ID   RPOB1_PLETE             Reviewed;        1142 AA.
AC   A6YGD9;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta N-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta N-terminal section;
DE            Short=RNA polymerase subunit beta N-terminal section;
GN   Name=rpoB1; Synonyms=rpoBa;
OS   Pleurastrum terricola (Filamentous green alga) (Leptosira terrestris).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Pleurastraceae; Pleurastrum.
OX   NCBI_TaxID=34116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCAP 463/2 / UTEX 333;
RX   PubMed=17610731; DOI=10.1186/1471-2164-8-213;
RA   de Cambiaire J.-C., Otis C., Turmel M., Lemieux C.;
RT   "The chloroplast genome sequence of the green alga Leptosira terrestris:
RT   multiple losses of the inverted repeat and extensive genome rearrangements
RT   within the Trebouxiophyceae.";
RL   BMC Genomics 8:213-213(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In L.terrestris the gene for this protein is split in
CC       two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; EF506945; ABO69351.1; -; Genomic_DNA.
DR   RefSeq; YP_001382216.1; NC_009681.1.
DR   AlphaFoldDB; A6YGD9; -.
DR   PRIDE; A6YGD9; -.
DR   GeneID; 5383722; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 2.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..1142
FT                   /note="DNA-directed RNA polymerase subunit beta N-terminal
FT                   section"
FT                   /id="PRO_0000308257"
SQ   SEQUENCE   1142 AA;  131864 MW;  D734F56BD1A3B612 CRC64;
     MEFSVKNKNL LIQNLVEIQQ QSFNELLCVG LKRQLLQLNF IENKTRNIKL IFHAKNYKFI
     LPEITPKEAV LKSKTFASAL FIPVEYRAKN SIGLYWVLLG HLPFMTKRGH FIINGVPRVV
     LNQMVRSPGL YYKTTANPQN VSNQVSINDS SHTSPVFYSD IIPNRGTWLR LEIDRKKNIW
     IRMKRVDKIP MMLFLQALGY DLHCIEKLLY SRKFLNCDYL LNTDHPADSN SALKKIKVEI
     EQQKVLKLAA LEALDDDDPD IAKQLDYIEK TTTPEMGKDF LFQSFFNPAS YDLSPIGRFQ
     LNKKLNLSIP SDYTVLTEFD IYFATKELIK RATTMSNSDD IDHLQTRRIR SCGELLEIQL
     GEGIERLQKT IIDKINNTNL KKFAKHSSTY KRGTSSSIYR QDRQVINTDK NSKFLKNNLP
     TKFNRITLEP NLLPNGTSFI HKNNKLNQVS SNKLKLLSKK LIYLNLLINQ KFIIAKLKSA
     NLLANKSSFD KFLIHLVDES PRQKFVKNKT GLYHKFIPTP EIYIVEPNLN LVDMKSQQKF
     NLLKMDLYKV GLAESFLKSN LNNLNIYLQM CLRKPNTYFR DISKICSAQI TQQLKQKSFL
     KLLLINPRLI SQNNLLVLNR AVNFLKKIQF NLKDSPNLND YQNFEINKLK AHIFKCQIKT
     QNNLTCCKYI YIKTKFNYIN LPIKQFFFTK RFLITEQKLL LGGKFINSNL ALTSLLKLHK
     FSEVVSNSFL IYNANNPNPN PTSIMSMIRP VNKSEIYRQL NRRFVRSRLQ LNVLKSELTN
     SHSQLIKANN DPIDRELSIQ ILKQDVQKSN PFNKKGIYNQ RFINKKFLDT SQFIDQELLH
     FLKTHKIFLD RQLTSLKTKQ QNEKLKFIKW KKLRNFKNAL KNPIQNLITT NAINGALQEL
     FGLNPLSQFM DQINPLSEIT HKRRISSMGP GGVNRDNASM DVRSIHPTHY GRICPIETPE
     GHNAGLVNSP TIYARINNYG FLETPFFKVN SGQIQAKAFY LNAQKEQKFK VSPPDLSCSE
     LQFLPLGPTK IESEVPMRKD FKFQRMAATQ IEFIGISPLQ MISVATSLIP FLEHDDANRA
     LMGSNMQRQA VPLLIAERPV VGTGLEGRVI ADSSYIMQTK QSGVISYVSN EQVIVQTFHL
     NN