ATPN_YEAST
ID ATPN_YEAST Reviewed; 115 AA.
AC Q12233; D6W430;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=ATP synthase subunit g, mitochondrial;
DE Short=ATPase subunit g;
GN Name=ATP20; OrderedLocusNames=YPR020W; ORFNames=YP9531.14;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION, AND PROTEIN SEQUENCE OF 1-13.
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=9857174; DOI=10.1093/emboj/17.24.7170;
RA Arnold I., Pfeiffer K., Neupert W., Stuart R.A., Schaegger H.;
RT "Yeast mitochondrial F1F0-ATPase exists as a dimer: identification of three
RT dimer-specific subunits.";
RL EMBO J. 17:7170-7178(1998).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, PHOSPHORYLATION [LARGE SCALE
RP ANALYSIS] AT SER-3 AND SER-62, AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=17761666; DOI=10.1074/mcp.m700098-mcp200;
RA Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B.,
RA van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.;
RT "Profiling phosphoproteins of yeast mitochondria reveals a role of
RT phosphorylation in assembly of the ATP synthase.";
RL Mol. Cell. Proteomics 6:1896-1906(2007).
CC -!- FUNCTION: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or
CC Complex V) produces ATP from ADP in the presence of a proton gradient
CC across the membrane which is generated by electron transport complexes
CC of the respiratory chain. F-type ATPases consist of two structural
CC domains, F(1) - containing the extramembraneous catalytic core, and
CC F(0) - containing the membrane proton channel, linked together by a
CC central stalk and a peripheral stalk. During catalysis, ATP synthesis
CC in the catalytic domain of F(1) is coupled via a rotary mechanism of
CC the central stalk subunits to proton translocation. Part of the complex
CC F(0) domain. Minor subunit located with subunit a in the membrane.
CC -!- SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core
CC - and CF(0) - the membrane proton channel. In yeast, the dimeric form
CC of ATP synthase consists of 17 polypeptides: alpha, beta, gamma, delta,
CC epsilon, 4 (B), 5 (OSCP), 6 (A), 8, 9 (C), d, E (Tim11), f, g, h, i/j
CC and k.
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}.
CC -!- PTM: Phosphorylation on Ser-62 impairs ATP synthase dimerization.
CC -!- SIMILARITY: Belongs to the ATPase g subunit family. {ECO:0000305}.
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DR EMBL; Z71255; CAA95016.1; -; Genomic_DNA.
DR EMBL; Z49919; CAA90165.1; -; Genomic_DNA.
DR EMBL; AY692980; AAT92999.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11446.1; -; Genomic_DNA.
DR PIR; S57554; S57554.
DR RefSeq; NP_015345.1; NM_001184117.1.
DR AlphaFoldDB; Q12233; -.
DR SMR; Q12233; -.
DR BioGRID; 36197; 130.
DR ComplexPortal; CPX-3281; Mitochondrial proton-transporting ATP synthase complex.
DR DIP; DIP-1207N; -.
DR IntAct; Q12233; 3.
DR MINT; Q12233; -.
DR STRING; 4932.YPR020W; -.
DR iPTMnet; Q12233; -.
DR MaxQB; Q12233; -.
DR PaxDb; Q12233; -.
DR PRIDE; Q12233; -.
DR TopDownProteomics; Q12233; -.
DR EnsemblFungi; YPR020W_mRNA; YPR020W; YPR020W.
DR GeneID; 856131; -.
DR KEGG; sce:YPR020W; -.
DR SGD; S000006224; ATP20.
DR VEuPathDB; FungiDB:YPR020W; -.
DR eggNOG; KOG4103; Eukaryota.
DR GeneTree; ENSGT00390000009724; -.
DR HOGENOM; CLU_083674_1_0_1; -.
DR InParanoid; Q12233; -.
DR OMA; NIWTAKT; -.
DR BioCyc; YEAST:G3O-34180-MON; -.
DR Reactome; R-SCE-163210; Formation of ATP by chemiosmotic coupling.
DR Reactome; R-SCE-8949613; Cristae formation.
DR PRO; PR:Q12233; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12233; protein.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:ComplexPortal.
DR GO; GO:0005753; C:mitochondrial proton-transporting ATP synthase complex; IC:ComplexPortal.
DR GO; GO:0000276; C:mitochondrial proton-transporting ATP synthase complex, coupling factor F(o); IMP:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0015078; F:proton transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042407; P:cristae formation; IMP:SGD.
DR GO; GO:0065003; P:protein-containing complex assembly; IMP:SGD.
DR GO; GO:0015986; P:proton motive force-driven ATP synthesis; IDA:SGD.
DR InterPro; IPR006808; ATP_synth_F0_gsu_mt.
DR PANTHER; PTHR12386; PTHR12386; 1.
DR Pfam; PF04718; ATP-synt_G; 1.
PE 1: Evidence at protein level;
KW Acetylation; ATP synthesis; CF(0); Direct protein sequencing;
KW Hydrogen ion transport; Ion transport; Membrane; Mitochondrion;
KW Phosphoprotein; Reference proteome; Transport.
FT CHAIN 1..115
FT /note="ATP synthase subunit g, mitochondrial"
FT /id="PRO_0000071696"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:17761666"
FT MOD_RES 3
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17761666"
FT MOD_RES 62
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17761666"
FT MUTAGEN 62
FT /note="S->A: No effect on ATP synthase dimerization."
FT MUTAGEN 62
FT /note="S->E: Inhibits ATP synthase dimerization."
SQ SEQUENCE 115 AA; 12921 MW; 20A8F381319B9697 CRC64;
MLSRIQNYTS GLVSKANLLS SKALYYGKVG AEISKQIYLK EGLQPPTVAQ FKSVYSNLYK
QSLNFALKPT EVLSCLKNIQ KNELLKYGAY GIQLIGFYSV GEIIGRRKLV GYKHH
