ID   RPOA_STIHE              Reviewed;         517 AA.
AC   Q06SF7;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha;
DE            Short=PEP;
DE            EC=2.7.7.6;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit alpha;
DE            Short=RNA polymerase subunit alpha;
GN   Name=rpoA;
OS   Stigeoclonium helveticum (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   OCC clade; Chaetophorales; Chaetophoraceae; Stigeoclonium.
OX   NCBI_TaxID=55999;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 441;
RX   PubMed=16944205; DOI=10.1007/s00438-006-0156-2;
RA   Belanger A.-S., Brouard J.-S., Charlebois P., Otis C., Lemieux C.,
RA   Turmel M.;
RT   "Distinctive architecture of the chloroplast genome in the chlorophycean
RT   green alga Stigeoclonium helveticum.";
RL   Mol. Genet. Genomics 276:464-477(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC       transcription, whereas the C-terminal domain is involved in interaction
CC       with transcriptional regulators and with upstream promoter elements.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ630521; ABF60189.1; -; Genomic_DNA.
DR   RefSeq; YP_764409.1; NC_008372.1.
DR   AlphaFoldDB; Q06SF7; -.
DR   SMR; Q06SF7; -.
DR   GeneID; 4308369; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.170.120.12; -; 1.
DR   Gene3D; 3.30.1360.10; -; 1.
DR   InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR   InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR   InterPro; IPR011773; DNA-dir_RpoA.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   PANTHER; PTHR32108; PTHR32108; 1.
DR   Pfam; PF01000; RNA_pol_A_bac; 1.
DR   Pfam; PF01193; RNA_pol_L; 1.
DR   SMART; SM00662; RPOLD; 1.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..517
FT                   /note="DNA-directed RNA polymerase subunit alpha"
FT                   /id="PRO_0000296910"
SQ   SEQUENCE   517 AA;  59323 MW;  A483F45D05084D73 CRC64;
     MGNYKSNLET KIGVVKNPIL VSCRESIMEN KKSFYGRFYI GPLEVGQGIT LANALRRALL
     SELNGLAITT VEIEGVSHEY STLMGVRESV LDILLNLKQI VLKSKKSVKR AQTAYIYCQG
     PGVIRAGDII LPSSIQCVDP EQYIATLSSD GILKMKVIIR QGKNYLVQTP NSLFFDEVSE
     FVLDKNNLAE KRSEKQSFQH KIFLQNSVNK KKWGKVEEKK EPKLSFFSHS GSRPSFLFKT
     KVGLKSKALT FSFQNSRSLY TISQNSKNKE LFINLFKKQI YSILALKVSI TKSQDPNNFS
     NLLCSFFLTS KNKTKPLFID AVFMPVTKVN YTLEENKQKL FDEIYPIVSN DNLEKNWVKV
     KKSGFFLSNQ QLSFEKNSLF EESKLQQNTN NEAKHKNNGN GKFVDSKLLK QSTTSNFDYN
     YWSLFSAELN NNSSSWTTLN SNIFFEHFNQ SPKDIIILEI WTNGSILPRT ALKHATQNLS
     NLFIKFQNAK MMKNSFFETN KTYTQTIRQL YEKYQNF