RPO5_THEKO
ID RPO5_THEKO Reviewed; 82 AA.
AC Q5JE31;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=DNA-directed RNA polymerase subunit Rpo5 {ECO:0000255|HAMAP-Rule:MF_00025};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00025};
DE AltName: Full=DNA-directed RNA polymerase subunit H {ECO:0000255|HAMAP-Rule:MF_00025};
GN Name=rpo5 {ECO:0000255|HAMAP-Rule:MF_00025};
GN Synonyms=rpoH {ECO:0000255|HAMAP-Rule:MF_00025}; OrderedLocusNames=TK1084;
OS Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS (Pyrococcus kodakaraensis (strain KOD1)).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=69014;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=15710748; DOI=10.1101/gr.3003105;
RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL Genome Res. 15:352-363(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase (RNAP) catalyzes the
CC transcription of DNA into RNA using the four ribonucleoside
CC triphosphates as substrates. {ECO:0000255|HAMAP-Rule:MF_00025}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00025};
CC -!- SUBUNIT: Part of the RNA polymerase complex. {ECO:0000255|HAMAP-
CC Rule:MF_00025}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00025}.
CC -!- SIMILARITY: Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase
CC subunit family. {ECO:0000255|HAMAP-Rule:MF_00025}.
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DR EMBL; AP006878; BAD85273.1; -; Genomic_DNA.
DR RefSeq; WP_011250035.1; NC_006624.1.
DR PDB; 4QIW; X-ray; 3.50 A; H/S=1-82.
DR PDB; 6KF3; EM; 3.90 A; H=1-82.
DR PDB; 6KF4; EM; 3.97 A; H=1-82.
DR PDB; 6KF9; EM; 3.79 A; H=1-82.
DR PDBsum; 4QIW; -.
DR PDBsum; 6KF3; -.
DR PDBsum; 6KF4; -.
DR PDBsum; 6KF9; -.
DR AlphaFoldDB; Q5JE31; -.
DR SMR; Q5JE31; -.
DR STRING; 69014.TK1084; -.
DR EnsemblBacteria; BAD85273; BAD85273; TK1084.
DR GeneID; 3235349; -.
DR KEGG; tko:TK1084; -.
DR PATRIC; fig|69014.16.peg.1060; -.
DR eggNOG; arCOG04258; Archaea.
DR HOGENOM; CLU_058320_4_0_2; -.
DR InParanoid; Q5JE31; -.
DR OMA; PKIYHDD; -.
DR OrthoDB; 123463at2157; -.
DR PhylomeDB; Q5JE31; -.
DR Proteomes; UP000000536; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.940.20; -; 1.
DR HAMAP; MF_00025; RNApol_Rpo5_RPB5; 1.
DR InterPro; IPR014381; Arch_Rpo5/euc_Rpb5.
DR InterPro; IPR000783; RNA_pol_subH/Rpb5_C.
DR InterPro; IPR020608; RNA_pol_subH/Rpb5_CS.
DR InterPro; IPR035913; RPB5-like_sf.
DR PANTHER; PTHR10535; PTHR10535; 1.
DR Pfam; PF01191; RNA_pol_Rpb5_C; 1.
DR SUPFAM; SSF55287; SSF55287; 1.
DR PROSITE; PS01110; RNA_POL_H_23KD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-directed RNA polymerase;
KW Nucleotidyltransferase; Reference proteome; Transcription; Transferase.
FT CHAIN 1..82
FT /note="DNA-directed RNA polymerase subunit Rpo5"
FT /id="PRO_0000146102"
FT STRAND 17..19
FT /evidence="ECO:0007829|PDB:4QIW"
FT HELIX 23..32
FT /evidence="ECO:0007829|PDB:4QIW"
FT HELIX 37..39
FT /evidence="ECO:0007829|PDB:4QIW"
FT HELIX 48..53
FT /evidence="ECO:0007829|PDB:4QIW"
FT STRAND 60..67
FT /evidence="ECO:0007829|PDB:4QIW"
FT TURN 68..70
FT /evidence="ECO:0007829|PDB:4QIW"
FT STRAND 71..80
FT /evidence="ECO:0007829|PDB:4QIW"
SQ SEQUENCE 82 AA; 9506 MW; 3479230DD5905714 CRC64;
MAAKKEFNIF DHVLVPEHRI LSEEEKEELL KKYRIRISQL PQIKASDPAV VALGAKPGDV
IEIKRKSPTA GYYYYYRLVV ED