ID   RPOA_TETOB              Reviewed;         478 AA.
AC   P19587; Q1KVS4;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=DNA-directed RNA polymerase subunit alpha;
DE            Short=PEP;
DE            EC=2.7.7.6;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit alpha;
DE            Short=RNA polymerase subunit alpha;
GN   Name=rpoA;
OS   Tetradesmus obliquus (Green alga) (Acutodesmus obliquus).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus.
OX   NCBI_TaxID=3088;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=UTEX 393;
RX   PubMed=9185572; DOI=10.1093/nar/25.13.2610;
RA   Turmel M., Otis C., Cote V., Lemieux C.;
RT   "Evolutionarily conserved and functionally important residues in the I-CeuI
RT   homing endonuclease.";
RL   Nucleic Acids Res. 25:2610-2619(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 393;
RX   PubMed=16638149; DOI=10.1186/1471-2148-6-37;
RA   de Cambiaire J.-C., Otis C., Lemieux C., Turmel M.;
RT   "The complete chloroplast genome sequence of the chlorophycean green alga
RT   Scenedesmus obliquus reveals a compact gene organization and a biased
RT   distribution of genes on the two DNA strands.";
RL   BMC Evol. Biol. 6:37-37(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-129.
RC   STRAIN=KS3/2;
RX   PubMed=2476655; DOI=10.1007/bf00331276;
RA   Kueck U.;
RT   "The intron of a plastid gene from a green alga contains an open reading
RT   frame for a reverse transcriptase-like enzyme.";
RL   Mol. Gen. Genet. 218:257-265(1989).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal
CC       transcription, whereas the C-terminal domain is involved in interaction
CC       with transcriptional regulators and with upstream promoter elements.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family.
CC       {ECO:0000305}.
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DR   EMBL; DQ396875; ABD48283.1; -; Genomic_DNA.
DR   RefSeq; YP_636000.1; NC_008101.1.
DR   AlphaFoldDB; P19587; -.
DR   SMR; P19587; -.
DR   PRIDE; P19587; -.
DR   GeneID; 4099772; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.170.120.12; -; 1.
DR   Gene3D; 3.30.1360.10; -; 1.
DR   InterPro; IPR011262; DNA-dir_RNA_pol_insert.
DR   InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3.
DR   InterPro; IPR011773; DNA-dir_RpoA.
DR   InterPro; IPR036603; RBP11-like.
DR   InterPro; IPR036643; RNApol_insert_sf.
DR   PANTHER; PTHR32108; PTHR32108; 1.
DR   Pfam; PF01000; RNA_pol_A_bac; 1.
DR   Pfam; PF01193; RNA_pol_L; 1.
DR   SMART; SM00662; RPOLD; 1.
DR   SUPFAM; SSF55257; SSF55257; 1.
DR   SUPFAM; SSF56553; SSF56553; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..478
FT                   /note="DNA-directed RNA polymerase subunit alpha"
FT                   /id="PRO_0000175493"
FT   REGION          1..341
FT                   /note="Alpha N-terminal domain (alpha-NTD)"
FT                   /evidence="ECO:0000250"
FT   REGION          365..478
FT                   /note="Alpha C-terminal domain (alpha-CTD)"
FT                   /evidence="ECO:0000250"
FT   VARIANT         3
FT                   /note="K -> E (in strain: KS3/2)"
SQ   SEQUENCE   478 AA;  55545 MW;  5F0C4C8D1E64838B CRC64;
     MNKKIQDFFL SCKECILENP RSFYGSFSLG PFKNSQSLTV ANALRRTLLA ELSNIAITHL
     EIEGVTHEYS TLVGVRESVL DLLLNFKGIA LKNTSPVTKP LFGYLQVRGP GVVRASDLKF
     PPMIQCVDPD QYIATLNENG KLILKFRISD FKNSQENLNF EVSKTFLNIP NFGHFDTSSF
     QNFENSNFFS SSFFASQKMI KSKRKTKFSQ LKVDYLSSFG FSTQHKRSGL NQTQKFAMQH
     QNPFRIEKNQ QDFNKNKNGN FDSQKNKTNS LWVDPLFNPI LKVNYIIETI EPMQKNIPNE
     IVFIELWTNG SIHPRKAFYT ALLYLKTMFD KLDCMRLLNY EFSNTMLESE KTSTKFFKTF
     EYDFRFYNSR EDKTMKSFTP EKFFLPEEIE DVEKDYLLNL KNQADNTWND LPLTNLNLPY
     RITKILAKNN FFVVGDLLKI SPNELKKLSG IGNYCVFILQ KRFEKLGLKL GSRNEKNL