ID   RPOB1_STIHE             Reviewed;         696 AA.
AC   Q06SE7;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta N-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta N-terminal section;
DE            Short=RNA polymerase subunit beta N-terminal section;
GN   Name=rpoB1; Synonyms=rpoBa;
OS   Stigeoclonium helveticum (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   OCC clade; Chaetophorales; Chaetophoraceae; Stigeoclonium.
OX   NCBI_TaxID=55999;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 441;
RX   PubMed=16944205; DOI=10.1007/s00438-006-0156-2;
RA   Belanger A.-S., Brouard J.-S., Charlebois P., Otis C., Lemieux C.,
RA   Turmel M.;
RT   "Distinctive architecture of the chloroplast genome in the chlorophycean
RT   green alga Stigeoclonium helveticum.";
RL   Mol. Genet. Genomics 276:464-477(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In S.helveticum the gene for this protein is split in
CC       two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; DQ630521; ABF60214.1; -; Genomic_DNA.
DR   RefSeq; YP_764419.1; NC_008372.1.
DR   AlphaFoldDB; Q06SE7; -.
DR   SMR; Q06SE7; -.
DR   PRIDE; Q06SE7; -.
DR   GeneID; 4308442; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.30.150.10; -; 1.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   InterPro; IPR042107; DNA-dir_RNA_pol_bsu_ext_1_sf.
DR   InterPro; IPR019462; DNA-dir_RNA_pol_bsu_external_1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR   Pfam; PF10385; RNA_pol_Rpb2_45; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..696
FT                   /note="DNA-directed RNA polymerase subunit beta N-terminal
FT                   section"
FT                   /id="PRO_0000308261"
SQ   SEQUENCE   696 AA;  79536 MW;  AF337536FB779307 CRC64;
     MAIEKYFIPD FVELQRRSFQ RFLETGLIEE LNKRNPITNS QKDLEILLYP EYYKLAPPRW
     NSLDAILLSK TYSCRLYVPA QLTNRKTKQI MFKWVQLGDI PLMSKRGHFV VNGAARVIIN
     QIVRGPGLYF SEIQYNVDSS KKTLVRRYAA EFISMRGTWL RLSIDKEKMI WAELKKTPKI
     PLVWFLLGMG LTEKILFQSL TQPKRLLYNY LDYLKIDNLA IDVKSQNDAF AKRALLRELL
     LKSKLNSSNI KTLKNSKDFK PLLLVNDIYS EMHSYKEKEN RRYPLNSQNG DLADEKEKIP
     YYANSSLKAL ELIYSKITNT KKITNLNKKF FAKQGQKWIF KKFMNPRTYD LGLHGRLSLN
     KKLSLSLPLS QRTLTMYDVL VATEHLLRVE QGLLGTDDID NLKNRRVRTS SDLIQMQIGI
     GLLRLEKNIR SKFTTIKGVP KINYFFSTKP LNGALKEFFG TNPLSQFMDM INPLAEMTHK
     RRLTTLGPGG VSRDTATMDI RGIHPTHYGR ICPVETPEGK NAGLVNALTT FARVNPEGLI
     ETPFYKVYKG QVQKKAGFFY LSAEKEEKAI IAAGDLNLSK TGFLPKGLIP VRKFDEFTKV
     LRNYVDFMSV SPLQMISVAT SLVPFLEHDD ANRALMGANM QRQAVPLVRS EQAIVGTGIE
     TRIASDSGHV IRAKAGGLVG YVSGQKIRIY TFVSNF