RA Puehler G., Lottspeich F., Zillig W.;
RT "Organization and nucleotide sequence of the genes encoding the large
RT subunits A, B and C of the DNA-dependent RNA polymerase of the
RT archaebacterium Sulfolobus acidocaldarius.";
RL Nucleic Acids Res. 17:4517-4534(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT "The genome of Sulfolobus acidocaldarius, a model organism of the
RT Crenarchaeota.";
RL J. Bacteriol. 187:4992-4999(2005).
RN [3]
RP PROTEIN SEQUENCE OF 1-8, AND SUBUNIT.
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX PubMed=1729711; DOI=10.1073/pnas.89.1.407;
RA Klenk H.-P., Palm P., Lottspeich F., Zillig W.;
RT "Component H of the DNA-dependent RNA polymerases of Archaea is homologous
RT to a subunit shared by the three eucaryal nuclear RNA polymerases.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:407-410(1992).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBUNIT.
RC STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX DOI=10.1016/S0723-2020(11)80337-1;
RA Lanzendorfer M., Langer D., Hain J., Klenk H.-P., Holz I., Arnold-Ammer I.,
RA Zillig W.;
RT "Structure and Function of the DNA-Dependent RNA Polymerase of
RT Sulfolobus.";
RL Syst. Appl. Microbiol. 16:656-664(1994).
CC -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC transcription of DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. {ECO:0000255|HAMAP-Rule:MF_00025}.
CC -!- FUNCTION: Reconstitution experiments show this subunit is required for
CC basic activity. {ECO:0000269|Ref.4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00025,
CC ECO:0000269|Ref.4};
CC -!- SUBUNIT: Part of the 13-subunit RNA polymerase.
CC {ECO:0000269|PubMed:1729711, ECO:0000269|Ref.4}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00025}.
CC -!- SIMILARITY: Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase
CC subunit family. {ECO:0000255|HAMAP-Rule:MF_00025,
CC ECO:0000303|PubMed:1729711}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA32923.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X14818; CAA32923.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP000077; AAY80074.1; -; Genomic_DNA.
DR PIR; S04715; S04715.
DR RefSeq; WP_011277576.1; NC_007181.1.
DR PDB; 7OK0; EM; 2.90 A; H=1-84.
DR PDB; 7OQ4; EM; 3.27 A; H=1-84.
DR PDB; 7OQY; EM; 2.61 A; H=1-84.
DR PDBsum; 7OK0; -.
DR PDBsum; 7OQ4; -.
DR PDBsum; 7OQY; -.
DR AlphaFoldDB; P11521; -.
DR SMR; P11521; -.
DR STRING; 330779.Saci_0694; -.
DR EnsemblBacteria; AAY80074; AAY80074; Saci_0694.
DR GeneID; 3472540; -.
DR KEGG; sai:Saci_0694; -.
DR PATRIC; fig|330779.12.peg.662; -.
DR eggNOG; arCOG04258; Archaea.
DR HOGENOM; CLU_058320_4_0_2; -.
DR OMA; PKIYHDD; -.
DR BRENDA; 2.7.7.6; 6160.
DR Proteomes; UP000001018; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IDA:UniProtKB.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 3.90.940.20; -; 1.
DR HAMAP; MF_00025; RNApol_Rpo5_RPB5; 1.
DR InterPro; IPR014381; Arch_Rpo5/euc_Rpb5.
DR InterPro; IPR000783; RNA_pol_subH/Rpb5_C.
DR InterPro; IPR020608; RNA_pol_subH/Rpb5_CS.
DR InterPro; IPR035913; RPB5-like_sf.
DR PANTHER; PTHR10535; PTHR10535; 1.
DR Pfam; PF01191; RNA_pol_Rpb5_C; 1.
DR SUPFAM; SSF55287; SSF55287; 1.
DR PROSITE; PS01110; RNA_POL_H_23KD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..84
FT /note="DNA-directed RNA polymerase subunit Rpo5"
FT /id="PRO_0000146103"
FT STRAND 14..17
FT /evidence="ECO:0007829|PDB:7OQ4"
FT STRAND 19..22
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 25..35
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 39..41
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 44..46
FT /evidence="ECO:0007829|PDB:7OQY"
FT HELIX 50..55
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 62..69
FT /evidence="ECO:0007829|PDB:7OQY"
FT TURN 70..72
FT /evidence="ECO:0007829|PDB:7OQY"
FT STRAND 73..82
FT /evidence="ECO:0007829|PDB:7OQY"
SQ SEQUENCE 84 AA; 9460 MW; 4B03C8B6FBA9FD9A CRC64;
MRSSSKKKID ISNHELVPKH EILQLEEAYK LVKELGIKPE QLPWIRASDP VAKSIGAKPG
DIIKITRKSP FTGESVTYRY VITG
