ID   RPOA_RECAM              Reviewed;         222 AA.
AC   O21260;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha, mitochondrial;
DE            Short=RNA polymerase subunit alpha;
DE            EC=2.7.7.6;
DE   AltName: Full=Transcriptase alpha chain;
GN   Name=rpoA;
OS   Reclinomonas americana.
OG   Mitochondrion.
OC   Eukaryota; Discoba; Jakobida; Histionina; Histionidae; Reclinomonas.
OX   NCBI_TaxID=48483;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 50394;
RX   PubMed=9168110; DOI=10.1038/387493a0;
RA   Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C.,
RA   Sankoff D., Turmel M., Gray M.W.;
RT   "An ancestral mitochondrial DNA resembling a eubacterial genome in
RT   miniature.";
RL   Nature 387:493-497(1997).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC       {ECO:0000305}.
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DR   EMBL; AF007261; AAD11887.1; -; Genomic_DNA.
DR   PIR; S78154; S78154.
DR   RefSeq; NP_044772.1; NC_001823.1.
DR   AlphaFoldDB; O21260; -.
DR   SMR; O21260; -.
DR   GeneID; 801064; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.170.120.12; -; 1.
DR   Gene3D; 3.30.1360.10; -; 1.
DR   InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR   InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR   InterPro; IPR011773; DNA-dir_RpoA.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   PANTHER; PTHR32108; PTHR32108; 1.
DR   Pfam; PF01000; RNA_pol_A_bac; 1.
DR   Pfam; PF01193; RNA_pol_L; 1.
DR   SMART; SM00662; RPOLD; 1.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Mitochondrion; Nucleotidyltransferase;
KW   Transcription; Transferase.
FT   CHAIN           1..222
FT                   /note="DNA-directed RNA polymerase subunit alpha,
FT                   mitochondrial"
FT                   /id="PRO_0000175511"
SQ   SEQUENCE   222 AA;  25376 MW;  1D48F788696CB79A CRC64;
     MVHKTPSFRP IIHVLKHDFN SIQVLIGPLE KNFSGSFANL LRKIIFRYTS GVSIIGLKIN
     GIDSLHSYSL IPGVKEDVLN VLNNLKHLLI QIDDYDYKPI LLTIQKKGPY IIKSKDIICP
     SNIKILNDDL YICTLENDSQ IDLTLIGDIG CGYVLSTYFG NRKSIVYSDT NFCPIKHVSY
     YVRSYTTYEE LIFYIKTNGT CNPLFVMKNA FSFLKDKINI TF