ATPZ_ECOLI
ID ATPZ_ECOLI Reviewed; 126 AA.
AC P0ABC0; P03808; P76747; Q2M857; Q47248;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=ATP synthase protein I;
GN Name=atpI; Synonyms=uncI; OrderedLocusNames=b3739, JW5611;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6395859; DOI=10.1042/bj2240799;
RA Walker J.E., Gay N.J., Saraste M., Eberle A.N.;
RT "DNA sequence around the Escherichia coli unc operon. Completion of the
RT sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and
RT phoS.";
RL Biochem. J. 224:799-815(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6272190; DOI=10.1093/nar/9.16.3919;
RA Gay N.J., Walker J.E.;
RT "The atp operon: nucleotide sequence of the promoter and the genes for the
RT membrane proteins, and the delta subunit of Escherichia coli ATP-
RT synthase.";
RL Nucleic Acids Res. 9:3919-3926(1981).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6206892; DOI=10.1016/0304-4173(84)90003-x;
RA Walker J.E., Saraste M., Gay N.J.;
RT "The unc operon. Nucleotide sequence, regulation and structure of ATP-
RT synthase.";
RL Biochim. Biophys. Acta 768:164-200(1984).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=6318052; DOI=10.1007/bf00327415;
RA Nielsen J., Joergensen B.B., von Meyenburg K., Hansen F.G.;
RT "The promoters of the atp operon of Escherichia coli K12.";
RL Mol. Gen. Genet. 193:64-71(1984).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6301339; DOI=10.1111/j.1749-6632.1982.tb25731.x;
RA Kanazawa H., Futai M.;
RT "Structure and function of H+-ATPase: what we have learned from Escherichia
RT coli H+-ATPase.";
RL Ann. N. Y. Acad. Sci. 402:45-64(1982).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT organizational symmetry around the origin of replication.";
RL Genomics 16:551-561(1993).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [9]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-108.
RX PubMed=6327640; DOI=10.1128/jb.158.3.820-825.1984;
RA Gay N.J.;
RT "Construction and characterization of an Escherichia coli strain with a
RT uncI mutation.";
RL J. Bacteriol. 158:820-825(1984).
RN [10]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80.
RX PubMed=6215041; DOI=10.1016/0006-291x(82)91529-7;
RA Kanazawa H., Mabuchi K., Futai M.;
RT "Nucleotide sequence of the promoter region of the gene cluster for proton-
RT translocating ATPase from Escherichia coli and identification of the active
RT promotor.";
RL Biochem. Biophys. Res. Commun. 107:568-575(1982).
RN [11]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-126.
RX PubMed=6278247; DOI=10.1007/bf00271191;
RA Nielsen J., Hansen F.G., Hoppe J., Friedl P., von Meyenburg K.;
RT "The nucleotide sequence of the atp genes coding for the F0 subunits a, b,
RT c and the F1 subunit delta of the membrane bound ATP synthase of
RT Escherichia coli.";
RL Mol. Gen. Genet. 184:33-39(1981).
RN [12]
RP PROTEIN SEQUENCE OF 2-8.
RX PubMed=2136739; DOI=10.1016/s0021-9258(19)40242-1;
RA Schneppe B., Deckers-Hebesteit G., Altendorf K.;
RT "Overproduction and purification of the uncI gene product of the ATP
RT synthase of Escherichia coli.";
RL J. Biol. Chem. 265:389-395(1990).
RN [13]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: A possible function for this protein is to guide the assembly
CC of the membrane sector of the ATPase enzyme complex.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the bacterial AtpI family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA24730.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAA62091.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAA83868.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA23513.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA25640.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAA25775.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; X01631; CAA25775.1; ALT_INIT; Genomic_DNA.
DR EMBL; X01383; CAA25640.1; ALT_INIT; Genomic_DNA.
DR EMBL; V00264; CAA23513.1; ALT_INIT; Genomic_DNA.
DR EMBL; J01594; AAA24730.1; ALT_INIT; Genomic_DNA.
DR EMBL; M25464; AAA83868.1; ALT_INIT; Genomic_DNA.
DR EMBL; L10328; AAA62091.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76762.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77549.1; -; Genomic_DNA.
DR RefSeq; NP_418195.2; NC_000913.3.
DR RefSeq; WP_000116695.1; NZ_SSZK01000036.1.
DR AlphaFoldDB; P0ABC0; -.
DR BioGRID; 4259565; 5.
DR STRING; 511145.b3739; -.
DR PaxDb; P0ABC0; -.
DR PRIDE; P0ABC0; -.
DR EnsemblBacteria; AAC76762; AAC76762; b3739.
DR EnsemblBacteria; BAE77549; BAE77549; BAE77549.
DR GeneID; 66672357; -.
DR GeneID; 948251; -.
DR KEGG; ecj:JW5611; -.
DR KEGG; eco:b3739; -.
DR PATRIC; fig|1411691.4.peg.2961; -.
DR EchoBASE; EB0104; -.
DR eggNOG; COG3312; Bacteria.
DR HOGENOM; CLU_121415_2_0_6; -.
DR OMA; YMYMQVE; -.
DR PhylomeDB; P0ABC0; -.
DR BioCyc; EcoCyc:EG10106-MON; -.
DR PRO; PR:P0ABC0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0045263; C:proton-transporting ATP synthase complex, coupling factor F(o); IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR005598; ATP_synth_I.
DR Pfam; PF03899; ATP-synt_I; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; CF(0); Direct protein sequencing;
KW Hydrogen ion transport; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2136739"
FT CHAIN 2..126
FT /note="ATP synthase protein I"
FT /id="PRO_0000071706"
FT TOPO_DOM 2..14
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..37
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..70
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..100
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..126
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 37
FT /note="G -> A (in Ref. 5; AAA83868)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 126 AA; 13632 MW; 308FC1C16059C5A6 CRC64;
MSVSLVSRNV ARKLLLVQLL VVIASGLLFS LKDPFWGVSA ISGGLAVFLP NVLFMIFAWR
HQAHTPAKGR VAWTFAFGEA FKVLAMLVLL VVALAVLKAV FLPLIVTWVL VLVVQILAPA
VINNKG