RPOA_RHIME
ID RPOA_RHIME Reviewed; 336 AA.
AC Q925Z2;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=DNA-directed RNA polymerase subunit alpha;
DE Short=RNAP subunit alpha;
DE EC=2.7.7.6;
DE AltName: Full=RNA polymerase subunit alpha;
DE AltName: Full=Transcriptase subunit alpha;
GN Name=rpoA; OrderedLocusNames=R01380; ORFNames=SMc01285;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION IN E.COLI.
RC STRAIN=1021;
RX PubMed=12081950; DOI=10.1128/jb.184.14.3808-3814.2002;
RA Peck M.C., Gaal T., Fisher R.F., Gourse R.L., Long S.R.;
RT "The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble
RT with RNA polymerase subunits from Escherichia coli and function in basal
RT and activated transcription both in vivo and in vitro.";
RL J. Bacteriol. 184:3808-3814(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6;
CC -!- SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1
CC beta, 1 beta' and 1 omega subunit. When a sigma factor is associated
CC with the core the holoenzyme is formed, which can initiate
CC transcription (By similarity). {ECO:0000250}.
CC -!- DOMAIN: The alpha-NTD is essential for RNAP assembly and basal
CC transcription, whereas the alpha-CTD is involved in interaction with
CC transcriptional regulators and with upstream promoter elements (By
CC similarity). The alpha-NTD recognizes upstream AT-rich elements and the
CC alpha-CTD interacts with the transcriptional activator Fis in
CC reconstitution assays in E.coli. {ECO:0000250}.
CC -!- MISCELLANEOUS: Able to replace the endogenous E.coli protein.
CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC {ECO:0000305}.
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DR EMBL; AF317474; AAL26901.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC45959.1; -; Genomic_DNA.
DR RefSeq; NP_385486.1; NC_003047.1.
DR RefSeq; WP_003536494.1; NC_003047.1.
DR AlphaFoldDB; Q925Z2; -.
DR SMR; Q925Z2; -.
DR STRING; 266834.SMc01285; -.
DR EnsemblBacteria; CAC45959; CAC45959; SMc01285.
DR GeneID; 61614906; -.
DR KEGG; sme:SMc01285; -.
DR PATRIC; fig|266834.11.peg.2797; -.
DR eggNOG; COG0202; Bacteria.
DR HOGENOM; CLU_053084_0_0_5; -.
DR OMA; LMKFRNF; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 2.170.120.12; -; 1.
DR Gene3D; 3.30.1360.10; -; 1.
DR HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR InterPro; IPR011773; DNA-dir_RpoA.
DR InterPro; IPR036603; RBP11-like.
DR InterPro; IPR011260; RNAP_asu_C.
DR InterPro; IPR036643; RNApol_insert_sf.
DR PANTHER; PTHR32108; PTHR32108; 1.
DR Pfam; PF01000; RNA_pol_A_bac; 1.
DR Pfam; PF03118; RNA_pol_A_CTD; 1.
DR Pfam; PF01193; RNA_pol_L; 1.
DR SMART; SM00662; RPOLD; 1.
DR SUPFAM; SSF55257; SSF55257; 1.
DR SUPFAM; SSF56553; SSF56553; 1.
DR TIGRFAMs; TIGR02027; rpoA; 1.
PE 1: Evidence at protein level;
KW DNA-directed RNA polymerase; Nucleotidyltransferase; Reference proteome;
KW Transcription; Transferase.
FT CHAIN 1..336
FT /note="DNA-directed RNA polymerase subunit alpha"
FT /id="PRO_0000175365"
FT REGION 1..232
FT /note="Alpha N-terminal domain (alpha-NTD)"
FT /evidence="ECO:0000250"
FT REGION 248..336
FT /note="Alpha C-terminal domain (alpha-CTD)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 336 AA; 37171 MW; 70C7CCBBA10CA81D CRC64;
MIQKNWQELI KPNKVEFASS GRTKATLVAE PLERGFGLTL GNALRRVLLS SLRGAAVTAV
QIDGVLHEFS SIPGVREDVT DIVLNIKEIA IKMDGDDAKR MVVRKQGPGV VTAGDIQTVG
DIEILNPNHV ICTLDEGAEI RMEFTVNNGK GYVPADRNRS EDAPIGLIPV DSLYSPVKKV
SYKVENTREG QVLDYDKLTM SIETDGSVTG EDAIAFAARI LQDQLSVFVN FDEPQKETEE
EAVTELAFNP ALLKKVDELE LSVRSANCLK NDNIVYIGDL IQKTEAEMLR TPNFGRKSLN
EIKEVLASMG LHLGMEVPSW PPENIEDLAK RYEDQY
