ID   RPOA_EUGLO              Reviewed;         220 AA.
AC   P34778; P58144;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-2002, sequence version 2.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha;
DE            Short=PEP;
DE            EC=2.7.7.6;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit alpha;
DE            Short=RNA polymerase subunit alpha;
GN   Name=rpoA;
OS   Euglena longa (Euglenophycean alga) (Astasia longa).
OG   Plastid; Non-photosynthetic plastid.
OC   Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC   Euglenales; Euglenaceae; Euglena.
OX   NCBI_TaxID=3037;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CCAP 1204-17a;
RX   PubMed=7859309; DOI=10.1007/bf00309557;
RA   Gockel G., Hachtel W., Baier S., Fliss C., Henke M.;
RT   "Genes for components of the chloroplast translational apparatus are
RT   conserved in the reduced 73-kb plastid DNA of the nonphotosynthetic
RT   euglenoid flagellate Astasia longa.";
RL   Curr. Genet. 26:256-262(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCAP 1204-17a;
RX   PubMed=11212895; DOI=10.1078/s1434-4610(04)70033-4;
RA   Gockel G., Hachtel W.;
RT   "Complete gene map of the plastid genome of the nonphotosynthetic euglenoid
RT   flagellate Astasia longa.";
RL   Protist 151:347-351(2000).
RN   [3]
RP   IDENTIFICATION OF GENE.
RX   PubMed=11861918; DOI=10.1093/nar/30.5.1247;
RA   Sheveleva E.V., Giordani N.V., Hallick R.B.;
RT   "Identification and comparative analysis of the chloroplast alpha-subunit
RT   gene of DNA-dependent RNA polymerase from seven Euglena species.";
RL   Nucleic Acids Res. 30:1247-1254(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid.
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The C-terminal domain thought to be required for interaction
CC       with some regulatory factors is missing from this protein.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC24588.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAC24589.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AJ294725; CAC24589.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AJ294725; CAC24588.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S38600; S38600.
DR   RefSeq; NP_074977.1; NC_002652.1.
DR   RefSeq; NP_074978.1; NC_002652.1.
DR   AlphaFoldDB; P34778; -.
DR   SMR; P34778; -.
DR   GeneID; 1457310; -.
DR   GeneID; 802530; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.170.120.12; -; 1.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..220
FT                   /note="DNA-directed RNA polymerase subunit alpha"
FT                   /id="PRO_0000175504"
SQ   SEQUENCE   220 AA;  26287 MW;  D221F70D995BB5AB CRC64;
     MKYIKIYILR NKKFKNQNIN FLKIKPFNCP NYNFFGNEIR RSLLLNIKKT MTITNIKFFI
     SKSKSKHPKK LKIFHPVNEF FDIEEIKENL SNICINLKKL RFKFNPKNNK EKKTFIILNS
     FNKKSFSAKD IIIPSTSNLK IVNLKEHLFN KISQKINLKV LIKIENNENY FYNPIKRINF
     ILEKNNNIGK YIILDLNSDI NINPFKAFIE SLKYLNLNNT