ID   RPOB1_TETOB             Reviewed;         726 AA.
AC   Q1KVX4;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta N-terminal section;
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta N-terminal section;
DE            Short=RNA polymerase subunit beta N-terminal section;
GN   Name=rpoB1; Synonyms=rpoBa;
OS   Tetradesmus obliquus (Green alga) (Acutodesmus obliquus).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus.
OX   NCBI_TaxID=3088;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 393;
RX   PubMed=16638149; DOI=10.1186/1471-2148-6-37;
RA   de Cambiaire J.-C., Otis C., Lemieux C., Turmel M.;
RT   "The complete chloroplast genome sequence of the chlorophycean green alga
RT   Scenedesmus obliquus reveals a compact gene organization and a biased
RT   distribution of genes on the two DNA strands.";
RL   BMC Evol. Biol. 6:37-37(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: In S.obliquus the gene for this protein is split in two.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC       {ECO:0000305}.
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DR   EMBL; DQ396875; ABD48232.1; -; Genomic_DNA.
DR   RefSeq; YP_635950.1; NC_008101.1.
DR   AlphaFoldDB; Q1KVX4; -.
DR   SMR; Q1KVX4; -.
DR   GeneID; 4099817; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.90.1110.10; -; 1.
DR   InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR   InterPro; IPR007642; RNA_pol_Rpb2_2.
DR   InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR   InterPro; IPR007645; RNA_pol_Rpb2_3.
DR   PANTHER; PTHR20856; PTHR20856; 1.
DR   Pfam; PF04561; RNA_pol_Rpb2_2; 2.
DR   Pfam; PF04565; RNA_pol_Rpb2_3; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           1..726
FT                   /note="DNA-directed RNA polymerase subunit beta N-terminal
FT                   section"
FT                   /id="PRO_0000308259"
SQ   SEQUENCE   726 AA;  84049 MW;  6D06CB5010AF1B3E CRC64;
     MYNSNSYIPD LVEIQRQGFF YLLEKGIIEE ISKRNPITCF EKQIEIFFYP QYYRLTKPFY
     SIQQAIFYKK SYVSKFYIPV QLTDRKTKRI FLKWILLAHF PLMTNRGHFL LNGSARVIIN
     QLVRSPGIYF RENMHEIFSN KWSEKPSTTF RRFYADIICL KGTWLRIECD KDFSMWAKMK
     KGPKIPLLWF LLGMGLSEKY ILNSVYKPMN LLQSFTKEME KMQKSKSSKE LKYPYISSTK
     QAWEQIQKLF KLKKTLRKFS FLKKNEEKPK TFIFGQSSTK IEAKVVFSDF AAFNNSVKRE
     NTVFASNDDT FLVQTSFETK KKKNTRKKEN FQKNKTQKTT KLSLQNKEKR VASKLEAYEL
     GRKWFANKFM NPRTYDLGKQ GRWLINKKLG LTIPLEQTTL TALDVLTATD SLMKIEDGFF
     EIDDIDHLKN KRVRTAGEAL QEIFSIGLIR LEKSIRLKLN GNGQQSFFQK TSFFNFDSLM
     STRPINGAFR EFFGTHPLSQ FMDQINPLAE ITHKRRLSSL GPGGVSRDTA TLAIRGIHPS
     HYGRICPIET PEGKNTGLVN SITTYSKISF HGYLESPFYK VYKGQVQRNL GVFYLSPDKD
     DHFQTATPDL NLTPLGFLPK KPIPVRIGKR FVRMKTHKLA LMGVSPLQMI SVATSFIPFL
     EHDDANRALM GSNMQRQAVP LIRPERPLIG TGLEARVVSD SGHAILAKTS GYIVYSSGSK
     IYLYTI