ID   RPOA_HELSJ              Reviewed;         246 AA.
AC   Q2EEW7;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha;
DE            Short=PEP;
DE            EC=2.7.7.6;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit alpha;
DE            Short=RNA polymerase subunit alpha;
GN   Name=rpoA;
OS   Helicosporidium sp. subsp. Simulium jonesii (Green alga).
OG   Plastid; Non-photosynthetic plastid.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC   Chlorellales; Chlorellaceae; Helicosporidium; unclassified Helicosporidium.
OX   NCBI_TaxID=145475;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16630350; DOI=10.1186/1741-7007-4-12;
RA   de Koning A.P., Keeling P.J.;
RT   "The complete plastid genome sequence of the parasitic green alga,
RT   Helicosporidium sp. is highly reduced and structured.";
RL   BMC Biol. 4:12-12(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (Potential).
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Plastid.
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC       {ECO:0000305}.
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DR   EMBL; DQ398104; ABD33975.1; -; Genomic_DNA.
DR   RefSeq; YP_635927.1; NC_008100.1.
DR   AlphaFoldDB; Q2EEW7; -.
DR   SMR; Q2EEW7; -.
DR   GeneID; 4100421; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.170.120.12; -; 1.
DR   Gene3D; 3.30.1360.10; -; 1.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..246
FT                   /note="DNA-directed RNA polymerase subunit alpha"
FT                   /id="PRO_0000296893"
SQ   SEQUENCE   246 AA;  28093 MW;  10FCD871F0D0274F CRC64;
     MLIKQLSSSY NEYNNNYAAS FLIGPLPEGI AQSLGFNIRS VLLKEFPYFA VKNIRFNPEN
     NNYKTHIGTK ESIETIINTI LGIDFNYSFD FYKAMGLYKS KPKALFQENL SKAKFLCSAK
     QVGEGILYAK DLMLPVGIKC TTPYKIIAHV TKQNFLGFTF ELGFLFGLNN NIKFENKLYS
     IKTYPIQNIN IEIINSKKSF NLNSESIILS FTTKSSLQPI DVIKILSYLL LNKHLILIKA
     FQQFLD