ID   RPOA_SINAL              Reviewed;         327 AA.
AC   Q9XQN8;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha;
DE            Short=PEP;
DE            EC=2.7.7.6;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit alpha;
DE            Short=RNA polymerase subunit alpha;
GN   Name=rpoA;
OS   Sinapis alba (White mustard) (Brassica hirta).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Sinapis.
OX   NCBI_TaxID=3728;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=cv. Albatros; TISSUE=Cotyledon;
RX   PubMed=10601874; DOI=10.1046/j.1432-1327.2000.00991.x;
RA   Pfannschmidt T., Ogrzewalla K., Baginsky S., Sickmann A., Meyer H.E.,
RA   Link G.;
RT   "The multisubunit chloroplast RNA polymerase A from mustard (Sinapis alba
RT   L.). Integration of a prokaryotic core into a larger complex with
RT   organelle-specific functions.";
RL   Eur. J. Biochem. 267:253-261(2000).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: The minimal PEP RNA polymerase found in etioplasts (PEP-B) is
CC       composed of four subunits: alpha, beta, beta', and beta''. Following
CC       differentiation into chloroplasts the PEP-A RNA polymerase in this
CC       organism has been shown to be composed of at least 13 subunits,
CC       including the PEP-B subunits. When a (nuclear-encoded) sigma factor is
CC       associated with the core the holoenzyme is formed, which can initiate
CC       transcription (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC       transcription, whereas the C-terminal domain is involved in interaction
CC       with transcriptional regulators and with upstream promoter elements.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC       {ECO:0000305}.
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DR   EMBL; AJ243345; CAB51131.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9XQN8; -.
DR   SMR; Q9XQN8; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.170.120.12; -; 1.
DR   Gene3D; 3.30.1360.10; -; 1.
DR   HAMAP; MF_00059; RNApol_bact_RpoA; 1.
DR   InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR   InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR   InterPro; IPR011773; DNA-dir_RpoA.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR011260; RNAP_asu_C.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   PANTHER; PTHR32108; PTHR32108; 1.
DR   Pfam; PF01000; RNA_pol_A_bac; 1.
DR   Pfam; PF03118; RNA_pol_A_CTD; 1.
DR   Pfam; PF01193; RNA_pol_L; 1.
DR   SMART; SM00662; RPOLD; 1.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
DR   TIGRFAMs; TIGR02027; rpoA; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..327
FT                   /note="DNA-directed RNA polymerase subunit alpha"
FT                   /id="PRO_0000175496"
FT   REGION          1..233
FT                   /note="Alpha N-terminal domain (alpha-NTD)"
FT                   /evidence="ECO:0000250"
FT   REGION          264..327
FT                   /note="Alpha C-terminal domain (alpha-CTD)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   327 AA;  37822 MW;  826B242A43B1CE92 CRC64;
     MVREKVKVST RTLQWKCVES RRDSKRLYYG RFILSPLMKG QADTIGIAMA RALLGEIEGT
     CITRAKSENI PHDYSNIVGI QESVHEILMN LNEIVLKSNL YGTRNPFICV QGPGYITARD
     IILPPSVEIV DNTQHIATLT EPINLCIGLK IERNRGYSLK MSNNFEDRSY PIDAVFMPVQ
     NANHSIHSYG NGNEKQEILF IEIWTNGSLT PKEALYEASR NLINLFIPFL HVEEETFYLE
     NNQHQVTLPV FPFHNKLVNL RQKKKELAFQ YIFIDQLELP PRIYNCLKKS NIHTLLDLLN
     NSQEDLIKME HFHIEDVKKL LDILEKK