ID   RPOB1_CHLRE             Reviewed;         822 AA.
AC   Q8HTL6; B7U1I2;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta N-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta N-terminal section;
DE            Short=RNA polymerase subunit beta N-terminal section;
GN   Name=rpoB1;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1617738; DOI=10.1007/bf00351659;
RA   Fong S.E., Surzycki S.J.;
RT   "Chloroplast RNA polymerase genes of Chlamydomonas reinhardtii exhibit an
RT   unusual structure and arrangement.";
RL   Curr. Genet. 21:485-497(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA   Smith D.R., Lee R.W.;
RT   "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT   addressing the mutational-hazard hypothesis.";
RL   BMC Evol. Biol. 9:120-120(2009).
RN   [3]
RP   IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX   PubMed=12417694; DOI=10.1105/tpc.006155;
RA   Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA   Stern D.B.;
RT   "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT   sea of repeats.";
RL   Plant Cell 14:2659-2679(2002).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In C.reinhardtii the gene for this protein is split in
CC       two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; AY160684; AAN41266.1; -; Genomic_DNA.
DR   EMBL; FJ423446; ACJ50129.1; -; Genomic_DNA.
DR   EMBL; BK000554; DAA00943.1; -; Genomic_DNA.
DR   PIR; S26872; S26872.
DR   RefSeq; NP_958398.1; NC_005353.1.
DR   AlphaFoldDB; Q8HTL6; -.
DR   SMR; Q8HTL6; -.
DR   STRING; 3055.DAA00943; -.
DR   PaxDb; Q8HTL6; -.
DR   PRIDE; Q8HTL6; -.
DR   GeneID; 2716993; -.
DR   KEGG; cre:ChreCp042; -.
DR   eggNOG; KOG0214; Eukaryota.
DR   HOGENOM; CLU_000524_4_0_1; -.
DR   InParanoid; Q8HTL6; -.
DR   OrthoDB; 944344at2759; -.
DR   Proteomes; UP000006906; Chloroplast.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Reference proteome; Transcription; Transferase.
FT   CHAIN           1..822
FT                   /note="DNA-directed RNA polymerase subunit beta N-terminal
FT                   section"
FT                   /id="PRO_0000048016"
FT   REGION          376..408
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        379..408
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   822 AA;  93185 MW;  3B3FE77E72E9305E CRC64;
     MLNISTNMMI NQTLNFDSNQ ESQKNKTERI SKTKLTRELI TKTDHDIYVD DISFLQREDN
     VNVSQIDLTA RGVLNPEDQG VLHKSTEFGV FNKNKTGLPA SVDLKIKQNS FFSVKQPDFN
     KYLISDFVEI QRNSFFTLLE KGIIEEFSKR NPITNSKKTM EIFFYPDYYQ LTPPEYSPSQ
     AIIKSKSYTS KLYIPVQLTD KSKNIIKLKW VYIGDIPLMT KRGHFILNGC ARVIVNQMVR
     SPGIYYQKKI YENFSNKWSE KPENTFTRYF ADLICNRGTW LRIEMDKYNK IWAQMKRVPK
     IPIMWFLIAV GLTDKIVLKT VMDSKILLYN LDEDPLNPRK KPLPYVKTPP AAWSAIYNIV
     FAKKIKAQEK AKKMLELTEG NPSSKSQTKN KTSASKKSKT LNVANTKGKT PAENIKTLSE
     LIDLEKALFL KSEQGRKWIF NKFMNPRTYD LGKVGRVNFN RKLKLSISQD ITTLTPQDLL
     AATNNLILVS KGLRELDDID HLKNRRVRTS GELIQIQIGV GLVRLEKTIR EKMTYASGLS
     SLPSQKFAFR SSKQRNQSPV GDAENATQLT IGNLINTKPF NGALREFFGT SPLSQFMDQI
     NPLAELTHKR RLSSMGPGGV TRDSATLAIR GIHPSHYGRI CPVETPEGKN TGLVNSLTAY
     ARVNAAGYIE TPFYRVYKGQ VQKKTGLYFF SAKQEEKIKL GAPDLYTSEI GFLPKASIPV
     RIVEDFTKIS RNEIQYVGVA PIQMISIATS LIPFFEHDDA NRALMGSNMQ RQAVPILKPQ
     RPIVGTGLEA RAVSDSGHVI TAKSSGIVMY TSSKEIIIYS LQ