RPOA_BACSU
ID RPOA_BACSU Reviewed; 314 AA.
AC P20429;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000255|HAMAP-Rule:MF_00059};
DE Short=RNAP subunit alpha {ECO:0000255|HAMAP-Rule:MF_00059};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_00059, ECO:0000269|PubMed:18289874};
DE AltName: Full=RNA polymerase subunit alpha {ECO:0000255|HAMAP-Rule:MF_00059};
DE AltName: Full=Transcriptase subunit alpha {ECO:0000255|HAMAP-Rule:MF_00059};
GN Name=rpoA {ECO:0000255|HAMAP-Rule:MF_00059}; OrderedLocusNames=BSU01430;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2496109; DOI=10.1128/jb.171.5.2553-2562.1989;
RA Boylan S.A., Suh J.-W., Thomas S.M., Price C.W.;
RT "Gene encoding the alpha core subunit of Bacillus subtilis RNA polymerase
RT is cotranscribed with the genes for initiation factor 1 and ribosomal
RT proteins B, S13, S11, and L17.";
RL J. Bacteriol. 171:2553-2562(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT alpha region.";
RL Gene 169:17-23(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-65.
RX PubMed=3093467; DOI=10.1128/jb.168.1.65-71.1986;
RA Suh J.-W., Boylan S.A., Price C.W.;
RT "Gene for the alpha subunit of Bacillus subtilis RNA polymerase maps in the
RT ribosomal protein gene cluster.";
RL J. Bacteriol. 168:65-71(1986).
RN [5]
RP SUBUNIT.
RC STRAIN=168;
RX PubMed=6802805; DOI=10.1128/jb.150.2.977-980.1982;
RA Achberger E.C., Tahara M., Whiteley H.R.;
RT "Interchangeability of delta subunits of RNA polymerase from different
RT species of the genus Bacillus.";
RL J. Bacteriol. 150:977-980(1982).
RN [6]
RP FUNCTION, AND SUBUNIT.
RC STRAIN=BS200;
RX PubMed=18289874; DOI=10.1016/j.pep.2008.01.006;
RA Yang X., Lewis P.J.;
RT "Overproduction and purification of recombinant Bacillus subtilis RNA
RT polymerase.";
RL Protein Expr. Purif. 59:86-93(2008).
RN [7]
RP SUBUNIT.
RC STRAIN=168;
RX PubMed=21710567; DOI=10.1002/pmic.201000790;
RA Delumeau O., Lecointe F., Muntel J., Guillot A., Guedon E., Monnet V.,
RA Hecker M., Becher D., Polard P., Noirot P.;
RT "The dynamic protein partnership of RNA polymerase in Bacillus subtilis.";
RL Proteomics 11:2992-3001(2011).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000269|PubMed:18289874}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_00059,
CC ECO:0000269|PubMed:6802805};
CC -!- SUBUNIT: Homodimer. RNAP is composed of a core of 2 alpha, a beta and a
CC beta' subunit. The core is associated with a delta subunit, and at
CC least one of epsilon or omega (PubMed:6802805, PubMed:18289874,
CC PubMed:21710567). When a sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription
CC (PubMed:18289874). {ECO:0000255|HAMAP-Rule:MF_00059,
CC ECO:0000269|PubMed:18289874, ECO:0000269|PubMed:21710567,
CC ECO:0000269|PubMed:6802805}.
CC -!- INTERACTION:
CC P20429; P25144: ccpA; NbExp=5; IntAct=EBI-5247865, EBI-5247535;
CC P20429; P39779: codY; NbExp=2; IntAct=EBI-5247865, EBI-7827914;
CC -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC transcription, whereas the C-terminal domain is involved in interaction
CC with transcriptional regulators and with upstream promoter elements.
CC {ECO:0000255|HAMAP-Rule:MF_00059}.
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000255|HAMAP-Rule:MF_00059}.
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DR EMBL; M26414; AAA22217.1; -; Genomic_DNA.
DR EMBL; L47971; AAB06826.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11919.1; -; Genomic_DNA.
DR EMBL; M13957; AAA22708.1; -; Genomic_DNA.
DR PIR; E32307; E32307.
DR RefSeq; NP_388024.1; NC_000964.3.
DR RefSeq; WP_003225835.1; NZ_JNCM01000029.1.
DR PDB; 1Z3E; X-ray; 1.50 A; B=245-314.
DR PDB; 3GFK; X-ray; 2.30 A; B=240-314.
DR PDB; 3IHQ; X-ray; 1.90 A; B=245-314.
DR PDB; 6WVJ; EM; 3.36 A; A/B=1-314.
DR PDB; 6WVK; EM; 3.36 A; A/B=1-314.
DR PDB; 6ZCA; EM; 4.20 A; U/V=1-314.
DR PDB; 6ZFB; EM; 3.90 A; U/V/u/v=1-314.
DR PDB; 7CKQ; EM; 4.40 A; A/B=1-314.
DR PDB; 7F75; EM; 4.20 A; A/B/I=1-314.
DR PDBsum; 1Z3E; -.
DR PDBsum; 3GFK; -.
DR PDBsum; 3IHQ; -.
DR PDBsum; 6WVJ; -.
DR PDBsum; 6WVK; -.
DR PDBsum; 6ZCA; -.
DR PDBsum; 6ZFB; -.
DR PDBsum; 7CKQ; -.
DR PDBsum; 7F75; -.
DR AlphaFoldDB; P20429; -.
DR SMR; P20429; -.
DR IntAct; P20429; 4.
DR MINT; P20429; -.
DR STRING; 224308.BSU01430; -.
DR jPOST; P20429; -.
DR PaxDb; P20429; -.
DR PRIDE; P20429; -.
DR EnsemblBacteria; CAB11919; CAB11919; BSU_01430.
DR GeneID; 64301981; -.
DR GeneID; 938921; -.
DR KEGG; bsu:BSU01430; -.
DR PATRIC; fig|224308.179.peg.147; -.
DR eggNOG; COG0202; Bacteria.
DR InParanoid; P20429; -.
DR OMA; LMKFRNF; -.
DR PhylomeDB; P20429; -.
DR BioCyc; BSUB:BSU01430-MON; -.
DR EvolutionaryTrace; P20429; -.
DR PRO; PR:P20429; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 2.170.120.12; -; 1.
DR Gene3D; 3.30.1360.10; -; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR PANTHER; PTHR32108; PTHR32108; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF55257; SSF55257; 1.
DR SUPFAM; SSF56553; SSF56553; 1.
DR TIGRFAMs; TIGR02027; rpoA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-directed RNA polymerase; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase.
FT CHAIN 1..314
FT /note="DNA-directed RNA polymerase subunit alpha"
FT /id="PRO_0000175265"
FT REGION 1..228
FT /note="Alpha N-terminal domain (alpha-NTD)"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00059"
FT REGION 245..314
FT /note="Alpha C-terminal domain (alpha-CTD)"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00059"
FT STRAND 8..14
FT /evidence="ECO:0007829|PDB:6WVJ"
FT HELIX 16..18
FT /evidence="ECO:0007829|PDB:6WVK"
FT STRAND 20..28
FT /evidence="ECO:0007829|PDB:6WVJ"
FT HELIX 32..44
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 49..58
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 62..65
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 71..74
FT /evidence="ECO:0007829|PDB:6WVK"
FT HELIX 75..82
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 87..93
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 95..108
FT /evidence="ECO:0007829|PDB:6WVJ"
FT HELIX 109..111
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 118..121
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 126..129
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 135..149
FT /evidence="ECO:0007829|PDB:6WVJ"
FT HELIX 151..154
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 173..185
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 188..200
FT /evidence="ECO:0007829|PDB:6WVJ"
FT STRAND 202..204
FT /evidence="ECO:0007829|PDB:6WVJ"
FT HELIX 206..225
FT /evidence="ECO:0007829|PDB:6WVJ"
FT HELIX 246..249
FT /evidence="ECO:0007829|PDB:1Z3E"
FT HELIX 253..255
FT /evidence="ECO:0007829|PDB:1Z3E"
FT HELIX 260..268
FT /evidence="ECO:0007829|PDB:1Z3E"
FT HELIX 274..278
FT /evidence="ECO:0007829|PDB:1Z3E"
FT HELIX 282..286
FT /evidence="ECO:0007829|PDB:1Z3E"
FT HELIX 293..305
FT /evidence="ECO:0007829|PDB:1Z3E"
SQ SEQUENCE 314 AA; 34799 MW; 9ADCAD891C0BCD67 CRC64;
MIEIEKPKIE TVEISDDAKF GKFVVEPLER GYGTTLGNSL RRILLSSLPG AAVTSIQIDG
VLHEFSTIEG VVEDVTTIIL HIKKLALKIY SDEEKTLEID VQGEGTVTAA DITHDSDVEI
LNPDLHIATL GENASFRVRL TAQRGRGYTP ADANKRDDQP IGVIPIDSIY TPVSRVSYQV
ENTRVGQVAN YDKLTLDVWT DGSTGPKEAI ALGSKILTEH LNIFVGLTDE AQHAEIMVEK
EEDQKEKVLE MTIEELDLSV RSYNCLKRAG INTVQELANK TEEDMMKVRN LGRKSLEEVK
AKLEELGLGL RKDD
